Combination of anti-tuberculosis drugs with vitamin C or NAC against different Staphylococcus aureus and Mycobacterium tuberculosis strains

Khameneh, Bahman; Bazzaz, Bibi Sedigheh Fazly; Amani, Alireza; Rostami, Javad; Vahdati-Mashhadian, Nasser

doi:10.1016/j.micpath.2015.11.006

Cited by 43 publications

(43 citation statements)

References 33 publications

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“…Our results [27] suggest that vitamin C can be used as an effective pre-treatment, to render CAP treatments more effective. This biofilmdestabilizing effects probably also accounts for previously reported effect of vitamin C in enhancing common antibiotic treatments, even against bacteria which are not directly killed by vitamin C [25,26]. This suggests that vitamin C could be used as a biofilm destabilizer, to either enhance the efficiently or reduce the exposure time required in antibacterial therapy.…”

Section: Combining Vitamin C With Cold Atmospheric Plasmasupporting

confidence: 52%

“…Vitamin C has been reported to be extremely toxic to Mycobacterium tuberculosis [24], but not to other common pathogens. There have been reports where vitamin C can be successfully used to enhance the effect of standard antibiotic treatments [25,26]. Our group has examined the effect of vitamin C on bacterial biofilms of that are not killed by vitamin C. We have found that vitamin C destabilizes their biofilms by inhibiting the production of the matrix EPS (Pandit & Mijakovic, unpublished results).…”

Section: Combining Vitamin C With Cold Atmospheric Plasmamentioning

confidence: 98%

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Combination of Cold Atmospheric Plasma and Vitamin C Effectively Disrupts Bacterial Biofilms

Pandit¹,

Mokkapati²,

Helgadottir³

et al. 2017

Clin Microbiol

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Cold atmospheric plasma (CAP) is increasingly used in medical applications for eradication of bacterial and tumor cells. CAP treatment devices, known as plasma jet pens, produce reactive oxygen and nitrogen species at atmospheric pressure and room temperature. The produced reactive species are concentrated in a small and precisely defined area, allowing for high precision medical treatments. CAP has been demonstrated as very effective against planktonic bacterial cells. Unfortunately, bacterial cells in biofilms are typically aggregated and protected by dense exopolymeric matrix, synthesized and secreted by the bacterial community. The main limitation in using CAP against bacterial biofilms is the thick protective matrix of extracellular polymers that shields bacterial cells within this complex architecture. CAP has also been shown to effectively eradicate tumor cells, but the main current limitation is the susceptibility of the surrounding healthy tissues to higher doses. We have recently demonstrated that vitamin C, a natural food supplement, can be used to destabilize bacterial biofilms and render them more susceptible to the CAP killing treatment. Here we discuss the possible impact that a pre-treatment with vitamin C could have on CAP applications in medicine. Specifically, we argue that vitamin C could enhance the effectiveness of CAP treatments against both the bacterial biofilms and some selected tumors.

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Section: Combining Vitamin C With Cold Atmospheric Plasmasupporting

confidence: 52%

Section: Combining Vitamin C With Cold Atmospheric Plasmamentioning

confidence: 98%

Combination of Cold Atmospheric Plasma and Vitamin C Effectively Disrupts Bacterial Biofilms

Pandit¹,

Mokkapati²,

Helgadottir³

et al. 2017

Clin Microbiol

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“…New generations of antibiotics, combination therapies, natural compounds, and drug delivery systems are mentioned as common approaches in this field [6, 7]. Therapy involving a combination of antibiotics shows several advantages; it enhances the antibiotic activity, prevents the emergence of resistance, and reduces the risk of infection [8].…”

Section: Introductionmentioning

confidence: 99%

Effect of Catechins, Green tea Extract and Methylxanthines in Combination with Gentamicin Against Staphylococcus aureus and Pseudomonas aeruginosa - Combination therapy against resistant bacteria -

