Combination of Ultrasonic-assisted Aqueous Two-phase Extraction with Solidifying Organic Drop-dispersive Liquid–liquid Microextraction for Simultaneous Determination of Nine Mycotoxins in Medicinal and Edible Foods by HPLC with In-series DAD and FLD

Pi, Jiaju; Jin, Peiyi; Zhou, Shiyu; Wang, Liping; Wang, Heping; Huang, Jilong; Gan, Lu; Yuan, Tiefeng; Fan, Huajun

doi:10.1007/s12161-021-02134-w

Cited by 14 publications

(11 citation statements)

References 34 publications

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“…The most harmful and widely distributed mycotoxins include aflatoxin B1 (AFB1), aflatoxin M1 (AFM1), ochratoxin A (OTA), zearalenone (ZEN), deoxynivalenol (DON), and fumonisin B 1 (FB 1 ). Therefore, many analytical methods have been explored to measure these mycotoxins in food samples, such as high-performance liquid chromatography (HPLC) [ 28 , 29 , 30 , 31 ], HPLC-mass spectroscopy (HPLC-MS) [ 32 , 33 , 34 ], enzyme-linked immunosorbent assay (ELISA) [ 35 , 36 , 37 , 38 ], and capillary electrophoresis (CE) [ 39 , 40 , 41 , 42 , 43 ]. Moez’s group employed an aptamer-assisted ultrafiltration purification technique to recognize and separate ochratoxin A (OTA) in green coffee and then use HPLC-fluorescence (FL) detector to realize the quantitative measurement of OTA [ 44 ].…”

Section: Conventional Methods For Determination Of Mycotoxinmentioning

confidence: 99%

Recent Advances in Electrochemiluminescence Biosensors for Mycotoxin Assay

et al. 2023

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Rapid and efficient detection of mycotoxins is of great significance in the field of food safety. In this review, several traditional and commercial detection methods are introduced, such as high-performance liquid chromatography (HPLC), liquid chromatography/mass spectrometry (LC/MS), enzyme-linked immunosorbent assay (ELISA), test strips, etc. Electrochemiluminescence (ECL) biosensors have the advantages of high sensitivity and specificity. The use of ECL biosensors for mycotoxins detection has attracted great attention. According to the recognition mechanisms, ECL biosensors are mainly divided into antibody-based, aptamer-based, and molecular imprinting techniques. In this review, we focus on the recent effects towards the designation of diverse ECL biosensors in mycotoxins assay, mainly including their amplification strategies and working mechanism.

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Section: Conventional Methods For Determination Of Mycotoxinmentioning

confidence: 99%

Recent Advances in Electrochemiluminescence Biosensors for Mycotoxin Assay

et al. 2023

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“…Another recent advancement in multi-toxin analysis utilised two-phase extraction coupled with solidifying organic dropdispersible liquid-liquid microextraction (Pi et al, 2022). The method was validated for the following mycotoxins: aflatoxins (AFB 1 , AFB 2 , AFG 1 , AFG 2 , and AFM 1 ), patulin (PAT), ZEN, OTA, and DON at the same time using HPLC with DAD and FLD in series.…”

Section: Chromatography With Non-ms Detectionmentioning

confidence: 99%

“…Hence, findings from the research indicated that the proposed multitoxin IAC technique can be a reliable and cost-effective methodology for future mycotoxin analysis, screening, and standardisation in maize-based foods. Like Pi et al's (2022) multi-toxins analysis, the method is applicable in conducting large-scale analysis while determining multiple mycotoxins in a large number of samples. Palma et al (2022) developed and validated a modified methodology for extracting, purifying, and quantifying total aflatoxins, including AFB 1 , AFB 2 , AFG 1 , and AFG 2 in smoked chili pepper (merkén).…”

Section: Chromatography With Non-ms Detectionmentioning

confidence: 99%

Developments in mycotoxin analysis: an update for 2021-22

Tittlemier¹,

Cramer

DeRosa

et al. 2023

WMJ

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This review summarises developments published in the period from mid-2021 to mid-2022 on the analysis of a variety of matrices for mycotoxins. Important developments in all aspects of mycotoxin analysis, from sampling and quality assurance/quality control of analytical results, to the various detection and quantitation technologies ranging from single mycotoxin biosensors to comprehensive instrumental methods are presented and discussed. This non-exhaustive summary and associated discussion covers such technology as chromatography with targeted or non-targeted high resolution mass spectrometry, detection other than mass spectrometry such as fluorescence or diode array detection, biosensors, as well as assays using alternatives to antibodies. This collaborative critical review intends to guide readers to relevant research by briefly presenting the most important developments in mycotoxin determination published in the past year. This review also relays limitations of the presented methodologies, in order to provide a fulsome assessment of the analytical developments.

