Combination of rice blast resistance genes in the genotypes of russian rice varieties with the use of marker assisted selection

Kostylev, P. I.; Иванович, Костылев Павел; Краснова, Е. В.; Викторовна, Краснова Елена; Редькин, А. А.; Александрович, Редькин Александр; Dubina, Elena; Викторовна, Дубина Елена; Mukhina, Zhanna; Михайловна, Мухина Жанна

doi:10.17816/ecogen15354-63

Cited by 4 publications

(4 citation statements)

References 20 publications

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“…Small caryopses develop on the affected plants, the setting of seeds in the panicle decreases, the grain yield lowers to 40%, and the quality of products deteriorates sharply. In the presence of favorable conditions, the blast pathogen may reduce the rice yield by up to 45%, and in the years of epiphytotic yield damage can reach 100% [6][7][8].…”

Section: Introductionmentioning

confidence: 99%

“…This difficulty is determined by the race-forming processes continuously going on in the pathogen. With a change in the cultivated assortment of rice, a strict selection takes place for the survival of the P. oryzae pathogen itself, as a result of which new races, pathotypes of the blast pathogen appear [6,8]. To conduct successful breeding for resistance to this disease, it is necessary to have a clear understanding of the evolution of the pathogen; knowing its intrapopulation structure, virulence gene pool and interaction features in the host-parasite system; constantly conducting a wide search for new sources of resistance and creating a bank of resistance genes and pathogen strains.…”

Section: Introductionmentioning

confidence: 99%

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Molecular and morphological traits of Pyricularia oryzae Cav. in the south of Russia

et al. 2022

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Based on molecular genetic approaches, a study of the biodiversity of the phytopathogenic fungus Pyricularia oryzae Cav. was carried out in the south of Russia, the classification of morphological and cultural traits of the pathogen was carried out. In 2020, monitoring was carried out and 9 strains of the pathogen were isolated from the affected herbarium material collected from the fields of five ecological rice-growing zones of Krasnodar region (Krasnoarmeisky, Kalininsky, Abinsky, Slavyansky districts, Krasnodar). Using a multiplex PCR system based on fragment analysis, among the studied strains of Pyricularia oryzae Cav. 5 genotypes were identified in 2020, each of which is was characterized by a unique genetic profile. Their «DNA-passports» have been compiled. The studied isolates of the blast pathogen were characterized by morphological and cultural traits. 6 morphotypes of the pathogen were identified. Classification of the pathogen by morphological, cultural and genetic traits will allow obtaining scientifically grounded and comparable data that can be used in the analysis of the interaction of blast strains with rice plants on different varieties and in different agro-technological conditions in order to improve and rationalize agricultural activities. In addition, the studied approaches open up the possibility of using data in breeding, allowing the identification of pathogen forms that infect certain varieties.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Molecular and morphological traits of Pyricularia oryzae Cav. in the south of Russia

et al. 2022

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“…One of the strategies for developing stable blast resistance is combining several resistance genes in the genotype. A number of successful breeding programs have been carried out to develop blast-resistant rice varieties by gene pyramiding using marker breeding [5][6][7][8]. An important resource for accelerated genetic stabilization of the obtained breeding material is experimental haploidy [9][10][11][12][13].…”

Section: Introductionmentioning

confidence: 99%

Development of rice pre-breeding resources with blast resistance

et al. 2020

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Within the framework of this study, the first Russian-Chinese joint program for development of rice varieties with long-term blast resistance was launched on the basis of rice germplasm exchange and the subsequent comprehensive study of the obtained breeding samples, hybridization of Russian and Chinese varieties with specified traits. The genetic diversity of the Chinese rice samples obtained by exchange was studied by biological and morphological traits of plants, taxonomic belonging to a botanical variety, elements of the yield structure, and resistance to lodging. 31 hybrid combinations (F1) from the crossing of Russian and Chinese varieties were used to obtain the BC1 generation. The technologies of cultivation of isolated rice anthers in vitro have been optimized in relation to Chinese genotypes in order to accelerate the genetic stabilization of breeding material obtained from crossing. Phenotyping of Russian and Chinese breeding samples was carried out on the basis of resistance to the local population of the blast pathogen (Magnaporthe grisea (T.T. Hebert) M.E. Barr)), on natural and artificial infectious backgrounds. DNA identification of genes for blast resistance was carried out for the same breeding samples. Based on the experimental data obtained, promising samples were selected - prototypes of new rice varieties.

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“…Seeding is very important for high yields and feed efficiency. Recent studies show that very high seeding are important to yield (Sevik and Cetin, 2015;Cetin et al, 2018;Tiwari et al, 2014;Torres et al, 2010;Goncharova et al, 2018;Kostylev et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

Introduction of the Sub1 gene into the Russian rice varieties using the polymerase chain reaction (PCR) methods

Dubina¹,

Алабушев²,

Kostylev³

et al. 2018

Afr. J. Agric. Res.

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Selection done using molecular markers has spread to all types of agricultural plants and animals. Marker assisted selection (MAS) is safe for ecology and food products consumed. The purpose of the study is to develop Sub1A gene (which determines tolerance to prolonged flooding) in the Russian rice varieties using DNA-markers and Polymerase chain reaction (PCR) analysis. A large volume of prebreeding rice material (F2-F4, BC2F2) was created with tolerance genes for prolonged flooding, to control weed in rice agrophytocenosis. The forms with 120-130-day vegetation period were selected. An analysis of the co-inheritance of simple sequence repeat (SSR) markers Sub1Aq, Sub1C173, Sub1A203 with the Sub1 gene was carried out. It revealed the codominance of the selected microsatellites. They can be used to identify and visualize the allelic state of a given target gene in the breeding material. The recurrent parental rice forms, donors of the gene Sub 1 and the hybrids of F2 and ВС2F1 population were tested on tolerance to prolonged flooding. The gene is effective in ensuring resistance to this stress factor within 2 to 3 weeks and it can be recommended for the breeding programs to develop modern genetic resources of rice tolerant to prolonged and deep flooding for weed control on rice fields.

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Combination of rice blast resistance genes in the genotypes of russian rice varieties with the use of marker assisted selection

Cited by 4 publications

References 20 publications

Molecular and morphological traits of Pyricularia oryzae Cav. in the south of Russia

Molecular and morphological traits of Pyricularia oryzae Cav. in the south of Russia

Development of rice pre-breeding resources with blast resistance

Introduction of the Sub1 gene into the Russian rice varieties using the polymerase chain reaction (PCR) methods

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