Bazzaz¹,

Sarabandi²,

Khameneh³

et al. 2016

Journal of Pharmacopuncture

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Objectives:Bacterial resistant infections have become a global health challenge and threaten the society’s health. Thus, an urgent need exists to find ways to combat resistant pathogens. One promising approach to overcoming bacterial resistance is the use of herbal products. Green tea catechins, the major green tea polyphenols, show antimicrobial activity against resistant pathogens. The present study aimed to investigate the effect of catechins, green tea extract, and methylxanthines in combination with gentamicin against standard and clinical isolates of Staphylococcus aureus (S. aureus) and the standard strain of Pseudomonas aeruginosa (P. aeruginosa).Methods:The minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC) values of different agents against bacterial strains were determined. The interactions of green tea extract, epigallate catechin, epigallocatechin gallate, two types of methylxanthine, caffeine, and theophylline with gentamicin were studied in vitro by using a checkerboard method and calculating the fraction inhibitory concentration index (FICI).Results:The MICs of gentamicin against bacterial strains were in the range of 0.312 - 320 μg/mL. The MIC values of both types of catechins were 62.5 - 250 μg/ mL. Green tea extract showed insufficient antibacterial activity when used alone. Methylxanthines had no intrinsic inhibitory activity against any of the bacterial strains tested. When green tea extract and catechins were combined with gentamicin, the MIC values of gentamicin against the standard strains and a clinical isolate were reduced, and synergistic activities were observed (FICI < 1). A combination of caffeine with gentamicin did not alter the MIC values of gentamicin.Conclusion:The results of the present study revealed that green tea extract and catechins potentiated the antimicrobial action of gentamicin against some clinical isolates of S. aureus and standard P. aeruginosa strains. Therefore, combinations of gentamicin with these natural compounds might be a promising approach to combat microbial resistance.

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“…The antibacterial mechanism of NAC is thought to prevent the use of amino acid cysteine in bacteria or the reaction of sulfhydryl groups on NAC with proteins in bacterial cells. 21 NAC has a microbicidal effect and can inhibit the infection of the microorganism MTB by suppressing the host's oxidative response. NAC inhibits oxidative stress, lipid peroxidation, DNA oxidation, and cell death in macrophages infected with MTB.…”

Section: Nac In Tuberculosis a Nac As An Antioxidantmentioning

confidence: 99%

The Role of N-Acetyl Sistein in Pulmonary Tuberculosis

Yudhawati

Prasanta

2020

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Pulmonary tuberculosis is a chronic infection that is caused by Mycobacterium tuberculosis (MTB) infection and it isstill the major health problem worldwide. MTB infection can induce oxidative stress. Some studies have proved that active TB patients have an association with excessive oxidative stress which causes glutathione (GSH) levels to decrease and free radicals to increase. GSH facilitates the control of MTB intracellular bacterial growth in macrophages and has direct antimicrobial activity. N-acetyl cysteine (NAC) is a thiol, a precursor of L-cysteine and GSH that has been used for decades as a mucolytic agent in the treatment of respiratory diseases. Some studies have reported a beneficial role of NAC as an immunomodulator, besides, it also has anti-inflammatory and antimicrobial effect in TB management.

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Combination of anti-tuberculosis drugs with vitamin C or NAC against different Staphylococcus aureus and Mycobacterium tuberculosis strains

Cited by 43 publications

References 33 publications

Combination of Cold Atmospheric Plasma and Vitamin C Effectively Disrupts Bacterial Biofilms

Combination of Cold Atmospheric Plasma and Vitamin C Effectively Disrupts Bacterial Biofilms

Effect of Catechins, Green tea Extract and Methylxanthines in Combination with Gentamicin Against Staphylococcus aureus and Pseudomonas aeruginosa - Combination therapy against resistant bacteria -

The Role of N-Acetyl Sistein in Pulmonary Tuberculosis

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