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“…Nowadays, there has been an increasing public and professional attention on medicinal foods including some Chinese medicinal materials (CMMs) in terms of their pivotal contribution to human nutrition and health; meanwhile, aflatoxins (AFs) contaminations in CMMs also began to raise more concerns and cautions (Pi et al ., 2022; Ying et al ., 2018). Aflatoxin B1 (AFB1), B2 (AFB2), G1 (AFG1) and G2 (AFG2) are the most common types of AFs, which are mainly produced by two aflatoxigenic strains of Aspergillus flavus and A. parasiticus (Amirkhizi et al ., 2017; Wang et al ., 2019, 2022; Agriopoulou et al ., 2020).…”

Section: Introductionmentioning

confidence: 99%

“…These toxic AFs especially AFB1 have potential health risk to human, which can induce serious acute or chronic diseases even liver cancer and strict limitation standards in food have been officially set in USA, Europe, China and other countries (Bernáldez et al ., 2017; Annunziata et al ., 2021). Considering the possible occurrence of AFs contamination in some medicinal foods such as lotus seed and apricot kernel (Liao et al ., 2020; Pi et al ., 2022), the maximum permissible standard of for AFB1 and the total aflatoxins (AFTOT) of AFB1 + AFB2 + AFG1 + AFG2 have been stipulated in Chinese Pharmacopoeia Commission.…”

Section: Introductionmentioning

confidence: 99%

Influence of Aspergillus parasiticus on aflatoxins production and quality change in Chrysanthemum morifolium at various storage conditions

Chen,

Wang,

Huo

et al. 2024

Int J of Food Sci Tech

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SummaryIn this study, a full factorial design with three temperature levels (20, 30 and 40 °C), three moisture content (MC) levels (8%, 15% and 22%), and four storage times (7, 14, 21 and 28 days) was used to investigate the changes of aflatoxins (AFs), active constituents, and antioxidant activities in Hangbaiju (HBJ, Chrysanthemum morifolium) after simulated inoculation with Aspergillus parasiticus. The results showed that AFs could be produced in the range of 20~30 °C and 8~22% MC after mould infection, but both mould growth and AFs production were inhibited under the tested MC condition at 40 °C. The analysis of variance (anova) indicated that the temperature, time, interaction between the temperature and time, and interaction between MC and time had significant effects on the total aflatoxins (AFTOT) accumulation. Under 20 and 30 °C, AFTOT in inoculated HBJ usually increased with the increase of MC and storage time, while its active constituents and antioxidant activities decreased with the increase of AFTOT under corresponding storage conditions. Pearson correlation analysis suggested that relatively stronger negative correlations were observed between luteolin‐7‐O‐glucoside (LUT7G), total phenolic content (TPC), total flavonoid content (TFC), and AFTOT. Grey correlation analysis (GCA) showed that antioxidant activity was highly linked to the content of active constituents, and the characteristic flavonoid LUT7G exhibited the highest grey correlation degrees (GCDs) in the three antioxidant activities. The AFTOT reached the highest level (31.15 μg kg−1) after 28 days of storage at 30 °C and 22% MC; meanwhile, LUT7G, TPC and TFC decreased by 93.66%, 20.77% and 55.50%, respectively, and all the three antioxidant activities indicated more than 25% reduction. This is the first study about the changes and relationships of AFTOT, active constituents and bioactivities in the medicinal food influenced by A. parasiticus under different storage conditions. These results provide important references to prevent AFs production and quality control for HBJ and other medicinal foods influenced by A. parasiticus in storage strategy.

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Combination of Ultrasonic-assisted Aqueous Two-phase Extraction with Solidifying Organic Drop-dispersive Liquid–liquid Microextraction for Simultaneous Determination of Nine Mycotoxins in Medicinal and Edible Foods by HPLC with In-series DAD and FLD

Cited by 14 publications

References 34 publications

Recent Advances in Electrochemiluminescence Biosensors for Mycotoxin Assay

Recent Advances in Electrochemiluminescence Biosensors for Mycotoxin Assay

Developments in mycotoxin analysis: an update for 2021-22

Influence of Aspergillus parasiticus on aflatoxins production and quality change in Chrysanthemum morifolium at various storage conditions

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