Column selection for pharmaceutical analyses based on a column classification using four test parameters

Development and optimization of the determination of pharmaceuticals in water samples by SPE and HPLC with diode-array detection

“…Optimization of chromatographic separation is usually based on the change of the mobile phase composition but in this work the stationary phase was changed. Today the market is full of the different columns and there are a lots of computer programs which, based on experimental data, could recommend the best column for the investigated analytes and a given mobile phase . Anyway, in this paper complex and expensive software/programs were not used for choosing the best column.…”

Evaluation of an International Pharmacopoeia method for the analysis of ritonavir by liquid chromatography

“…Therefore, it was decided to examine the separation on a set of 18 columns, available in our laboratory and which are at least either base-deactivated or end-capped (the latter were included to check their performance). The columns were chosen based on a column ranking system developed in our laboratory [7][8][9]. A list of columns examined in this study is shown in Table 1.…”

“…As in previous studies, three arbitrarily chosen ranges of F-values were distinguished for the columns in Table 1: F < 2, 2 < F < 6 and F > 6 [7][8][9][10][11][12].…”

“…Since no brand names are mentioned in the monograph, the suitability of a set of 18 columns, all complying the prescriptions of the monograph, was investigated towards the separation of RTV and its impurities. It was checked whether a relation could be made with the column classification system developed in our laboratory [7][8][9]. Such a study can also be useful for the LC analysis of other pharmaceuticals described in pharmacopoeial texts.…”

Assessment of column selection systems using Partial Least Squares

“…In application of Snyder and Dolan's [29][30][31][32][33] hydrophobic-subtraction model, the measure for column ranking was the column selectivity function, (F s ) [34] constructed out of five selectivity parameters [29], while in the KUL column selection system (CSS) introduced by Hoogmartens et al [2,[35][36][37][38][39][40][41] the measure was the F metric [38] based on four selectivity parameters.…”