Combination of glycosphingosomes and liposomal doxorubicin shows increased activity against dimethyl-α-benzanthracene-induced fibrosarcoma in mice

Abstract

The present study aimed to assess the antitumor effect of glycosphingolipid-incorporated liposomes (glycosphingosomes) in combination with liposomal doxorubicin (Lip-Dox) in a mouse model of fibrosarcoma. Glycosphingosomes were prepared by incorporating glycosphingolipids isolated from Sphingomonas paucimobilis into the liposomes of 1,2-dipalmitoyl- sn -glycero-3-phosphocholine, cholesterol, and cardiolipin. Tumors were induced by administering dimethyl-α-benzanthracene, and tumor-bearing mice were treated with various formulations of Dox, including free Dox, Lip-Dox, or glycosphingosomes + Lip-Dox. Mice were observed for 90 days to monitor their survival and tumor size. Free Dox, but not Lip-Dox or a combination of glycosphingosomes and Lip-Dox, caused the substantial depletion of leukocytes and significantly increased the levels of lactate dehydrogenase and creatinine kinase in mice. Tumor-bearing mice treated with a combination of glycosphingosomes and Lip-Dox showed restricted tumor growth and increased survival when compared to those treated with free Dox or Lip-Dox. The results of the present study suggest that a combination of glycosphingosomes and Lip-Dox may prove to be very effective in the treatment of tumors.

Novel Photo- and Thermo-Responsive Nanocomposite Hydrogels Based on Functionalized rGO and Modified SIS/Chitosan Polymers for Localized Treatment of Malignant Cutaneous Melanoma

“…Pristine and functionalized GO (i.e., GO, GO-v50, GO-v50-DOX) presented a non-hemolytic behavior, as evidenced by hemolytic activities below 2% for all the studied concentrations ( Figure 10A ). As free DOX has been reported to yield a stronger hemolytic tendency, these results suggest it remains inactive while conjugated to GO ( Khan et al, 2015 ). Similarly, the evaluated hydrogels (S2C1 and S2C1GO) and their controls (S2 and C1) yielded hemolytic activities below 5% ( Figure 10B ).…”

Novel roles of PIWI proteins and PIWI-interacting RNAs in human health and diseases

“…Fibrosarcoma represents only a tiny percentage of all soft tissue sarcomas [ 344 ]. Currently, the treatment of metastatic fibrosarcoma mainly relies on chemotherapy [ 258 , 345 ]. piR-39,980 is downregulated in fibrosarcoma cells, causing doxorubicin resistance.…”

Liposome-Mediated Delivery of MERS Antigen Induces Potent Humoral and Cell-Mediated Immune Response in Mice

“…Blood was taken from mice of each group on day 5 after the final booster dose of immunization. In order to determine the safety of immunization, we analyzed the levels of the serum alanine transaminase (ALT), aspartate transaminase (AST), blood urea nitrogen (BUN), and lactate dehydrogenase (LDH) in the blood of control and immunized mice [23,24].…”