Spectrodensitometric determination of some pharmaceutical mixtures containing proton pump inhibitors

Abstract

SummaryTwo ultraviolet (UV)-spectrodensitometric methods were proposed for the separation and determination of binary and ternary mixtures containing proton pump inhibitors (PPIs). Method A was developed for the assay of omeprazole, panoprazole, rabeprazole, and lansoprazole in binary mixtures with domperidone (DOM) using their isoabsorptive points. Method B was developed for the determination of a ternary mixture containing omeprazole, tinidazole, and clarithromycin. It depends on using malonic acid-acetic anhydride as a spraying reagent to form yellow color (for omeprazole) and dark brown color (for clarithromycin) that are measured at λ max = 350 nm and λ max = 492 nm, respectively, while tinidazole was measured by UV at λ max = 310 nm. For method A, Beer's law was obeyed for all studied PPIs and domperidone in the concentration range of 180-1440 and 120-720 ng spot −1 , respectively. The detection limits for proton pump inhibitors and domperidone were 48.05-73.45 and 30.74-32.50 ng spot −1 , respectively, and the quantitation limits were 145.59-222.47 and 93.14-98.48 ng spot −1 , respectively. For method B, Beer's law was obeyed for omeprazole, clarithromycin, and tinidazole in the concentration range of 180-480, 2250-6000, and 30-180 ng spot −1 , respectively. The detection limits for omeprazole, clarithromycin, and tinidazole were 20.36, 272.60, and 2.05 ng spot −1 , respectively, and the quantitation limits were 61.7, 825.9, and 6.2 ng spot −1 , respectively, for the investigated drugs.

Complexation Based Voltammetric Determination of Pantoprazole Sodium in Pharmaceutical Formulations and Rabbit Plasma

“…Its efficacy as antiulcer and anti‐secretory agent has been well established. Several methods have been previously used for its determination in pharmaceutical dosage forms and biological fluids either alone or in combination with other co‐administered drugs including UV‐Visible spectrophotometry , spectrofluorimetry , high performance thin‐layer chromatography , high performance liquid chromatography ,, capillary electrophoresis and electrochemical methods .…”

Determination of omeprazole via fluorescence‐quenching methodology; application to content uniformity testing

“…Variable analytical methods have been previously performed for the determination of OMP, including ultraviolet-visible (UV-vis) spectrophotometry [3][4][5][6], spectrofluorimetry [7][8][9], electrochemical methods [10][11][12][13], electrophoresis [14][15][16], high-performance liquid chromatography (HPLC) [17][18][19][20][21], ultrahigh-performance liquid chromatography (UPLC) [22] and liquid chromatography-tandem mass spectrometry (LC-MS/MS) [23].…”

A New and Cost Effective Approach for Simultaneous Voltammetric Analysis of two Related Benzimidazole Drugs and their Determination in Biological Fluids

“…Sometimes, they are administered separately and commonly administered in a binary mixture form in capsules. Analytical literatures concerning the determination of such mixture include UV‐spectrophotometric methods , spectrodensitometry , HPLC method .…”