Column switching in zone electrophoresis on a chip

Kaniansky, Dušan; Masár, Marián; Danková, Mariana; Bodor, Róbert; Rákocyová, Renáta; Pilná, Michaela; Jöhnck, Matthias; Stanislawski, Bernd; Kajan, Slavomír

doi:10.1016/j.chroma.2004.08.065

Cited by 18 publications

(13 citation statements)

References 51 publications

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“…A combined CE system may be employed not only to additionally concentrate the analytes but also to remove a major part of the matrix stacker from the separation system. This was realized by Kaniansky et al [102], who applied a column-switching approach on chip for effectively controlling the destacking of anionic constituents (tartrate and citrate) during tITP-CE run.…”

Section: Combined Titp Systemsmentioning

confidence: 99%

Recent advances of transient isotachophoresis‐capillary electrophoresis in the analysis of small ions from high‐conductivity matrices

2006

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Since its invasion into the field of small ion analysis, quantitation of trace ionic analytes has always been a challenging task for CE, especially when dealing with highly saline samples. This review summarizes the method's progress and significant developments in the area due to its combination with on-line preconcentration by transient ITP (tITP). Principles of tITP stacking in high-conductivity solutions and operational preconcentration modes are considered. The most important application areas covered in this review include the analysis of seawater and biological fluids (urine, serum, etc.). Examples cited in this review demonstrate that tITP-CE is now on a certain way of becoming a recognized technique when the determination of trace ionic species in loaded matrices is the aim.

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Section: Combined Titp Systemsmentioning

confidence: 99%

Recent advances of transient isotachophoresis‐capillary electrophoresis in the analysis of small ions from high‐conductivity matrices

2006

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“…Preconcentration and preseparation of bulk components can be achieved also by application of ITP as a preceding step of CZE analysis realized either in column coupling system [46] or as a transient ITP process, which is continuously converted into ZE mode [47,48]. Transient ITP contributed to 15-20-fold sensitivity enhancement in the determination of both reduced and oxidized forms of glutathione in the diabetic nephropathy patient's plasma [49].…”

Section: Preconcentration and Preseparationmentioning

confidence: 99%

Recent developments in capillary electrophoresis and capillary electrochromatography of peptides

2006

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The article gives a comprehensive review on the recent developments in the applications of high-performance capillary electromigration methods, zone electrophoresis, isotachophoresis, isoelectric focusing, affinity electrophoresis, electrokinetic chromatography, and electrochromatography, to analysis, preparation, and physicochemical characterization of peptides. The article presents new approaches to the theoretical description and experimental verification of electromigration behavior of peptides, covers the methodological aspects of capillary electroseparations of peptides, such as rational selection of separation conditions, sample preparation, suppression of peptide adsorption, new developments in individual separation modes, and new designs of detection systems. Several types of applications of capillary electromigration methods to peptide analysis are presented: conventional qualitative and quantitative analysis, purity control, determination in biomatrices, monitoring of chemical and enzymatical reactions and physical changes, amino acid and sequence analysis, and peptide mapping of proteins. Some examples of micropreparative peptide separations are given and capabilities of capillary electromigration techniques to provide important physicochemical characteristics of peptides are demonstrated.

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“…Preconcentration and preseparation of bulk components can be achieved also by application of ITP as a preceding step of CZE analysis realized either in column coupling system [65] or as a transient ITP (t-ITP) process which is continuously converted into CZE mode [66][67][68]. Selective enrichment and ultrasensitive identification of trace peptides in proteome analysis was achieved using t-ITP-CZE coupled with nano-ESI-MS [69].…”

Section: Preconcentration and Preseparationmentioning

confidence: 99%

Recent developments in CE and CEC of peptides

Kašička

2007

Electrophoresis

118

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The article brings a comprehensive survey of recent developments and applications of high-performance capillary electromigration methods, zone electrophoresis, ITP, IEF, affinity electrophoresis, EKC, and electrochromatography, to analysis, preparation, and physicochemical characterization of peptides. New approaches to the theoretical description and experimental verification of electromigration behavior of peptides and to methodology of their separations, such as sample preparation, adsorption suppression, and detection, are presented. Novel developments in individual CE and CEC modes are shown and several types of their applications to peptide analysis are presented: conventional qualitative and quantitative analysis, purity control, determination in biomatrices, monitoring of chemical and enzymatical reactions and physical changes, amino acid and sequence analysis, and peptide mapping of proteins. Some examples of micropreparative peptide separations are given and capabilities of CE and CEC techniques to provide important physicochemical characteristics of peptides are demonstrated.

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Column switching in zone electrophoresis on a chip

Cited by 18 publications

References 51 publications

Recent advances of transient isotachophoresis‐capillary electrophoresis in the analysis of small ions from high‐conductivity matrices

Recent advances of transient isotachophoresis‐capillary electrophoresis in the analysis of small ions from high‐conductivity matrices

Recent developments in capillary electrophoresis and capillary electrochromatography of peptides

Recent developments in CE and CEC of peptides

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