Combination of antimicrobials as an approach to reduce their application in aquaculture: Emphasis on the use of thiamphenicol/florfenicol against Aeromonas hydrophila

Assane, Inácio Mateus; Gozi, Kátia Suemi; Valladão, Gustavo Moraes Ramos

doi:10.1016/j.aquaculture.2019.04.021

Cited by 41 publications

(22 citation statements)

References 55 publications

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“…One of the most widely used strategies for treating and preventing bacterial diseases in aquaculture is the use of antibiotics (Assane, Gozi, Ramos Valladao, & Pilarski, 2019). However, the extensive use of different antibiotics has led to the development of multidrug resistance among various bacterial pathogens in aquaculture.…”

Section: Discussionmentioning

confidence: 99%

Emerging pathogens caused disease and mortality in freshwater mussels,Hyriopsis cumingii,in China

Yang

et al. 2020

Aquac Res

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In August 2019, massive mortalities of cultured Hyriopsis cumingii occurred in a local shellfish breeding experimental base in Chongqing, China. In this report, we identified 14 isolates from cultured mussels in mortality events and demonstrated that these strains caused mortality from 9% to 97%. Significant mussel mortality (p < .05) was observed in association with most isolates after bacterial challenge; however, considerable variation in the virulence of different isolates was noted, with survival ranging from 3% to 88% after 7 days. The most virulent strains (HOP3 and GL1) in haemolymph and tissues were identified as Stenotrophomonas maltophilia and Aeromonas veronii based on biochemical and 16S rDNA sequence analysis. The LD50 values of HOP3 and GL1 were found to be 2.45 × 106 CFU/g (body weight) and 2.24 × 105 CFU/g (body weight) respectively. Histopathology of H. cumingii infected with S. maltophilia and A. veronii displayed degeneration of muscle fibres and gill necrosis. We tested the antibiotic susceptibility of the two isolates and found that HOP3 was multidrug‐resistant. Growth optimization study revealed that both isolates remained surviving across wide environmental ranges. Our data lay the foundation for the pathogenic mechanism of mussel bacterial diseases, and they also suggest that effective control measures are worth further investigation.

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Section: Discussionmentioning

confidence: 99%

Emerging pathogens caused disease and mortality in freshwater mussels,Hyriopsis cumingii,in China

Yang

et al. 2020

Aquac Res

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“…This is also supported by a previous investigation that reveals significantly lower tissue drug residues within periods as short as 1 day in most tissues of broiler chicken following FF + TAP administration (Rairat et al, 2019). It is further supported by Assane et al (2019) recently who found that an FF + TAP combination with FICI ≤ 0.75 in vitro is also effective in vivo against A. hydrophila in Nile tilapia when 63% less antibiotic was used, resulting in a survival rate of 86%. If these results can be extended to pigs, there would be reduced risk of toxicity and an increase in human food safety.…”

Section: Discussionmentioning

confidence: 65%

“…Nevertheless, due to the same reproducibility concern, it is also well practiced that for strains with FICI values slightly above 0.5, their synergism are further confirmed with more reliable methods such as time-kill assay (Bayer and Morrison, 1984; White et al, 1996). The combination of FF + TAP has been proven effective against A. hydrophila , P. multocida , S. aureus , S. hyicus , and S. suis in vitro , as well as in animal models against A. hydrophila , S. aureus , and P. multocida in fish, mouse and chicken, respectively (Wei et al, 2016a, b; Assane et al, 2019). The FF + TAP combination was also demonstrated effective against strains that were originally resistant (Wei et al, 2016a, b).…”

Section: Discussionmentioning

confidence: 99%

“…In addition, isolates with FICI value ≤ 0.625 was detected in vitro for isolates of P. multocida (10/23) isolated from pigs, ducks and geese, Streptococcus suis (2/13) and Staphylococcus hyicus (1/6) isolated from pigs, as well as in vivo synergism against P. multocida in chickens (Wei et al, 2016b). A recent publication demonstrated in vitro FF and TAP with FICI ≤ 0.75 and in vivo efficacy at reduced dosage against Aeromonas hydrophila in Nile tilapia (Assane et al, 2019). While these results indicate preferential synergism by FF + TAP combination against certain bacterial species from pigs and fish and P. multocida from chickens, it remains unclear whether the synergistic actions extend to P. multocida or other major respiratory pathogens in pigs.…”

