Colorimetric point-of-care paper-based sensors for urinary creatinine with smartphone readout

Lewińska, Izabela; Speichert, Mikołaj; Granica, Mateusz; Tymecki, Łukasz

doi:10.1016/j.snb.2021.129915

Cited by 69 publications

(31 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The smartphone was modified with 3-D-printed elements to ensure repeatable placement of the sensor as well as to limit the influence of outside lightning conditions on the obtained results. Such details of smartphone modification have already been shown elsewhere [ 17 ]. Briefly, the sensors were illuminated from behind with the smartphone’s flashlight, guided by aluminum foil.…”

Section: Methodsmentioning

confidence: 84%

“…For the determination of two different analytes, namely protein and creatinine, on a single paper strip, it was necessary to search for conditions allowing for sufficiently sensitive determination of both compounds. Creatinine was determined according to a previously established protocol [ 17 ]: 0.3 mol·L −1 3,5-dinitrobenzoic acid in 0.45 mol·L −1 sodium hydroxide was deposited in the sampling channel and 2 mol·L −1 sodium hydroxide was pipetted on the detection zone. In a previous study, we established that this placement of reagents allows for uniform color development in the detection zone.…”

Section: Resultsmentioning

confidence: 99%

“…Additionally, lamination allowed less grainy photos of the detection zone to be obtained (otherwise, the roughness of paper could be seen in the photos). µPADs for protein to creatinine ratio determination were cut out and folded following the line between the blue and black region (see Figure 1 B) prior to lamination, similarly to our earlier work [ 17 ]. Then, they were placed between two laminating films and passed through an office laminator.…”

Section: Methodsmentioning

confidence: 99%

“…Then, they were placed in the smartphone case and a photo was taken. For more detail on the analytical procedure, refer to [ 17 ] and Figure S1 (steps E–H). Unless stated otherwise, each measurement was conducted in triplicate.…”

Section: Methodsmentioning

confidence: 99%

“…Owing to a careful condition optimization, the obtained analytical parameters for protein determination methods significantly exceed the ones presented in [ 14 ]. The best performing protein assay was then used for protein measurement to determine the protein to creatinine ratio while creatinine was quantified according to our previous study [ 17 ] with 3,5-dinitrobenzoic acid. Signal acquisition was accomplished with an appropriately modified smartphone to further increase the potential of the developed sensors as point-of-care tests, which makes the presented research the first smartphone-assisted PCR quantitative analysis.…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Smartphone-Assisted Protein to Creatinine Ratio Determination on a Single Paper-Based Analytical Device

Lewińska¹,

Kurdziałek²,

Tymecki³

2021

Molecules

Self Cite

View full text Add to dashboard Cite

Proteinuria is a condition in which an excessive amount of protein is excreted in urine. It is, among others, an indicator of kidney disease or risk of cardiovascular disease. Rapid and reliable diagnosis and monitoring of proteinuria is of great importance for both patients and their physicians. For that reason, a paper-based sensor for proteinuria diagnosis was designed, optimized, and validated utilizing smartphone-assisted signal acquisition. In the first step, a few commonly employed protein assays were optimized and compared in terms of analytical performance on paper matrix. The tetrabromophenol blue method was selected as the one providing a sufficiently low limit of detection (39 mg·L−1) on the one hand and appropriate long-term stability (up to 3 months) on the other hand. The optimized assay was employed for protein-to-creatinine ratio (PCR) determination on a single paper-based sensor. For both analytes the linear ranges were within the clinically relevant range. The analytical usefulness of the developed sensors was demonstrated by a PCR recovery study in artificial urine. The obtained PCR recoveries were from ca. 80 to 150%.

show abstract

Section: Methodsmentioning

confidence: 84%

Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Smartphone-Assisted Protein to Creatinine Ratio Determination on a Single Paper-Based Analytical Device

Lewińska¹,

Kurdziałek²,

Tymecki³

2021

Molecules

Self Cite

View full text Add to dashboard Cite

show abstract

A Point‐of‐Care Sensing Platform for Multiplexed Detection of Chronic Kidney Disease Biomarkers Using Molecularly Imprinted Polymers

Li,

Luo,

Kong

et al. 2024

Adv Funct Materials

View full text Add to dashboard Cite

Chronic kidney disease (CKD) is one of the most serious non‐communicable diseases affecting the population. In the early‐stages patients have no obvious symptoms, until it becomes life‐threatening leading end‐stage kidney failure. Therefore, it is important to early diagnose CKD to allow therapeutic interventions and progression monitoring. Here, a point‐of‐care (POC) sensing platform is reported for the simultaneous detection of three CKD biomarkers, namely creatinine, urea, and human serum albumin (HSA), using reduced graphene oxide/polydopamine‐molecularly imprinted polymer (rGO/PDA‐MIP) fabricated with novel surface‐molecularly imprinting technology. A multi‐channel electrochemical POC readout system with differential pulse voltammetry (DPV) function is developed, allowing the simultaneous detection of the three biomarkers, in combination with the surface‐MIP electrodes. This sensing platform achieves the record low limit‐of‐detection (LoD) at a femtomolar level for all three analytes, with wide detection ranges covering their physiological concentrations. Clinical validation is performed by measuring these analytes in serum and urine from healthy controls and patients with CKD. The average recovery rate is 81.8–119.1% compared to the results obtained from the hospital, while this platform is more cost‐effective, user‐friendly, and requires less sample‐to‐result time, showing the potential to be deployed in resource‐limited settings for the early diagnosis and tracking progression of CKD.

show abstract

A turn off–on fluorometric and paper‐based colorimetric dual‐mode sensor for isoniazid detection

2021

View full text Add to dashboard Cite

In the present study, cobalt oxyhydroxide (CoOOH) nanosheets were applied for establishing a dual fluorometric and smartphone‐paper‐based colorimetric method to detect isoniazid. CoOOH nanosheets quenched the fluorescence emission of sulfur and nitrogen co‐doped carbon dots (S,N‐CDs) due to inner filter effect (IFE). The quenched fluorescence intensity of S,N‐CDs restored in the presence of isoniazid due to destroying CoOOH nanosheets by this drug. Moreover, with adding isoniazid the solution color of CoOOH nanosheets altered from brownish yellow to pale yellow. We exploited these facts to design a turn off–on fluorometric and paper‐based colorimetric sensor for isoniazid measurement at the range 0.5–5 and 5–100 μM with detection limits of 0.28 μM and 4.0 μM, respectively. The introduced dual sensor was used for pharmaceutical, environmental and biological analysis of isoniazid with satisfactory results. The paper‐based colorimetric sensor can be applied for isoniazid portable monitoring using a smartphone as a detector or even the naked eye.

show abstract

Colorimetric point-of-care paper-based sensors for urinary creatinine with smartphone readout

Cited by 69 publications

References 25 publications

Smartphone-Assisted Protein to Creatinine Ratio Determination on a Single Paper-Based Analytical Device

Smartphone-Assisted Protein to Creatinine Ratio Determination on a Single Paper-Based Analytical Device

A Point‐of‐Care Sensing Platform for Multiplexed Detection of Chronic Kidney Disease Biomarkers Using Molecularly Imprinted Polymers

A turn off–on fluorometric and paper‐based colorimetric dual‐mode sensor for isoniazid detection

Contact Info

Product

Resources

About