Abstract:This study assessed the occurrence of an enhancing inhibitory effect of the combined application of Origanum vulgare L. essential oil and lactic acid against Staphylococcus aureus by the determination of Fractional Inhibitory Concentration (FIC) index and cell viability in meat broth and meat model. Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) of the oil was 0.6 and 1.25 µL.mL-1, respectively. Lactic acid showed MIC and MBC of 2.5 and 5µL.mL-1, respectively. FIC indices o… Show more
“…The synergistic antifungal effects of thyme essential oil and LCFS are explained though the interactions between essential oil and its phenolic compounds with lactic acids as proposed earlier by Barros et al (2012). Lactic acid in LCFS provides the acidic environment that increases the hydrophobicity of essential oils.…”
Section: Synergistic Antifungal Effects Of Thyme Essential Oil and mentioning
confidence: 82%
“…The synergistic antifungal effects between essential oil and LCFS were evaluated by using FIC index by following the method of Barros, Conceição, Gomes Neto, Costa, and Souza (), with slight modifications. The tested combinations (thyme essential oil + LCFS) were; ½ MIC + ½ MIC, ½ MIC + ¼ MIC, ½ MIC + ⅛ MIC, ¼ MIC + ½ MIC, ¼ MIC + ¼ MIC, ¼ MIC + ⅛ MIC, ⅛ MIC + ½ MIC, ⅛ MIC + ¼ MIC, and ⅛ MIC + ⅛ MIC.…”
Section: Methodsmentioning
confidence: 99%
“…The synergistic antifungal effects between essential oil and LCFS were evaluated by using FIC index by following the method of Barros, Conceição, Gomes Neto, Costa, and Souza (2012), with slight modifications. The tested combinations (thyme essential…”
Section: In Vitro Synergism Between Thyme Essential Oil and Lcfsmentioning
confidence: 99%
“…Furthermore, the phenolic compounds present in the essential oil are known to cause sub-lethal injury to the cell membranes through the disruption of proton motive force (H 1 ) and the interference of cellular energy production. This injury makes the cells more vulnerable to the acidic environment caused by lactic acids and organic acids present in LCFS (Barros et al, 2012). The degree of degradation increased with increase in the concentration of the mixture applied, suggesting that the effect was derived from the addition of the mixture.…”
Section: Synergistic Antifungal Effects Of Thyme Essential Oil and mentioning
This study evaluates the synergistic antifungal effects between thyme essential oils and Lactobacillus plantarum cell-free supernatant (LCFS) against Penicillium spp. and in situ antifungal activity in rice grains. Thyme essential oil and LCFS showed remarkable antifungal activities against Penicil-
Practical applicationsEssential oils are used as preservative in food industry and high concentration of essential oil is associated with negative organoleptic characteristics. This study presented a novel approach for synergistic antifungal effects by using the combination of thyme essential oil and Lactobacillus plantarum cell-free supernatant (LCFS) against Penicillium spp. and systematic evaluation of the antifungal effect by using fractional inhibitory concentration (FIC) index method. This approach will be a role model for future research on synergism and overcome the major drawbacks of using live bacteria and the negative effects arising from antimicrobial activities of essential oils.
“…The synergistic antifungal effects of thyme essential oil and LCFS are explained though the interactions between essential oil and its phenolic compounds with lactic acids as proposed earlier by Barros et al (2012). Lactic acid in LCFS provides the acidic environment that increases the hydrophobicity of essential oils.…”
Section: Synergistic Antifungal Effects Of Thyme Essential Oil and mentioning
confidence: 82%
“…The synergistic antifungal effects between essential oil and LCFS were evaluated by using FIC index by following the method of Barros, Conceição, Gomes Neto, Costa, and Souza (), with slight modifications. The tested combinations (thyme essential oil + LCFS) were; ½ MIC + ½ MIC, ½ MIC + ¼ MIC, ½ MIC + ⅛ MIC, ¼ MIC + ½ MIC, ¼ MIC + ¼ MIC, ¼ MIC + ⅛ MIC, ⅛ MIC + ½ MIC, ⅛ MIC + ¼ MIC, and ⅛ MIC + ⅛ MIC.…”
Section: Methodsmentioning
confidence: 99%
“…The synergistic antifungal effects between essential oil and LCFS were evaluated by using FIC index by following the method of Barros, Conceição, Gomes Neto, Costa, and Souza (2012), with slight modifications. The tested combinations (thyme essential…”
Section: In Vitro Synergism Between Thyme Essential Oil and Lcfsmentioning
confidence: 99%
“…Furthermore, the phenolic compounds present in the essential oil are known to cause sub-lethal injury to the cell membranes through the disruption of proton motive force (H 1 ) and the interference of cellular energy production. This injury makes the cells more vulnerable to the acidic environment caused by lactic acids and organic acids present in LCFS (Barros et al, 2012). The degree of degradation increased with increase in the concentration of the mixture applied, suggesting that the effect was derived from the addition of the mixture.…”
Section: Synergistic Antifungal Effects Of Thyme Essential Oil and mentioning
This study evaluates the synergistic antifungal effects between thyme essential oils and Lactobacillus plantarum cell-free supernatant (LCFS) against Penicillium spp. and in situ antifungal activity in rice grains. Thyme essential oil and LCFS showed remarkable antifungal activities against Penicil-
Practical applicationsEssential oils are used as preservative in food industry and high concentration of essential oil is associated with negative organoleptic characteristics. This study presented a novel approach for synergistic antifungal effects by using the combination of thyme essential oil and Lactobacillus plantarum cell-free supernatant (LCFS) against Penicillium spp. and systematic evaluation of the antifungal effect by using fractional inhibitory concentration (FIC) index method. This approach will be a role model for future research on synergism and overcome the major drawbacks of using live bacteria and the negative effects arising from antimicrobial activities of essential oils.
“…For instance, to inhibit spoilage in food matrices, the addition of high concentration of antimicrobial agents is required, which oft en exceeds the acceptable fl avour threshold of consumers (5).…”
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