Section: Introductionmentioning

confidence: 99%

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In vitro and in vivo Synergistic Effects of Florfenicol and Thiamphenicol in Combination Against Swine Actinobacillus pleuropneumoniae and Pasteurella multocida

et al. 2019

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Potential synergism between florfenicol (FF) and thiamphenicol (TAP) was investigated for in vitro efficacy against Actinobacillus pleuropneumoniae and/or Pasteurella multocida as well as in vivo efficacy in swine. Among isolates of A. pleuropneumoniae (n = 58) and P. multocida (n = 79) from pigs in Taiwan that were tested, high percentages showed resistance to FF (52 and 53%, respectively) and TAP (57 and 53%, respectively). Checkerboard microdilution assay indicated that synergism [fractional inhibitory concentration index (FICI) ≤ 0.5] was detected in 17% of A. pleuropneumoniae (all serovar 1) and 24% of P. multocida isolates. After reconfirming the strains showing FICI ≤ 0.625 with time kill assay, the synergism increased to around 32% against both bacteria and the number could further increase to 40% against resistant A. pleuropneumoniae and 65% against susceptible P. multocida isolates. A challenge-treatment trial in pigs with P. multocida showed that the FF + TAP dosage at ratios correspondent to their MIC deduction was equally effective to the recommended dosages. Further on the combination, the resistant mutation frequency is very low when A. pleuropneumoniae is grown with FF + TAP and similar to the exposure to sub-inhibitory concentration of FF or TAP alone. The degree of minimum inhibitory concentration (MIC) reduction in FF could reach 75% (1/4 MIC) or more (up to 1/8 MIC for P. multocida, 1/16 for A. pleuropneumoniae) when combined with 1/4 MIC of TAP (or 1/8 for A. pleuropneumoniae). The synergism or FICI ≤ 0.625 of FF with oxytetracycline (47%), doxycycline (69%), and erythromycin (56%) was also evident, and worth further investigation for FF as a central modulator facilitating synergistic effects with these antimicrobials. Taken together, synergistic FF + TAP combination was effective against swine pulmonary isolates of A. pleuropneumoniae and P. multocida both in vitro and in vivo. Thus, this study may offer a potential alternative for the treatment of A. pleuropneumoniae and P. multocida infections and has the potential to greatly reduce drug residues and withdrawal time.

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“…Florfenicol (FFL), a fluorinated synthetic analog of thiamphenicol, is a broad-spectrum antimicrobial agent commonly used in veterinary medicine and aquaculture [11,12]. It binds to the 50S ribosomal subunit and inhibits protein synthesis.…”

Section: Introductionmentioning

confidence: 99%

Synergy between Florfenicol and Aminoglycosides against Multidrug-Resistant Escherichia coli Isolates from Livestock

et al. 2020

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The increasing prevalence of antimicrobial resistance and the laborious development of novel antimicrobial agents have limited the options for effective antimicrobial therapy. The combination of previously used antimicrobial agents represents an alternative therapy for multidrug-resistant (MDR) pathogens. The objective of this study was to investigate the synergistic effect of a florfenicol (FFL)-based combination with other antimicrobial agents against MDR Escherichia coli isolates from livestock using checkerboard assays and murine infection models. The FFL/amikacin (AMK) and FFL/gentamicin (GEN) combinations showed synergy against 10/11 and 6/11 MDR E. coli isolates in vitro, respectively. The combination of FFL with aminoglycosides (AMK or GEN) exhibited a better synergistic effect against MDR E. coli isolates than the cephalothin (CEF)/GEN or FFL/CEF combinations. The combination of FFL with AMK or GEN could reduce the emergence of resistant mutants in vitro. The FFL/AMK combination showed a higher survival rate of mice infected with MDR E. coli isolates than FFL or AMK alone. In summary, the combination of FFL with aminoglycosides (AMK or GEN) is highly effective against MDR E. coli isolates both in vitro and in vivo. Our findings may contribute to the discovery of an effective combination regimen against MDR E. coli infections in veterinary medicine.

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Combination of antimicrobials as an approach to reduce their application in aquaculture: Emphasis on the use of thiamphenicol/florfenicol against Aeromonas hydrophila

Cited by 41 publications

References 55 publications

Emerging pathogens caused disease and mortality in freshwater mussels,Hyriopsis cumingii,in China

Emerging pathogens caused disease and mortality in freshwater mussels,Hyriopsis cumingii,in China

In vitro and in vivo Synergistic Effects of Florfenicol and Thiamphenicol in Combination Against Swine Actinobacillus pleuropneumoniae and Pasteurella multocida

Synergy between Florfenicol and Aminoglycosides against Multidrug-Resistant Escherichia coli Isolates from Livestock

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