Combination of AgNPs and Domiphen is Antimicrobial Against Biofilms of Common Pathogens

Abstract

Purpose The aim was to evaluate the antimicrobial potential of AgNPs synthesized with Artemisia argyi leaf extract and investigate the antimicrobial synergistic effects of AgNPs combined with domiphen and provide an efficient and broad-spectrum combination drug strategy. Methods AgNPs synthesized with Artemisia argyi leaf extract were studied using UV–vis spectroscopy, FTIR spectroscopy and particle size analysis. Then, Artemisia argyi leaf extract-synthesized AgNPs and domiphen were tested against Acinetobacter baumannii (ATCC 19606), Staphylococcus aureus (ATCC 6538), Escherichia coli (8099) and Candida albicans (ATCC 10231), respectively. Then, we explore synergistic antimicrobial effect and synergistic anti-biofilm effect through combined drug susceptibility test and combined drug minimum biofilm eradication concentration (MBEC 50 ) test. Results Characteristic absorption bands of AgNPs were found near 430 nm in the UV–vis spectrum. Particle size analysis results revealed that the average particle size of Artemisia argyi leaf extract-synthesized AgNPs was 77.6 nm. Artemisia argyi leaf extract-synthesized AgNPs showed high antimicrobial activity against the above four strains. Minimum inhibitory concentration (MIC) of Artemisia argyi leaf extract-synthesized AgNPs against strains was 1 μg/mL for Acinetobacter baumannii , 2 μg/mL for Staphylococcus aureus, Escherichia coli and Candida albicans . MBEC 50 of Artemisia argyi leaf extract-synthesized AgNPs against strains was 2 μg/mL for Acinetobacter baumannii , 4 μg/mL for Staphylococcus aureus , 1/2 μg/mL for Escherichia coli and 2 μg/mL for Candida albicans . The combination of Artemisia argyi leaf extract-synthesized AgNPs and domiphen has synergistic antimicrobial effect and synergistic anti-biofilm effect. Fractional inhibitory concentration (FIC) was ≤0.5. Conclusion Artemisia argyi leaf extract-synthesized AgNPs had antimicrobial activity against the above four strains. The combination of Artemisia argyi leaf extract-synthesized AgNPs and domiphen has synergistic antimicrobial effects to reduce the dosage of each antimicrobial drugs. Artemisia argyi leaf extract-synthesized AgNPs and domiphen have synergistic anti-biofilm effects.

Bioinspired Green Synthesis of Silver Nanoparticles Using Three Plant Extracts and Their Antibacterial Activity against Rice Bacterial Leaf Blight Pathogen Xanthomonas oryzae pv. oryzae

“…We explored the inhibition of Xoo strain C2 by three colloids of AgNPs in liquid [ 71 ]. In brief, 100 μL of three AgNPs colloids (10, 20, 40 μg/mL) were mixed with 100 μL Xoo strain C2 bacterial solution (OD 600 = 0.4), respectively, in the 96-well plate (Corning, NY, USA).…”

Rejuvenating the Activity of Usual Antibiotics on Resistant Gram-Negative Bacteria: Recent Issues and Perspectives

“…This has a high yield, ease of production of AgNPs, and a low cost, but the disadvantage is the use of chemicals harmful to human health [ 80 ]. Finally, the biological method consists of the use of reducing agents extracted from plants, such as the leaf of Artemisia argvi , which has a strong antioxidant activity and a biosynthesis potential for phenol and flavonoid compounds, which allows it to reduce Ag + to AgNPs [ 83 ]. The use of AgNPs in combination with an antibiotic has the advantage of reducing the necessary doses of the antibiotic, which reduces possible side effects [ 84 ].…”

Advances in the Application of Nanomaterials to the Treatment of Melanoma

“…In recent years, as the most commonly used metal NPs, AgNPs have received increasing attention, and their physicochemical properties, antibacterial activities, and toxicity to mammalian cells have been characterized [ 72 ]. Silver NPs (AgNPs) prepared and stabilized from various biologically active substances have a wide variety of biological and medical applications.…”