Combination of Plant Growth Regulators, Maltose, and Partial Desiccation Treatment Enhance Somatic Embryogenesis in Selected Malaysian Rice Cultivar

Ming, NG Ja; Mostafiz, Suraiya Binte; Johon, Nur Syafiqoh; Zulkifli, Nur Saliha Abdullah; Wagiran, Alina

doi:10.3390/plants8060144

Cited by 23 publications

(18 citation statements)

References 16 publications

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“…), drying at 25 ± 1 °C for 2 h was shown to significantly improve the SE induction rate and maturation rate (Natarajan et al 2020). Similarly, a partial drying treatment was shown to significantly improve the regeneration ability of a Malaysian rice cultivar (Oryza sativa; Ming et al 2019). In our study, the immature embryos placed onto MS½ medium supplemented with 1 mg L −1 ABA after drying showed the best maturation rate, and the cotyledons were elongated and milky white.…”

Section: Maturation Of Somatic Embryosupporting

confidence: 58%

“…In plant cell division and differentiation, auxin and cytokinin are key regulators, and the balance between them plays an important role in SE development (Binte and Wagiran 2018; Ming et al 2019). Although the existence of 2,4-D induced the embryogenic potential of callus, cytokinin was required for the development of SEs during subculturing (Wang et al 2014).…”

Section: Differentiation Of Embryogenic Callusmentioning

confidence: 99%

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Indirect somatic embryogenesis and regeneration of Fraxinus mandshurica plants via callus tissue

et al. 2020

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supplemented with 0.15 mg•L −1 naphthalene acetic acid. The highest callus proliferation coefficient (240.5) was obtained on McCown's Woody Plant Medium containing 0.1 mg•L −1 6-benzyl adenine and 0.15 mg•L −1 2, 4-dichlorophenoxyacetic acid. The highest number of SEs (1020.5 g −1 fresh weight) was obtained on MS½ medium supplemented with 1 mg•L −1 6-benzyladenine. The highest number of cotyledon embryos (397/g fresh weight) was obtained by incubating materials on medium containing 1 mg•L −1 abscisic acid and then applying a drying treatment. The cotyledon embryos were milky white, uniformly sized (average length 4.7 mm), and 80% of them were normal. The SE rooting percentage on ½MS medium containing 0.01 mg•L −1 NAA was 37.5%. Overall, the germination percentage of SEs was 26.4%, and complete regenerated plants were obtained after transplanting and acclimation. These results provide more possibilities for the preservation and breeding of F. mandshurica.

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Section: Maturation Of Somatic Embryosupporting

confidence: 58%

Section: Differentiation Of Embryogenic Callusmentioning

confidence: 99%

Indirect somatic embryogenesis and regeneration of Fraxinus mandshurica plants via callus tissue

et al. 2020

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“…Moreover, variation in callus induction on different concentrations of growth regulators depends on the morphogenetic response which varies according to spatial and temporal cell distributions, and on physiological and developmental stages. Even there were the nodules in compact callus of Tagetes erecta from 2,4 D effect in medium Benítez-García et al (2014) and Ming et al (2019).…”

Section: Texture Of Callusmentioning

confidence: 95%

Effect of Subculture Frequency Toward Growth And Carotenoid Content from Tomato (Lycopersicon Esculentum Mill.) Callus

et al. 2020

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Tomatoes (Lycopersicon esculentum Mill.) are a source of carotenoids they are easy to find. These compounds function as precursors of vitamin A, antioxidant, and prevent cancer. The extraction of carotenoid compounds for commercial products usually uses fresh plants, which are less efficient and require a lot of raw materials. The supply of these raw materials can be done through tissue culture. The frequency of subculture or supply of nutrients in tissue culture is very influential on the content of callus carotenoids produced. This study aimed to determine the effect of subculture frequency on growth, development and callus carotenoid content and to find out the right frequency of subculture to produce callus with optimal growth, development and carotenoid content. The design this study was a single Completely Randomized Design (CRD) with 4 treatments of subculture frequency and 5 replications. The data obtained were analyzed by ANOVA at the 95% test level followed by DMRT in case a significant different was found The results showed subculture frequency affected growth, development and carotenoid content of callus Lycopersicon esculentum. Mill. The most optimal treatment to induce growth and production of carotenoids in this study was treatment of thrice subculture while the most optimal treatment in inducing development was the twice subculture treatment.

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“…One possible theory is that the NO-3 :NH4 + ratio is altering cell sensitivity to auxin hence regulating the uptake or metabolism of hormones (Davies, 1987;Grimes and Hodges, 1990). Callus formation from rice has been shown to be highly affected by media and genotypes (Ng et al, 2019). N6 medium is more suitable for japonica rice, as it has high content of KNO3 and (NH4)SO4 compared to MS. On the other hand, indica rice requires a lower level of NH 4+ (Kaushal et al, 2014).…”

Section: Effect Of 24-dicholorophenoxyacetic Acid (24-d) and Media On Callus Formationmentioning

confidence: 99%

CALLUS FORMATION AND PLANT REGENERATION FROM IMMATURE EMBRYOS OF PIGMENTED UPLAND RICE (Oryza sativa cv. Tadong)

et al. 2021

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The application of biotechnology in upland rice improvement programs depends on the availability of efficient regeneration protocols. Although protocols for shoot regeneration of upland rice are available, none has been reported for pigmented cultivars. This study reports on a protocol for callus induction and regeneration of Tadong, a pigmented upland rice cultivar from Sabah. For callus induction, immature embryos were cultured on media containing 2,4-Dichlorophenoxyacetic (2,4-D) at various concentrations (0 – 2.5 mg/L) and on different types of media (MS; MSB5; N6B5; N6). To induce shoot regeneration, callus explants were cultured on MS medium supplemented with combinations of 6-Benzylaminopurine (BAP) at various concentrations (0 – 3.0 mg/L) and 1-Naphthaleneacetic acid (NAA) at 1.0 mg/L. To induce shoot development, callus explants were pre-treated with Thidiazuron (TDZ) at various concentrations (0-1.0 mg/l) and exposed to different desiccation periods (0 – 72 hours). 2,4-Dichlorophenoxyacetic at 2.5 mg/L and N6B5 medium resulted in the highest percentages of explant forming callus which were 60.3 ± 17.0 % and 58.7 ± 9.8 % respectively. The regeneration media failed to induce shoot on callus explants, instead, green spots were formed on the surface of the callus. The green spots were stimulated to develop into shoots when the callus explants were pre-treated with 0.5 mg/L TDZ or exposed to partial desiccation for 24 h, the percentages of explant forming shoot were 35.7 ± 4.8 % and 47.7 ± 6.8 % respectively. Shoots developed into complete plants on hormone-free MS medium and acclimatized.

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Combination of Plant Growth Regulators, Maltose, and Partial Desiccation Treatment Enhance Somatic Embryogenesis in Selected Malaysian Rice Cultivar

Cited by 23 publications

References 16 publications

Indirect somatic embryogenesis and regeneration of Fraxinus mandshurica plants via callus tissue

Indirect somatic embryogenesis and regeneration of Fraxinus mandshurica plants via callus tissue

Effect of Subculture Frequency Toward Growth And Carotenoid Content from Tomato (Lycopersicon Esculentum Mill.) Callus

CALLUS FORMATION AND PLANT REGENERATION FROM IMMATURE EMBRYOS OF PIGMENTED UPLAND RICE (Oryza sativa cv. Tadong)

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