Development and optimization of the determination of pharmaceuticals in water samples by SPE and HPLC with diode-array detection

Pavlović, Dragana Mutavdžić; Ašperger, Danijela; Tolić, Dijana; Babić, Sandra

doi:10.1002/jssc.201300486

Cited by 17 publications

(6 citation statements)

References 30 publications

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“…Cependant, il est difficile de comparer ces ré-sultats obtenus avec ceux obtenus dans la présente étude du fait de la différence de matrice [29,30].…”

Section: Discussionunclassified

Mise en place d’une méthode semi-quantitative en toxicologie clinique par UHPLC-PDA en utilisant une extraction SPE en micro-élution

2013

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(190-400 nm). Résultats : Les rendements d'extraction étaient satisfaisants de même que la précision (CV% répétabilité < 6,54 %). Les CV inter-essais étaient élevés (CV > 15 % pour certaines molécules) mais diminuaient lorsqu'ils étaient rapportés aux 2 étalons internes (flurazépam et prazépam). La semi-quantification s'est faite à l'aide de 2 facteurs de réponses et les LOD et LOQ ont été comparées aux valeurs thé-rapeutiques ou toxiques. Conclusion : La méthode décrite est simple et rapide. Les résultats ont montré la capacité de la cartouche Strata r -X à extraire des molécules appartenant à plusieurs classes pharmacologiques avec des propriétés physico-chimiques différentes.Mots clés : Screening, SPE, UHPLC-PDA, toxicologie clinique, micro-élution Abstract -Objectives: A semi-quantitative method in clinical toxicology was developed using an extraction with a micro-elution solid phase (SPE) followed by an analysis with an Ultra-High-Performance Liquid ChromatographyPhotodiode Array system (UHPLC-PDA). The study was performed on 122 medicinal compounds according to the recommendations of the NF EN ISO 15189. Methods: After testing different SPE cartridges, the Strata r -X cartridge was selected based on its better extraction yields. A manual extraction protocol from plasma was set up for 3 concentrations (0.1 mg/L, 1 mg/L and 10 mg/L) with methanol as the eluting solvent. UHPLC analysis was performed with a mobile phase gradient of ammonium acetate 5 mM/Acetonitrile (ACN) (85/15, v/v) at pH 3.8 ± 0.05 and ACN on a BEH column C18 1.7 μm, 2.1 mm × 150 mm. The flow rate was 0.45 mL/min and the analytes were detected by PDA (190−400 nm). Results: Extraction yields were satisfactory, as well as the precision (repeatability CV% < 6.54%).Inter-assay CVs were high (CV > 15% for a few molecules) but were less significant when relativized by the two internal standards (flurazepam and prazepam). The semi-quantification was achieved with the support of two endpoints, and LOD and LOQ values were compared with therapeutic or toxic values. Conclusion: The method described is simple

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“…Cependant, il est difficile de comparer ces ré-sultats obtenus avec ceux obtenus dans la présente étude du fait de la différence de matrice [29,30].…”

Section: Discussionunclassified

Mise en place d’une méthode semi-quantitative en toxicologie clinique par UHPLC-PDA en utilisant une extraction SPE en micro-élution

2013

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“…The validated method was subjected to a comparative analysis with other analytical methods [31][32][33][34][35][36]. enced methods.…”

Section: Application To Environmental and Industrial Water Samplesmentioning

confidence: 99%

The potential of three different adsorbents in solid‐phase extraction of antihistaminic and antimigraine drugs from water samples using ultra‐high‐performance liquid chromatography‐ultraviolet analysis

Jebali,

Labidi,

Sghaier

et al. 2023

Separation Science Plus

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In this study, eight pharmaceuticals belonging to two classes, antihistaminic and antimigraine, were considered. Ultra‐performance liquid chromatography‐diode array detection was set up and gradient elution was used. The mobile phase was composed of acetonitrile and trifluoroacetic acid solution. Chromatographic separation was carried out with a run time of 9 min. The preconcentration of eight studied solutes was realized by micro solid‐phase extraction. Multiwalled carbon nanotubes, cetyltrimethylammonium bromide‐titanium dioxide nanotubes, and C‐18 bonded silica solid‐phase extraction (SPE) sorbents were evaluated and compared concerning recoveries. The results of the optimization of factors affecting the extraction efficiency including sample volume, the elution solvent type, and its volume are reported. For validation of the analytical procedure, specificity, linearity, precision, accuracy, limit of detection, and limit of quantification were evaluated. The developed method was successfully applied to the analysis of wastewater treatment samples. The extraction recoveries of pharmaceutical substances were above 81.23% for the three SPE sorbents. The extraction method was selective and the detection and quantification limits were between 0.004–0.02 and 0.01–0.06 μg/L, respectively. All the results indicated the potential application of the three studied SPE sorbents for the extraction of selected pharmaceuticals from wastewater.

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“…This method provide a better selectivity of the SPE sorbent for putified compounds (He and Giusti,2011). So far this method was used in order to detect drugs in water and aslo urine samples and lately was applied for antocyanins putification from edible sources (Pavlovic et al, 2010; Bierman and Sanchez, 2010 He and Giusti, 2011).…”

Section: Mixed-mode Techniquementioning

confidence: 99%

High-purity Anthocyanins Isolation using Solid Phase Extraction Tehniques

2016

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Anthocyanins are water soluble pigments used as natural colorants which have been extendedly studied due to their beautiful colors, and more important, beneficial health potential. Published studies regarding anthocyanins health benefits have been performed using crude anthocyanin extracts from natural sources with no any data about the purity of the tested extract. The impurities from the crude extracts are mostly other phenolic compounds; which can also have biological proprieties. In this case the beneficial potential of crude extract is not attributed only to anthocyanin. Thus, data regarding anthocyanins purity are required for the biological research. Currently, most investigations on anthocyanins are focused on their stability, as well as their purification and identification. In this paper, the most recent techniques in the chemical investigation of the anthocyanins purification are summarized.

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Development and optimization of the determination of pharmaceuticals in water samples by SPE and HPLC with diode-array detection

Cited by 17 publications

References 30 publications

Mise en place d’une méthode semi-quantitative en toxicologie clinique par UHPLC-PDA en utilisant une extraction SPE en micro-élution

Mise en place d’une méthode semi-quantitative en toxicologie clinique par UHPLC-PDA en utilisant une extraction SPE en micro-élution

The potential of three different adsorbents in solid‐phase extraction of antihistaminic and antimigraine drugs from water samples using ultra‐high‐performance liquid chromatography‐ultraviolet analysis

High-purity Anthocyanins Isolation using Solid Phase Extraction Tehniques

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