Column and Thin-Layer Chromatographic Methods for the Simultaneous Determination of Acediasulfone in the Presence of Cinchocaine, and Cefuroxime in the Presence of Its Hydrolytic Degradation Products

Abstract

Column liquid chromatography (LC) and thin-layer chromatography (TLC)densitometry methods are described for simultaneous determination of acediasulfone (Ace) and cinchocaine (Cinco). In the LC method, the separation and quantitation of the 2 drugs was achieved on a Zorbax C8 column (5 μm, 150 × 4.6 mm id) using a mobile phase composed of methanol-phosphate buffer, pH 2.5 (66 + 34, v/v), at a flow rate of 1 mL/min and ultraviolet detection at 300 and 327 nm for Ace and Cinco, respectively. The method showed linearity over concentration ranges of 20-200 and 45685 μg/mL, respectively. In the TLCdensitometry method, a mobile phase composed of methanol-tetrahydrofuran-acetic acid (45 + 5 + 0.5, v/v/v) was used for the separation of the 2 drugs. The linearity range was 0.5-4 and 2-9 μg/spot, respectively. In addition, stability indicating TLCdensitometry method has been developed for determination of cefuroxime sodium in the presence of 570% of its known hydrolytic degradation products. The mobile phase butanol-methanol-tetrahydrofuran-concentrated ammonium hydroxide (50 + 50 + 50 + 5, v/v/v/v) was used. The concentration range was 210 g/spot. The optimized methods proved to be specific and accurate for the analysis of the cited drugs in laboratory-prepared mixtures and dosage forms. The obtained results agreed statistically with those obtained by the reference methods.

Electrochemical Detection of a Local Anesthetic Dibucaine at Arrays of Liquid|Liquid MicroInterfaces

“…DIC is commonly drochloride or a free base in diluting penetrable solutions to produce sp anesthesia or ophthalmic ointments to initiate conjunctival anesthesia. To date, many methods have been used to determine DIC, including high performance liquid chromatography (HPLC) [8][9][10][11], gas chromatography (GC) [12][13][14], column liquid chromatography (LC), thin-layer chromatography (TLC) [15], fluorimetry [16], spectrophotometry [17][18][19] and electrochemical methods [20][21][22][23]. However, most of the previous methods are associated with disadvantages such as being time-intensive, the requirement of sample pre-treatment, intensive solvent usage, the requirement of extraction and the use of expensive devices.…”