Colorimetric determination of N-arylhydroxylamines with 9-chloroacridine

Abstract

sis. SRM paint was analyzed by polarography and atomic absorption. The reasonably good agreement between 252Cf activation analysis and the other methods demonstrates the reliability and utility of the isotope source technique for this determination.Irradiation for 2 hours with the 600-Mg 252Cf source followed by 100 minutes of counting with a 60-cm3 Ge(Li) detector yields about 7 counts of the 204>«Pb isotope per milligram of lead. For a paint sample weighing 1.5 grams, the lower limit of determination would be of the order of 1%.As noted, analysis of the Ge(Li) spectrum of those elements likely to be major or minor constituents of paint indicates that they will not interfere in the quantitation of the gamma ray lines of 204mPb. Self-shielding effects due to the matrix are expected to be negligible since the nuclear reaction involves fast neutrons and a paint sample is unlikely to contain appreciable amounts of neutron moderating elements.The results presented here indicate that with a 252Cf source of several tens of milligrams and the 60-cm3 Ge(Li) detector, the determination of •~1% of lead in a sample of 1-2 grams of paint could be accomplished with a 15-minute irradiation and 15-minute counting. The potential exists for largely automating the irradiation and counting of a large number of samples. Hence, it would appear possible that although the initial cost of equipment for this determination would be greater than for alternate methods, the speed and adequate reliability of the isotope source method would make it economically competitive.

Colorimetric Determination of Paracetamol Using 9-Chloroacridine Reagent: Application to Pharmaceutical Formulations

“…The colour produced from the reaction of hydrolyzed paracetamol with 9-CA suggested that a free amino function in the molecule is necessary for the reaction. This finding is in agreement with the reaction of primary aromatic amines and/or aromatic hydroxylamine's with the acridine (Gammans et al, 1974;Stewart and Lotti, 1970;Shaw et al, 1969;Ray et al, 1969) to form highly coloured solutions. However, the reaction mechanism has been explained in Scheme (1).…”