Combination of UC-3500 and UF-5000 as a quick and effective method to exclude bacterial urinary tract infection

Chen, Yabin; Zhang, Zhishan; Diao, Yanyan; Wang, Wanni; Zhu, Yan; Li, Jiaming; Wang, Gangqiang; Zhao, Yuan; Wang, Qisheng; Wu, Yibo; Jing, Jing

doi:10.1016/j.jiac.2023.03.008

Cited by 4 publications

(3 citation statements)

References 11 publications

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“…The urine formed element analyzer is widely used in clinical practice. The screening performance of urine testing for UTIs is widely recognized ( 18 - 21 ). In addition, automated urine sediment analyzers have been used to differentiate between glomerular hematuria (GH) and non-glomerular hematuria (NGH) ( 22 ).…”

Section: Discussionmentioning

confidence: 99%

Performance analysis of urine formed element analyzer EH-2090 was found to have good accuracy in detecting RBCs and WBCs when compared to manual microscopic

Bai,

Xu,

2024

Transl Androl Urol

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Background EH-2090 is Mindray’s new-generation fully automatic urine formed element analyzer (hereinafter referred to as EH-2090). Currently, there are no studies on EH-2090, so we evaluated the analytical and clinical performance of this instrument to verify that it can meet daily clinical needs, and used manual microscopy as a reference method. Methods The analytical performance of the EH-2090 was first evaluated for repeatability, linearity, reproducibility, and carryover. We collected urine samples from outpatient and inpatient departments of Peking University Shenzhen Hospital. Uncentrifuged urine was compared with the EH-2090 using the Fuchs–Rosenthal counting method—a quantitative reference method for microscopy—for comparative studies in terms of red blood cell (RBC) and white blood cell (WBC) counting accuracy. Passing-Bablok regression analysis was performed for RBC and WBC counts. Two laboratory technicians performed centrifugation and manual analysis (microscopy) to evaluate its performance at detecting RBCs, WBCs, and casts, sensitivities and specificities were calculated. Results The EH-2090’s between-run reproducibility, within-day reproducibility, between-day reproducibility, and within-laboratory reproducibility for formed components of urine all met the laboratory requirements. There was a good correlation between the counting accuracy of RBCs (r=0.965, P<0.0001) and WBCs (r=0.894, P<0.0001) by the EH-2090 and the Fuchs-Rosenthal method. The positive coincidence rates of RBC and manual microscopy were 86.08% and 92.41%, respectively, and the negative coincidence rates were 88.39% and 85.81%, respectively. The positive coincidence rates before and after the WBC review were 89.33% and 92.00%, respectively, whereas the negative ones were 77.64% and 83.23%, respectively. The positive coincidence rates before and after cast review were 77.78% and 82.05%, respectively, and the negative ones were 97.09% and 93.60%, respectively. Conclusions The EH-2090 has good analytical and clinical performance. Its RBC and WBC counting accuracy correlates well with the quantitative reference method of microscopy.

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Section: Discussionmentioning

confidence: 99%

Performance analysis of urine formed element analyzer EH-2090 was found to have good accuracy in detecting RBCs and WBCs when compared to manual microscopic

Bai,

Xu,

2024

Transl Androl Urol

View full text Add to dashboard Cite

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“…WBCs in urine can be used as indicators of UTIs ( 18 , 19 ). There are no or very few leukocytes in the urine of normal people; when elevated in urine, leukocytes are mostly neutrophils.…”

Section: Discussionmentioning

confidence: 99%

Performance evaluation and clinical application of the UA-5600 automatic dry chemistry urine analyzer in renal diseases

Liu,

Zheng,

Wang

et al. 2024

Transl Androl Urol

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“…Nevertheless, the test strip of UC-3500 can simultaneously measure leukocyte esterase and nitrite. Furthermore, it can be used in conjunction with an automatic urine particle analyzer to determine the presence of UTI [28]. This additional information in the test results of these patients can help us assess whether the UACR measured by UC-3500 might be affected by the concomitant presence of UTI.…”

Section: Discussionmentioning

confidence: 99%

Albuminuria Is Affected by Urinary Tract Infection: A Comparison between Biochemical Quantitative Method and Automatic Urine Chemistry Analyzer UC-3500

Chou,

Yang,

Chang

et al. 2023

Diagnostics

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The automated urine reagent strip test is a cost-effective tool for detecting albuminuria in patients. However, prior research has not investigated how urinary tract infections (UTIs) affect the test’s accuracy. Therefore, this study aims to assess the impact of UTIs on albuminuria diagnosis using both the biochemical quantitative method and the test strip method of the Fully Automatic Urine Chemistry Analyzer, UC-3500 (Sysmex, Kobe, Japan). From March to December 2019, we prospectively collected midstream urine from adult female UTI patients before and after one week of cephalexin treatment. The urine samples were subjected to culture, routine urinalysis, and albuminuria diagnosis using the biochemical quantitative method and UC-3500. Albuminuria was defined as a urine albumin to creatinine ratio (UACR) ≥ 30 mg/g in the biochemical quantitative method. The results were compared between the two methods. Among fifty-four female patients (average age: 50.5 ± 4.4 years) with UTIs, 24 (44.44%) had transient albuminuria. The quantitative UACR significantly decreased after one week of antibiotic treatment (median: 53 mg/g to 9 mg/g; median difference: −0.54, p < 0.0001). UC-3500 exhibited a higher false positive rate for diagnosing albuminuria during UTIs (42%) compared to after treatment (19%). Its agreement with the biochemical quantitative method was moderate during UTI (κ = 0.49, 95% confidence interval [CI]: 0.24–0.73) and good after treatment (κ = 0.65, 95% CI: 0.45–0.86). UC-3500’s accuracy in diagnosing albuminuria is influenced by UTIs, leading to either transient albuminuria or a false positive reaction of the test strip. UTI should be excluded or treated before its application in albuminuria screening.

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Combination of UC-3500 and UF-5000 as a quick and effective method to exclude bacterial urinary tract infection

Cited by 4 publications

References 11 publications

Performance analysis of urine formed element analyzer EH-2090 was found to have good accuracy in detecting RBCs and WBCs when compared to manual microscopic

Performance analysis of urine formed element analyzer EH-2090 was found to have good accuracy in detecting RBCs and WBCs when compared to manual microscopic

Performance evaluation and clinical application of the UA-5600 automatic dry chemistry urine analyzer in renal diseases

Albuminuria Is Affected by Urinary Tract Infection: A Comparison between Biochemical Quantitative Method and Automatic Urine Chemistry Analyzer UC-3500

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