Nanoliposomes from Agro-Resources as Promising Delivery Systems for Chondrocytes

Bianchi, Arnaud; Velot, Émilie; Kempf, Hervé; Elkhoury, Kamil; Sánchez-González, Laura; Linder, Michel; Kahn, Cyril J.F.; Arab‐Tehrany, Elmira

doi:10.3390/ijms21103436

Cited by 10 publications

(17 citation statements)

References 47 publications

(52 reference statements)

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“…All these features allow liposomal nanoformulations to improve the drug therapeutic index and decrease its side effects [20]. Made of phospholipids, amphiphilic molecules commonly found in cells membrane and essential for its structure [21][22][23][24], liposomes can encapsulate and protect hydrophobic drugs in its membrane before fusion with the targeted cell membranes, and thus allowing the drug release at sufficient concentration [25]. Moreover, the surface of liposomes can also be functionalized to further increase its targeting efficiency [26,27].…”

Section: Curcumin Puritymentioning

confidence: 99%

Curcumin Loaded Nanoliposomes Localization by Nanoscale Characterization

Arab‐Tehrany

Elkhoury

Francius

et al. 2020

IJMS

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Curcumin is a hydrophobic drug gaining growing attention because of its high availability, its innocuity, and its anticancer, antitumoral, and antioxidative activity. However, its poor ‎‎bioavailability in the human body, caused by its low aqueous solubility and fast degradation, ‎‎presents a big hurdle for its oral administration. Here, we used nano-vesicles made of ‎‎phospholipids to carry and protect curcumin in its membrane. Various curcumin amounts were ‎‎encapsulated in the produced phospholipid system to form drug-loaded liposomes. ‎Curcumin’s ‎concentration was evaluated using UV-visible measurements. The maximal ‎amount of curcumin ‎that could be added to liposomes was assessed. Nuclear magnetic ‎resonance (NMR) analyses ‎were used to determine curcumin’s interactions and localization ‎within the phospholipid ‎membrane of the liposomes. X-ray scattering (SAXS) and atomic ‎force microscopy (AFM) ‎experiments were performed to characterize the membrane structure ‎and organization, as well as its ‎mechanical properties at the nanoscale. Conservation of the membrane’s properties is found with ‎the addition of curcumin in various ‎amounts before saturation, allowing the preparation of a ‎defined nanocarrier with desired ‎amounts of the drug.

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Section: Curcumin Puritymentioning

confidence: 99%

Curcumin Loaded Nanoliposomes Localization by Nanoscale Characterization

Arab‐Tehrany

Elkhoury

Francius

et al. 2020

IJMS

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“…Liposomes have been studied and investigated for more than five decades and have become a well-established drug delivery vesicle, resulting in the marketing authorization of many clinically approved liposome-based products to treat different diseases [ 1 ]. Liposomes’ resemblance to biomembranes enables superior biocompatibility and safety over other polymeric and metal-based nanoparticles, as well as the ability to deliver lipid-soluble and water-soluble molecules at the same time [ 2 , 3 ]. However, liposomes require surface modification with ligands to acquire smart targeting capabilities.…”

Section: Introductionmentioning

confidence: 99%

Engineering Smart Targeting Nanovesicles and Their Combination with Hydrogels for Controlled Drug Delivery

et al. 2020

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Smart engineered and naturally derived nanovesicles, capable of targeting specific tissues and cells and delivering bioactive molecules and drugs into them, are becoming important drug delivery systems. Liposomes stand out among different types of self-assembled nanovesicles, because of their amphiphilicity and non-toxic nature. By modifying their surfaces, liposomes can become stimulus-responsive, releasing their cargo on demand. Recently, the recognized role of exosomes in cell-cell communication and their ability to diffuse through tissues to find target cells have led to an increase in their usage as smart delivery systems. Moreover, engineering “smarter” delivery systems can be done by creating hybrid exosome-liposome nanocarriers via membrane fusion. These systems can be loaded in naturally derived hydrogels to achieve sustained and controlled drug delivery. Here, the focus is on evaluating the smart behavior of liposomes and exosomes, the fabrication of hybrid exosome-liposome nanovesicles, and the controlled delivery and routes of administration of a hydrogel matrix for drug delivery systems.

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“…Chondrocytes are surrounded by an extracellular matrix (ECM) they synthetize. Articular cartilage viscoelastic properties provide wear-resistant surfaces to the joint by improving the distribution of mechanical loads and moderating the friction between its surfaces (Sanchez-Adams et al, 2014;Lindahl, 2015;Bianchi et al, 2020;Masson and Krawetz, 2020). When injured or degenerating, articular cartilage is not able to restore its original organization because it has a very weak regenerative capacity.…”

Section: Introductionmentioning

confidence: 99%

Is Extracellular Vesicle-Based Therapy the Next Answer for Cartilage Regeneration?

Velot

Madry

Venkatesan

et al. 2021

Front. Bioeng. Biotechnol.

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“Extracellular vesicles” (EVs) is a term gathering biological particles released from cells that act as messengers for cell-to-cell communication. Like cells, EVs have a membrane with a lipid bilayer, but unlike these latter, they have no nucleus and consequently cannot replicate. Several EV subtypes (e.g., exosomes, microvesicles) are described in the literature. However, the remaining lack of consensus on their specific markers prevents sometimes the full knowledge of their biogenesis pathway, causing the authors to focus on their biological effects and not their origins. EV signals depend on their cargo, which can be naturally sourced or altered (e.g., cell engineering). The ability for regeneration of adult articular cartilage is limited because this avascular tissue is partly made of chondrocytes with a poor proliferation rate and migration capacity. Mesenchymal stem cells (MSCs) had been extensively used in numerous in vitro and preclinical animal models for cartilage regeneration, and it has been demonstrated that their therapeutic effects are due to paracrine mechanisms involving EVs. Hence, using MSC-derived EVs as cell-free therapy tools has become a new therapeutic approach to improve regenerative medicine. EV-based therapy seems to show similar cartilage regenerative potential compared with stem cell transplantation without the associated hindrances (e.g., chromosomal aberrations, immunogenicity). The aim of this short review is to take stock of occurring EV-based treatments for cartilage regeneration according to their healing effects. The article focuses on cartilage regeneration through various sources used to isolate EVs (mature or stem cells among others) and beneficial effects depending on cargos produced from natural or tuned EVs.

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Nanoliposomes from Agro-Resources as Promising Delivery Systems for Chondrocytes

Cited by 10 publications

References 47 publications

Curcumin Loaded Nanoliposomes Localization by Nanoscale Characterization

Curcumin Loaded Nanoliposomes Localization by Nanoscale Characterization

Engineering Smart Targeting Nanovesicles and Their Combination with Hydrogels for Controlled Drug Delivery

Is Extracellular Vesicle-Based Therapy the Next Answer for Cartilage Regeneration?

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