Colour change in the human histamine wheal made visible by LYYN: a technique to enhance colour differences

Holm, Olle; Lindell, Erik; Malm, Lars

doi:10.1111/j.1600-0846.2010.00457.x

Cited by 5 publications

(10 citation statements)

References 5 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…During a wheal reaction it has been shown that clinical photography with enhanced colour differences is more sensitive in detecting differences compared to ordinary photographs using colour quantification software. A peak in red colour is found after 8 minutes followed by a rapid decrease in red colour . Compared to our data in which the superficial vessels at 150 and 300 μm show a rapid increase in blood flow from baseline to 8 minutes, it may be implied, that the flare of histamine induced wheals reaches peak value after ≈8 minutes.…”

Section: Discussioncontrasting

confidence: 74%

“…A peak in red colour is found after 8 minutes followed by a rapid decrease in red colour. 24 Compared to our data in which the superficial vessels at 150 and 300 μm show a rapid increase in blood flow from baseline to 8 minutes, it may be implied, that the flare of histamine induced wheals reaches peak value after ≈8 minutes. The broadest field of view for investigating inflammation can be achieved by thermography.…”

Section: Quantification Of Wheal Reactions and Cutaneous Blood Flowcontrasting

confidence: 83%

See 1 more Smart Citation

Dynamic optical coherence tomography of histamine induced wheals

Olsen

Birch-Johansen

Themstrup

et al. 2018

Skin Research and Technology

View full text Add to dashboard Cite

show abstract

Section: Discussioncontrasting

confidence: 74%

Section: Quantification Of Wheal Reactions and Cutaneous Blood Flowcontrasting

confidence: 83%

Dynamic optical coherence tomography of histamine induced wheals

Olsen

Birch-Johansen

Themstrup

et al. 2018

Skin Research and Technology

View full text Add to dashboard Cite

show abstract

“…In ophthalmology, digital photography and analysis are used for diagnosis of the anterior eye segment [37], for vessel detection in corneal transplants [38], for analyzing the corneal involvement of pterygium conjunctivae [39,40], for noninvasive calculation of hemoglobin in the wide field of emergency medicine [41], for examining rotational stability of intraocular lenses [42], in diagnosis of dry eye [43] and in the observation of side effects of antiglaucomatous treatment [44]. In dermatology they are implemented for measuring erythema and edema of the skin prick test [45,46,47] or intradermal skin tests [48], for analyzing repigmentation during treatment of vitiligo [49], for quantification of skin lesions [50], and in diagnosis and treatment of atopic dermatitis [51,52]. Otorhinolaryngology makes use of this technique during endoscopic transsphenoidal pituitary surgery [53] and for video-laryngoscopic-assisted surgery [54], while it is used for analysis of body posture in patients with asthma [55,56] in pediatrics.…”

Section: Introductionmentioning

confidence: 99%

Objectifying the Conjunctival Provocation Test: Photography-Based Rating and Digital Analysis

Dogan

Astvatsatourov

Deserno

et al. 2013

Int Arch Allergy Immunol

View full text Add to dashboard Cite

Background: Patients with allergic rhinoconjunctivitis are susceptible to both nasal and ocular symptoms. The conjunctival provocation test (CPT) is an established diagnostic procedure used in allergic rhinoconjunctivitis, particularly to document a patient's current reactivity to allergens. To date, there are no international guidelines defining the CPT. No approved evaluation method exists for interpreting CPT results. This paper aims to establish the digital analysis of macroimages as an objective, validated and standardized method for interpreting CPT results. Methods: In a clinical immunotherapy trial with 155 patients, treatment progress was documented based on the CPT. Local investigators used a symptom score to grade tearing, reddening and the patients' subjective perception of symptoms (mucosal irritation). A central observer rated conjunctival hyperemia via digital photography. Digital image analysis software was utilized to determine conjunctival hyperemia. Results: Spearman's correlation between the local investigators' and the central observer's ratings was r = 0.729 (p < 0.001); the percentage of total agreement was 48% (based on 739 photos). Digital image analysis (based on 48 photos) had a high percentage of total agreement with the central observer's ratings (69%) but a low percentage of total agreement with the investigators' ratings (38%). The corresponding correlations were r = 0.264 and 0.064, respectively. Conclusion: Photography-based rating by a central observer may represent a valuable supplement to the local investigator's assessment for making an objective evaluation of CPT results. Digital image analysis possesses the potential of being an objective evaluation method compared to the wide-spread subjective evaluation by the investigators.

show abstract

“…In a study of medical students who were skin prick tested with histamine, we detected a colour shift of the wheals from pale red to darker red between the 18th and the 20th minutes after the skin prick test (SPT) . One suggestion to explain the colour shift found was total desensitization of histamine H1 receptors (H1 receptors) in turn causing change of post‐capillary resistance vessels from vasoconstriction to vasodilatation.…”

Section: Introductionmentioning

confidence: 99%

Re‐sensitization of desensitized histamine H1 receptors in the human skin takes more than 18 hours

Malm

2019

Skin Research and Technology

Self Cite

View full text Add to dashboard Cite

Background: It is known since the time of testing histamine on pieces of guinea pig's jejunum that histamine receptors can develop insensitivity. The aim was to find evidence for desensitization of histamine H1 receptors in the human skin in vivo and, if found, to study the time for such receptors to regain normal sensitivity. Materials and Methods: A skin prick test with histamine (10 mg mL-1) was set in areas where a large histamine wheal was evoked 2, 6, 18, 24 or 72 hours earlier. A skin prick test with histamine (10 mg mL-1) was also set in an area where an allergen wheal was evoked 2 or 6 hours earlier. Heights, diameters and areas were measured on photographs of side views of plaster casts of the evoked skin elevations. Results: Histamine wheals, called test wheals, in areas where large histamine wheals were evoked 2, 6 or 18 hours earlier, were smaller than histamine wheals, called initial wheals, in earlier non-stimulated areas. Test wheals from the 18 hours experiment were smaller than test wheals from the 72 hours experiment. Test wheals evoked in areas where allergen wheals were evoked 2 or 6 hours earlier were smaller than corresponding initial wheals. Conclusion: Histamine-evoked wheals and IgE-mediated allergic wheals reduce the sensitivity of histamine H1 receptors in the human skin. It takes between 18 and 72 hours to restore the sensitivity. Similarities between the development of histamine wheals in the human skin and histaminergic migraine with aura are discussed.

show abstract

Colour change in the human histamine wheal made visible by LYYN: a technique to enhance colour differences

Abstract: The LYYN technique was sensitive enough to discover a rapid colour change in the histamine wheal, a change that has not been described before.

Cited by 5 publications

References 5 publications

Dynamic optical coherence tomography of histamine induced wheals

Dynamic optical coherence tomography of histamine induced wheals

Objectifying the Conjunctival Provocation Test: Photography-Based Rating and Digital Analysis

Re‐sensitization of desensitized histamine H1 receptors in the human skin takes more than 18 hours

Contact Info

Product

Resources

About