Column efficiency in micellar electrokinetic capillary chromatography

Abstract

Experimental factors that Influence column efficiency In micellar electrokinetic capillary chromatography were studied.Parameters, such as applied voltage, column dimensions, and concentrations of buffer and surfactant In the mobile phase, are found to Influence efficiency. Van Deemter-llke plots of plate height vs. applied voltage are used to demonstrate the significance of factors which typically cause band dispersion In chromatography and electrophoresis. Dispersion due to resistance to mass transfer In the mobile phase and temperature gradients within the column are shown to be most significant. Plate heights of less than 10 µ are possible when experimental parameters are optimized.

Separation of peptides by strong cation-exchange capillary electrochromatography

“…But it was found experimentally that the stacking was not very effective in practice as shown in Fig. 3, which might be caused by the similar reason as reported in CE [32]. Since electroosmosis occurs much more rapidly in the diluted sample solution than the running mobile ing of buffer concentration in sample solutions from 10 to 40 mM KH PO buffers, the reason for which 2 4 (t 2 t ) strength of the sample buffer in CE [33], when the the migration time of a neutral and chromatographelectrokinetic injection with highly ionic strength of ically unretained solute.…”

Enantiomeric separation of neutral hydrophobic dihydrofuroflavones by cyclodextrin‐modified micellar capillary electrophoresis

“…Slow mass transfer between the micelle, CD, and bulk solution is a likely source of lower efficiencies, since the low aqueous solubility of the dihydrofuroflavones limits their transfer between the CD and micelle pseudophases and the bulk run buffer. Also, the high voltage used in this study produces high micelle velocities, inhibiting the ability of the analyte to exchange rapidly to the CD [26]. Furthermore, this voltage produces significant Joule heating.…”

Parameters controlling the elution window and retention factors in micellar electrokinetic capillary chromatography

“…Applying the same instrumentation as in capillary zone electrophoresis, in MECC uncharged compounds can be separated based on differences in their partitioning between two phases, just as in chromatographic techniques. Since the introduction of MECC by Terabe et al [1,2], several authors have paid attention to the fundamental characteristics of this separation method [3,4] and to the effect of different separation parameters on the migration behavior [5][6][7][8][9][10][11]. Also the theoret-the solute in the micellar and the aqueous phase, and ts, tEO F and tMC are the migration times of the solute, the electroosmotic flow (EOF) and the micelles, respectively.…”