Combination Gene Transfection of TGF-<i>β</i>3 and BMP-2 Mediated by Lentivirus Induces Chondrogenic Differentiation of Rabbit Bone Marrow Mesenchymal Stem Cells

Xia, Changsuo; Li, Haiyan; Xuanying, Yang; Chenlei, Xia; Zhou, Yu; Hui, Huang

doi:10.1166/jbt.2018.1767

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2020

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“…The low transfection effectiveness also appears to be typical of the human chondrocytes since transfection above 40% has been reported with non-cationic lipids in bovine or rabbit chondrocytes [11,[13][14][15]. This explains why studies requiring a prolonged expression of a transgene in chondrocytes increasingly involve infections with retroviruses, adenoviruses, adeno-associated viruses, or more recently lentiviruses [16][17][18][19][20][21].…”

Section: Introductionmentioning

confidence: 99%

Nanoliposomes from Agro-Resources as Promising Delivery Systems for Chondrocytes

Bianchi

Velot

Kempf

et al. 2020

IJMS

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Investigations in cartilage biology have been hampered by the limited capacity of chondrocytes, especially in rats and humans, to be efficiently transfected. Liposomes are a promising delivery system due to their lipid bilayer structure similar to a biological membrane. Here we used natural rapeseed lecithin, which contains a high level of mono- and poly-unsaturated fatty acids, to evaluate the cytocompatibility of these phospholipids as future potential carriers of biomolecules in joint regenerative medicine. Results show that appropriate concentrations of nanoliposome rapeseed lecithin under 500 µg/mL were safe for chondrocytes and did not induce any alterations of their phenotype. Altogether, these results sustain that they could represent a novel natural carrier to deliver active substances into cartilage cells.

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