Combination of exogenous cell transplantation and 5-HT4 receptor agonism induce endogenous enteric neural crest-derived cells in a rat hypoganglionosis model

Yu, Hui; Zheng, Bin; Pan, Weikang; Wang, Huai-Jie; Xie, Chong; Zhao, Yuying; Chen, Xinlin; Liu, Yong; Gao, Ya

doi:10.1016/j.yexcr.2016.12.022

Cited by 13 publications

(15 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In addition, Sox10 staining can also be used in other areas of NCSCs, such as neural tracing or NCSC transplantation. Currently, p75NTR is commonly used to mark NCSCs in relevant transplantation treatment studies [ 34 , 35 ]. However, p75NTR is not only expressed in NCSCs but also continuously expressed in neurons derived from NCSC differentiation, which has a certain degree of interference to various assessments.…”

Section: Discussionmentioning

confidence: 99%

Sox10 Is a Specific Biomarker for Neural Crest Stem Cells in Immunohistochemical Staining in Wistar Rats

Yang

Huang

et al. 2020

Disease Markers

View full text Add to dashboard Cite

Objective. Neural crest stem cells (NCSCs) are prototypically migratory cells immigrating from the dorsal neural tube to specific embryonic sites where they generate a variety of cell types. A lot of biomarkers for NCSCs have been identified. However, which biomarkers are the most specific is still unclear. Methods. The rat embryos harvested in embryonic day 9 (E9), E9.5, E10, E10.5, E11, E12, E13, and E14 were paraffin-embedded and sectioned in transverse. NCSCs were spatiotemporally demonstrated by immunohistochemical staining with RET, p75NTR, Pax7, and Sox10. NCSCs were isolated, cultured, and stained with RET, p75NTR, Pax7, and Sox10. Results. In the paraffin sections of rat embryos, the immunohistochemical staining of RET, p75NTR, and Sox10 can all be used in demonstrating NCSCs. Sox10 was positive mainly in NCSCs while RET and p75NTR were positive not only in NCSCs but also in other tissue cells. In primary culture cells, Sox10 was mainly in the nucleus of NCSCs, RET was mainly in the membrane, and p75NTR was positive in cytoplasm and membrane. Conclusions. Sox10 is the specific marker for immunohistochemical staining of NCSCs in paraffin sections. In cultured cells, Sox10, p75NTR, and RET presented a similar staining effect.

show abstract

Section: Discussionmentioning

confidence: 99%

Sox10 Is a Specific Biomarker for Neural Crest Stem Cells in Immunohistochemical Staining in Wistar Rats

Yang

Huang

et al. 2020

Disease Markers

View full text Add to dashboard Cite

show abstract

“…In addition to its effects during development, 5-HT 4 agonism may also induce neurogenesis postnatally; neuron proliferation and differentiation increase significantly in postnatal gut-derived enteric neural stem/progenitor cells and colon explants cultured with 5-HT 4 receptor agonist (RS67506)-loaded liposomal nanoparticles, as well as in vivo (57). Similarly, ENCDC proliferation and differentiation were induced in a murine model of hypoganglionosis after 5-HT 4 agonist administration (138). These pro-neurogenic effects of 5-HT 4 agonism may, in part, result from its ability to shield neurons against toxic oxidative stress (12).…”

Section: Role Of Serotonin In Neurogenesis and Gi Motilitymentioning

confidence: 99%

Novel aspects of enteric serotonergic signaling in health and brain-gut disease

Colle

Israelyan

Margolis

2020

American Journal of Physiology-Gastrointestinal and Liver Physi

View full text Add to dashboard Cite

Gastrointestinal (GI) comorbidities are common in individuals with mood and behavioral dysfunction. Similarly, patients with GI problems more commonly suffer from co-morbid psychiatric diagnoses. Although the central and enteric nervous systems (CNS and ENS, respectively) have largely been studied separately, there is emerging interest in factors that may contribute to disease states involving both systems. There is strong evidence to suggest that serotonin may be an important contributor to these brain-gut conditions. Serotonin has long been recognized for its critical functions in CNS development and function. The majority of the body’s serotonin, however, is produced in the GI tract, where it plays key roles in ENS development and function. Further understanding of the specific impact that enteric serotonin has on brain-gut disease may lay the foundation for the creation of novel therapeutic targets. This review summarizes the current data focusing on the important roles that serotonin plays in ENS development and motility, with a focus on novel aspects of serotonergic signaling in medical conditions in which CNS and ENS co-morbidities are common, including autism spectrum disorders and depression.

show abstract

“…All surgical procedures used in this study had been previously described. 22 In summary, the procedure is as follows. Preoperative fasting was performed for 8 hours.…”

Section: Establishment Of the Rat Hypoganglionic Modelmentioning

confidence: 99%

Inhibition of ROCK signaling pathway accelerates enteric neural crest cell‐based therapy after transplantation in a rat hypoganglionic model

Zhao

et al. 2020

Neurogastroenterology Motil

Self Cite

View full text Add to dashboard Cite

Background Hirschsprung's disease (HSCR) is a congenital gastrointestinal disorder, characterized by enteric ganglia absence in part or entire of the colon, due to abnormal colonization and migration of enteric neural crest cells (ENCCs) during development. Currently, besides surgery which is the main therapy for HSCR, the potential of stem cell‐based transplantation was investigated as an alternative option. Although promising, it has limitations, including poor survival, differentiation, and migration of the grafted cells. We hypothesized that modulation of extracellular factors during transplantation could promote ENCCs proliferation and migration, leading to increased transplantation efficiency. Considering that the RhoA/ROCK pathway is highly involved in cytoskeletal dynamics and neurite growth, our study explored the effect of inhibition of this pathway to improve the success of ENCCs transplantation. Methods Enteric neural crest cells were isolated from rat embryos and labeled with a GFP‐tag. Cell viability, apoptosis, differentiation, and migration assays were performed with and without RhoA/ROCK inhibition. Labeled ENCCs were transplanted into the muscle layer of an induced hypoganglionic rat model followed by intraperitoneal injections of ROCK inhibitor. The transplanted segments were collected 3 weeks after for histological analysis. Key Results Our results showed that inhibition of ROCK increased viable cell number, differentiation, and migration of ENCCs in vitro. Moreover, transplantation of labeled ENCCs into the hypoganglionic model showed enhanced distribution of grafted ENCCs, upon treatment with ROCK inhibitor. Conclusions and Inferences ROCK inhibitors influence ENCCs growth and migration in vitro and in vivo, and should be considered to improve the efficiency of ENCCs transplantation.

show abstract

Combination of exogenous cell transplantation and 5-HT4 receptor agonism induce endogenous enteric neural crest-derived cells in a rat hypoganglionosis model

Cited by 13 publications

References 30 publications

Sox10 Is a Specific Biomarker for Neural Crest Stem Cells in Immunohistochemical Staining in Wistar Rats

Sox10 Is a Specific Biomarker for Neural Crest Stem Cells in Immunohistochemical Staining in Wistar Rats

Novel aspects of enteric serotonergic signaling in health and brain-gut disease

Inhibition of ROCK signaling pathway accelerates enteric neural crest cell‐based therapy after transplantation in a rat hypoganglionic model

Contact Info

Product

Resources

About