Colour Tests for the Detection and Characterisation of Tertiary Amines.

Groth, Arne B.; Wallerberg, Gun; Schroll, Gustav; Bowie, J. H.; Williams, D. H.; Bunnenberg, E.; Djerassi, Carl; Records, Ruth

doi:10.3891/acta.chem.scand.20-2628

Cited by 16 publications

(10 citation statements)

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“…However, it seems apparent that the final product was the result of a base‐catalyzed condensation reaction of MAA where the tertiary amine groups of the phenothiazine drugs acted as a catalyst. Groth and Wallerburg suggested a structure for MAA but they did not offer a definite identification about the final product (Fig. ).…”

Section: Resultsmentioning

confidence: 99%

Spectrofluorimetric determination of certain biologically active phenothiazines in commercial dosage forms and human plasma

et al. 2012

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A validated simple and sensitive spectrofluorimetric method was developed for the determination of chlorpromazine hydrochloride, promethazine hydrochloride, trifluperazine hydrochloride, thioridazine hydrochloride, perazine maleate and oxomemazine. The method was based on condensation of malonic acid/acetic anhydride (MAA) under the catalytic effect of the tertiary amine moiety of the studied phenothiazines to provide a deep yellow to brown colour with green florescence. Relative fluorescence intensity of the products was measured at λexc 398 nm and λem 432 nm. Different variables affecting the reaction were studied and optimized. The method was successfully applied for the determination of the studied drugs in commercial dosage forms. The lower detection limits allowed the application of this method for the determination of the compounds in plasma as an example of a biological fluid. In addition, the method was considered specific for the determination of tertiary amines in the presence of primary and secondary amines; as a result, it was deemed suitable for the determination of the cited drugs in the presence of their degradation products resulting from N‐dealkylation or oxidation of the corresponding sulphoxides or sulphones. Copyright © 2012 John Wiley & Sons, Ltd.

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Section: Resultsmentioning

confidence: 99%

Spectrofluorimetric determination of certain biologically active phenothiazines in commercial dosage forms and human plasma

et al. 2012

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“…It has been reported that the reactions between polycarboxylic acids containing active methylene moieties and acetic anhydride could be catalyzed by tertiary amines to give highly coloured and fluorescent products. 14,[22][23][24][25] Malonic acid -acetic anhydride mixture, as an example of these mixtures, has been reported for the fluorimetric determination of some alkaloids 14 and trimebtine. 25 In all the previous works, 14,[22][23][24][25] it was suggested that malonic acid and acetic anhydride reacted together under the catalytic effect of tertiary amines to give coloured product of highly relative fluorescence intensity.…”

Section: Spectrofluorimetric Methodsmentioning

confidence: 99%

Simple Spectrophotometric and Spectrofluorimetric Methods for Determination of Trimetazidine Dihydrochloride

Abdelmageed

2004

Bulletin of Pharmaceutical Sciences. Assiut

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Two simple and sensitive spectrophotometric and spectrofluorimetric methods have been described for the determination of trimetazidine dihydrochloride in bulk and dosage forms. The first method depends on ion pair complexation between the cited drug with two dyes; tropaeloin 000 and methyl orange in aqueous medium. All variables affecting the formation of complex were studied and optimized. The coloured products of the drug with tropaeloin 000 and methyl orange were measured at 490 nm and 422 nm respectively, after extraction with chloroform. Beer's law was obeyed in the concentration ranges 4-20 μg ml -1 and 2-12 μg ml -1 for tropaeloin 000 and methyl orange respectively. The spectrofluorimetric method depends on the condensation of malonic acid and acetic anhydride under the catalytic effect of trimetazidine dihydrochloride. The condensation product gave two emission maxima measured at 452 nm (λ ex 393 nm) and 470 nm (λ ex 428 nm). Variables affecting this condensation reaction were studied and optimized. At both maxima of emission good correlation was observed in the range 40-200 ng ml -1 . The proposed methods were applied successfully for the determination of the cited drug in tablet dosage form without interference from common encountered additives. Percentage recoveries ranged from 98.9% to 99.7% in bulk and 96.9% to 98.3% in tablet dosage forms analysis.

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“…Groth and Wallerberg [22] have postulated a structure of the mixed anhydride of malonic acid and acetic anhydride ( Figure 11). In the present work, it seems apparent that the final product results from base catalyzed condensation reaction of the mixed anhydride of malonic acid and acetic anhydride, where the tertiary amine groups of OMZ and CLM act as the basic catalyst ( Figure 11).…”

Section: The Reaction Mechanism For Omz and Clm Interaction With Malomentioning

confidence: 99%

Spectrodensitometric determination of some pharmaceutical mixtures containing proton pump inhibitors

El-Kommos

Khashaba

El-Wekil

2014

Journal of Planar Chromatography – Modern TLC

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SummaryTwo ultraviolet (UV)-spectrodensitometric methods were proposed for the separation and determination of binary and ternary mixtures containing proton pump inhibitors (PPIs). Method A was developed for the assay of omeprazole, panoprazole, rabeprazole, and lansoprazole in binary mixtures with domperidone (DOM) using their isoabsorptive points. Method B was developed for the determination of a ternary mixture containing omeprazole, tinidazole, and clarithromycin. It depends on using malonic acid-acetic anhydride as a spraying reagent to form yellow color (for omeprazole) and dark brown color (for clarithromycin) that are measured at λ max = 350 nm and λ max = 492 nm, respectively, while tinidazole was measured by UV at λ max = 310 nm. For method A, Beer's law was obeyed for all studied PPIs and domperidone in the concentration range of 180-1440 and 120-720 ng spot −1 , respectively. The detection limits for proton pump inhibitors and domperidone were 48.05-73.45 and 30.74-32.50 ng spot −1 , respectively, and the quantitation limits were 145.59-222.47 and 93.14-98.48 ng spot −1 , respectively. For method B, Beer's law was obeyed for omeprazole, clarithromycin, and tinidazole in the concentration range of 180-480, 2250-6000, and 30-180 ng spot −1 , respectively. The detection limits for omeprazole, clarithromycin, and tinidazole were 20.36, 272.60, and 2.05 ng spot −1 , respectively, and the quantitation limits were 61.7, 825.9, and 6.2 ng spot −1 , respectively, for the investigated drugs.

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Colour Tests for the Detection and Characterisation of Tertiary Amines.

Cited by 16 publications

References 0 publications

Spectrofluorimetric determination of certain biologically active phenothiazines in commercial dosage forms and human plasma

Spectrofluorimetric determination of certain biologically active phenothiazines in commercial dosage forms and human plasma

Simple Spectrophotometric and Spectrofluorimetric Methods for Determination of Trimetazidine Dihydrochloride

Spectrodensitometric determination of some pharmaceutical mixtures containing proton pump inhibitors

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