Combinational exposure to hydroxyatrazine increases neurotoxicity of polystyrene nanoparticles on Caenorhabditis elegans

Wang, Yuxing; Yuan, Xiaoan; Zhou, Rong; Bu, Yuanqing; Wang, Dayong

doi:10.1016/j.scitotenv.2023.163283

Cited by 23 publications

(4 citation statements)

References 105 publications

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“…The DAF-7 and DBL-1, two TGF-β ligands, were identified as downstream targets of neuronal GLP-1 in mediating transgenerational PS-S NP toxicity ( Figure 6 E). Neuronal RNAi of daf-7 and dbl-1 caused susceptibility to PS NP toxicity [ 29 , 64 ]. During the regulation of nanoplastic toxicity, neuronal DAF-7 activated the activity of TGF-β receptor DAF-1 in the intestine [ 29 ], and neuronal DBL-1 activated the activity of TGF-β receptor SMA-6 in the intestine [ 64 ].…”

Section: Discussionmentioning

confidence: 99%

“…Neuronal RNAi of daf-7 and dbl-1 caused susceptibility to PS NP toxicity [ 29 , 64 ]. During the regulation of nanoplastic toxicity, neuronal DAF-7 activated the activity of TGF-β receptor DAF-1 in the intestine [ 29 ], and neuronal DBL-1 activated the activity of TGF-β receptor SMA-6 in the intestine [ 64 ]. GLB-10, a globin protein, was further identified as another downstream target of neuronal GLP-1 in mediating transgenerational PS-S NP toxicity ( Figure 6 E).…”

Section: Discussionmentioning

confidence: 99%

“…The Caenorhabditis elegans has been used for the toxicological study of various pollutants [ 25 , 26 , 27 , 28 , 29 ]. At P0-G, toxicity in the development and function of the nervous system could be induced by exposure to polystyrene nanoparticles (PS NPs) [ 30 , 31 ].…”

Section: Introductionmentioning

confidence: 99%

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Sulfonate-Modified Polystyrene Nanoparticle at Precited Environmental Concentrations Induces Transgenerational Toxicity Associated with Increase in Germline Notch Signal of Caenorhabditis elegans

Wang

2023

Toxics

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Recently, the transgenerational toxicity of nanoplastics has received increasing attention. Caenorhabditis elegans is a useful model to assess the transgenerational toxicity of different pollutants. In nematodes, the possibility of early-life exposure to sulfonate-modified polystyrene nanoparticle (PS-S NP) causing transgenerational toxicity and its underlying mechanisms were investigated. After exposure at the L1-larval stage, transgenerational inhibition in both locomotion behavior (body bend and head thrash) and reproductive capacity (number of offspring and fertilized egg number in uterus) was induced by 1–100 μg/L PS-S NP. Meanwhile, after exposure to 1–100 μg/L PS-S NP, the expression of germline lag-2 encoding Notch ligand was increased not only at the parental generation (P0-G) but also in the offspring, and the transgenerational toxicity was inhibited by the germline RNA interference (RNAi) of lag-2. During the transgenerational toxicity formation, the parental LAG-2 activated the corresponding Notch receptor GLP-1 in the offspring, and transgenerational toxicity was also suppressed by glp-1 RNAi. GLP-1 functioned in the germline and the neurons to mediate the PS-S NP toxicity. In PS-S NP-exposed nematodes, germline GLP-1 activated the insulin peptides of INS-39, INS-3, and DAF-28, and neuronal GLP-1 inhibited the DAF-7, DBL-1, and GLB-10. Therefore, the exposure risk in inducing transgenerational toxicity through PS-S NP was suggested, and this transgenerational toxicity was mediated by the activation of germline Notch signal in organisms.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Sulfonate-Modified Polystyrene Nanoparticle at Precited Environmental Concentrations Induces Transgenerational Toxicity Associated with Increase in Germline Notch Signal of Caenorhabditis elegans

Wang

2023

Toxics

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“…Under the normal condition, the L4-larval nematodes were treated with 16–64 mg/L paeoniflorin for 48 h. The lifespan was analyzed as described above. Locomotion behaviors of head thrash and body bend were analyzed to reflect alteration in function of motor neurons ( Wang et al, 2023b ; Hua et al, 2023d ). A head thrash is defined as one swing of nematode body, and a body bend refers to the crawling of one wavelength ( Zhao Y.-Y.…”

Section: Methodsmentioning

confidence: 99%

Paeoniflorin mitigates high glucose-induced lifespan reduction by inhibiting insulin signaling in Caenorhabditis elegans

2023

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In organisms, high glucose can cause several aspects of toxicity, including the lifespan reduction. Paeoniflorin is the major component of Paeoniaceae plants. Nevertheless, the possible effect of paeoniflorin to suppress high glucose toxicity in reducing lifespan and underlying mechanism are largely unclear. Thus, in this study, we examined the possible effect of paeoniflorin in suppressing high glucose (50 mM)-induced lifespan reduction and the underlying mechanism in Caenorhabditis elegans. Administration with 16–64 mg/L paeoniflorin could prolong the lifespan in glucose treated nematodes. Accompanied with this beneficial effect, in glucose treated nematodes, expressions of daf-2 encoding insulin receptor and its downstream kinase genes (age-1, akt-1, and akt-2) were decreased and expression of daf-16 encoding FOXO transcriptional factor was increased by 16–64 mg/L paeoniflorin administration. Meanwhile, the effect of paeoniflorin in extending lifespan in glucose treated nematodes was enhanced by RNAi of daf-2, age-1, akt-1, and akt-2 and inhibited by RNAi of daf-16. In glucose treated nematodes followed by paeoniflorin administration, the increased lifespan caused by daf-2 RNAi could be suppressed by RNAi of daf-16, suggesting that DAF-2 acted upstream of DAF-16 to regulate pharmacological effect of paeoniflorin. Moreover, in glucose treated nematodes followed by paeoniflorin administration, expression of sod-3 encoding mitochondrial Mn-SOD was inhibited by daf-16 RNAi, and the effect of paeoniflorin in extending lifespan in glucose treated nematodes could be suppressed by sod-3 RNAi. Molecular docking analysis indicated the binding potential of paeoniflorin with DAF-2, AGE-1, AKT-1, and AKT-2. Therefore, our results demonstrated the beneficial effect of paeoniflorin administration in inhibiting glucose-induced lifespan reduction by suppressing signaling cascade of DAF-2-AGE-1-AKT-1/2-DAF-16-SOD-3 in insulin signaling pathway.

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Paeoniflorin increases the survival of Pseudomonas aeruginosa infected Caenorhabditis elegans at the immunosuppression stage by activating PMK-1, BAR-1, and EGL-1 signals

Zhang

Wang

2023

Arch. Pharm. Res.

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Combinational exposure to hydroxyatrazine increases neurotoxicity of polystyrene nanoparticles on Caenorhabditis elegans

Cited by 23 publications

References 105 publications

Sulfonate-Modified Polystyrene Nanoparticle at Precited Environmental Concentrations Induces Transgenerational Toxicity Associated with Increase in Germline Notch Signal of Caenorhabditis elegans

Sulfonate-Modified Polystyrene Nanoparticle at Precited Environmental Concentrations Induces Transgenerational Toxicity Associated with Increase in Germline Notch Signal of Caenorhabditis elegans

Paeoniflorin mitigates high glucose-induced lifespan reduction by inhibiting insulin signaling in Caenorhabditis elegans

Paeoniflorin increases the survival of Pseudomonas aeruginosa infected Caenorhabditis elegans at the immunosuppression stage by activating PMK-1, BAR-1, and EGL-1 signals

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