Combination of quantum dot fluorescence with enzyme chemiluminescence for multiplexed detection of lung cancer biomarkers

Li, Huan; Cao, Zhijuan; Zhang, Yuhao; Lau, Choiwan; Lu, Jianzhong

doi:10.1039/c0ay00284d

Cited by 32 publications

(19 citation statements)

References 38 publications

(22 reference statements)

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“…A comparison with ELISA showed that the application of quantum dots as a marker makes it possible to decrease IC 50 by a factor of 4 (0.4 and 0.1 ng/mL of zearalenone, respectively) [68]. The luminescence signal of quantum dots was used simultaneously with the chemiluminescence signal of an enzyme marker for the simultaneous determination of analytes in blood serum [69]. The introduction of quantum dots into the composition of microspheres increased their luminescence intensity and simplified bioconjugation [70].…”

Section: Immunochemical Methods Usedmentioning

confidence: 99%

Contemporary trends in the development of immunochemical methods for medical analysis

Goryacheva

2015

J Anal Chem

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The review is dedicated to contemporary trends in the development of immunochemical meth ods, the position of these methods in clinical analysis, and the applications of these methods. The tendencies toward miniaturization and the simultaneous determination of several analytes are considered. The prospects of applying nanosized markers, the requirements imposed on them, and limitations are discussed in detail. Predictions of the subsequent development of immunochemical methods in the area of clinical analysis are made.

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Section: Immunochemical Methods Usedmentioning

confidence: 99%

Contemporary trends in the development of immunochemical methods for medical analysis

Goryacheva

2015

J Anal Chem

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“…Accordingly, investigating the amounts of CEA plays an important role towards clinical purpose. Currently, immunoassays and related techniques have been considered as major analytical methods towards detections of CEA mainly including radioimmunoassay, enzyme immunoassay, chemiluminescence immunoassay and fluorescence immunoassay (Lang et al, 2014;Li et al, 2010;Yu et al, 2013;Yuan et al, 2001).…”

Section: Introductionmentioning

confidence: 99%

Selectively assaying CEA based on a creative strategy of gold nanoparticles enhancing silver nanoclusters' fluorescence

Yang

Zhuo

Zhu

et al. 2015

Biosensors and Bioelectronics

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“…Taking into account the sensitivities of the sensors based on P/graphite and P/graphene as well as their limits of determination for NSE and CEA, and the working concentration ranges, the best to use for simultaneous pattern recognition of NSE and CEA will be the sensor based on P/graphene. Compare with the results proposed previously for simultaneous assay of NSE and CEA using quantum dots based fluorescence and chemiluminescence immunoassay (Li et al ., ; Cao et al ., ; Li et al ., ), there were recorded lower limits of determination (in the range of pg/ml compared with ng/ml reported previously (Li et al ., ; Cao et al ., ; Li et al ., )), and higher sensitivities. The stochastic method allows direct assay of the analytes in whole blood samples, while the previously reported methods (Li et al ., ; Cao et al ., ; Li et al ., ) required processed samples, namely serum samples for the simultaneous assay of the analytes.…”

Section: Resultsmentioning

confidence: 99%

Pattern recognition of neuron specific enolase and carcinoembryonic antigen in whole blood samples

Staden

Comnea-Stancu

Surdu-Bob

et al. 2015

J of Molecular Recognition

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New tools and methods for pattern recognition of neuron specific enolase (NSE) and carcinoembryonic antigen (CEA) were proposed for the screening of whole blood samples. The new tools were based on stochastic sensors designed using nanoporous gold microspheres, graphite, graphene, diamond paste as well as α-CDs, and 5,10,15,20-tetraphenyl-21H,23H-porphyrin. The best sensor for the assay of CEA was the one based on P/graphite (the limit of determination was 16 fg/ml and sensitivity was 2.32 × 10(7) s mg(-1) ml), while for the assay of NSE the, best sensor was the one based on P/graphene (the limit of determination was 7.45 pg/ml and sensitivity was 2.49 × 10(8) s mg(-1) ml). The sensor of choice for simultaneous detection of NSE and CEA is the one based on P/graphene because we need high sensitivity and low limit of determination for NSE. To our knowledge, this is the only one screening test for early detection of lung cancer, by identification of NSE and CEA in whole blood samples.

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Combination of quantum dot fluorescence with enzyme chemiluminescence for multiplexed detection of lung cancer biomarkers

Cited by 32 publications

References 38 publications

Contemporary trends in the development of immunochemical methods for medical analysis

Contemporary trends in the development of immunochemical methods for medical analysis

Selectively assaying CEA based on a creative strategy of gold nanoparticles enhancing silver nanoclusters' fluorescence

Pattern recognition of neuron specific enolase and carcinoembryonic antigen in whole blood samples

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