Combinations of PCR and Isothermal Amplification Techniques Are Suitable for Fast and Sensitive Detection of SARS-CoV-2 Viral RNA

Varlamov, Dmitriy A; Blagodatskikh, Konstantin A.; Smirnova, Evgeniya V.; Kramarov, V. M.; Ignatov, Konstantin B.

doi:10.3389/fbioe.2020.604793

Cited by 20 publications

(17 citation statements)

References 25 publications

(42 reference statements)

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“…Recently, a combination of PCR and isothermal nucleic acid amplification known as loop-mediated isothermal amplification (LAMP) techniques was explored. 65 The idea was to introduce the speed of the LAMP techniques and the accuracy of the PCR method in one single platform. It has been shown that combining LAMP and RT-PCR in one assay to develop RT-q(PCR-LAMP) was approximately 100-fold more sensitive than conventional RT-LAMP.…”

Section: Nucleic Acid Amplification Test (Naat)mentioning

confidence: 99%

Diagnostic Approaches For COVID-19: Lessons Learned and the Path Forward

Alafeef

Pan

2022

ACS Nano

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Coronavirus disease 2019 (COVID-19) is a transmitted respiratory disease caused by the infection of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Although humankind has experienced several outbreaks of infectious diseases, the COVID-19 pandemic has the highest rate of infection and has had high levels of social and economic repercussions. The current COVID-19 pandemic has highlighted the limitations of existing virological tests, which have failed to be adopted at a rate to properly slow the rapid spread of SARS-CoV-2. Pandemic preparedness has developed as a focus of many governments around the world in the event of a future outbreak. Despite the largely widespread availability of vaccines, the importance of testing has not diminished to monitor the evolution of the virus and the resulting stages of the pandemic. Therefore, developing diagnostic technology that serves as a line of defense has become imperative. In particular, that test should satisfy three criteria to be widely adopted: simplicity, economic feasibility, and accessibility. At the heart of it all, it must enable early diagnosis in the course of infection to reduce spread. However, diagnostic manufacturers need guidance on the optimal characteristics of a virological test to ensure pandemic preparedness and to aid in the effective treatment of viral infections. Nanomaterials are a decisive element in developing COVID-19 diagnostic kits as well as a key contributor to enhance the performance of existing tests. Our objective is to develop a profile of the criteria that should be available in a platform as the target product. In this work, virus detection tests were evaluated from the perspective of the COVID-19 pandemic, and then we generalized the requirements to develop a target product profile for a platform for virus detection.

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Section: Nucleic Acid Amplification Test (Naat)mentioning

confidence: 99%

Diagnostic Approaches For COVID-19: Lessons Learned and the Path Forward

Alafeef

Pan

2022

ACS Nano

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“…In an LAMP experiment, four to six primers are specially designed to identify six to eight regions of the target sequence. 56 , 57 The amplification begins with an inner primer invasion, then a strand-displacing polymerase elongates the primer and separates the DNA duplex. Subsequently, the first product is displaced by another strand, which is extended by an outer primer.…”

Section: Pathogen-based Laboratory Findings For Covid-19 Detectionmentioning

confidence: 99%

Applications of laboratory findings in the prevention, diagnosis, treatment, and monitoring of COVID-19

Meng

Guo

Zhou

et al. 2021

Sig Transduct Target Ther

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The worldwide pandemic of coronavirus disease 2019 (COVID-19) presents us with a serious public health crisis. To combat the virus and slow its spread, wider testing is essential. There is a need for more sensitive, specific, and convenient detection methods of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Advanced detection can greatly improve the ability and accuracy of the clinical diagnosis of COVID-19, which is conducive to the early suitable treatment and supports precise prophylaxis. In this article, we combine and present the latest laboratory diagnostic technologies and methods for SARS-CoV-2 to identify the technical characteristics, considerations, biosafety requirements, common problems with testing and interpretation of results, and coping strategies of commonly used testing methods. We highlight the gaps in current diagnostic capacity and propose potential solutions to provide cutting-edge technical support to achieve a more precise diagnosis, treatment, and prevention of COVID-19 and to overcome the difficulties with the normalization of epidemic prevention and control.

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“…Specific methods based on quantitative fluorescence polymerase chain reaction (QF-PCR) or isothermal nucleic acid amplification have also been developed (Bustin and Nolan 2020 ; Varlamov et al 2020 ). Nucleic acid amplification tests (NAAT) are highly recommended by the World Health Organization, and they are based on reverse transcription-polymerase chain reaction (RT-PCR).…”

Section: Limits Of Actual Diagnosticsmentioning

confidence: 99%

Nanozymes to fight the COVID-19 and future pandemics

et al. 2021

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In 2019, the pandemic induced by the rapid spread of the severe acute respiratory syndrome coronavirus-2 'SARS-CoV-2', also named coronavirus disease 'COVID-19', has rapidly caused serious health, economic, social, and political issues worldwide. This has been accentuated by asymptomatic carriers, fast viral mutations, incoherent lockdown policies, and limited methods of detection and treatment. Nucleic acid tests for COVID-19 are lengthy and complicated, and the rapid antibody tests produce false-negative diagnoses, particularly in the early stage of infection. Moreover, there are no specific and effective medicines available for treatment. Therefore, rapid diagnostics and therapeutics are urgently required. Here, we argue that nanozymesenzyme-mimicking nanoparticles-could be used for faster, more sensitive, and accurate detection and treatment of the COVID-19. Indeed, nanozymes have high surface areas allowing easier bioconjugation for detection, and nanozymes possess integrated drug-functionalization for treatment. Moreover, the size, composition, and surface chemistry of nanozymes can be rationally designed for improved theranostic applications. COVID-19 historyThe COVID-19 appeared in Wuhan, China, due to emergence of a novel strain of coronavirus (Sallard et al. 2021), which was provisionally named '2019-nCoV' by the World Health Organization (WHO). Later, the virus was renamed 'SARS-CoV-2' by the International Committee

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Combinations of PCR and Isothermal Amplification Techniques Are Suitable for Fast and Sensitive Detection of SARS-CoV-2 Viral RNA

Cited by 20 publications

References 25 publications

Diagnostic Approaches For COVID-19: Lessons Learned and the Path Forward

Diagnostic Approaches For COVID-19: Lessons Learned and the Path Forward

Applications of laboratory findings in the prevention, diagnosis, treatment, and monitoring of COVID-19

Nanozymes to fight the COVID-19 and future pandemics

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