Colorimetric Determination of Isoniazid with 9-Chloroacridine

Stewart, James T.; Settle, David A.

doi:10.1002/jps.2600640834

Cited by 21 publications

(4 citation statements)

References 11 publications

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“…10 (2021) has an excellent precision. The interaction of TCs with 9-CA to give a highly colored substituted 9-chloroacridine has been noticed [27]. The unstable 9-chloro derivative was modified to stable 9-phenoxy derivative by reacting with an excess phenol.…”

Section: Introductionmentioning

confidence: 99%

Using 9-Chloroacridine as A Chromogenic Reagent for The Detection of Tetracycline

2021

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An accurate, cost-effective and simple method has been described for the quantitative determination of Tetracycline hydrochloride (TCs) in bulk and pharmaceutical samples using an aqueous medium. The proposed method is based on the nucleophilic substitution reaction of 9-chloroacridine (9-CA) as a chromogenic reagent with TCs in an aqueous solution forming a colored complex. The spectrum of the product shows maximum absorption at 385 nm against the blank. The linearity range of the calibration graph was (1-28) μg.mL -1 with a limited detection value (LOD) 0.138 μg.mL -1 . The results show the absence of interferences from the excipients compounds on the determination of the drug. The suggested method exhibited high reproducibility (RSD) 2.3 % with mean percentage recovery (R%) 99.95. Furthermore, the stability constant has been calculated to be 9.843×10 5 L.mol -1 and the reaction mechanism is proposed. A ratio of 1: 1 was found between the above drug and the reagent using Job and molar ratio methods. The accuracy and validity of the proposed method were further determined by performing a recovery experiment by the use of the standard addition method. It is found that the method does not require extraction process and it agree well standard addition method. Application of the suggested method to commercial pharmaceutical preparations (capsule and ointment) is presented.

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Section: Introductionmentioning

confidence: 99%

Using 9-Chloroacridine as A Chromogenic Reagent for The Detection of Tetracycline

2021

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“…Visible spectrophotometry is by far the most widely used technique for the assay of INH. Methods were based on a variety of reaction schemes such as nucleophilic substitution [45], condensation [46][47][48][49], charge-transfer and ionpair [50], derivatization [51][52][53][54], diazo-coupling [55,56], oxidative coupling [57,58], complex formation [59][60][61], and redox followed by complexation [62][63][64][65]. These methods suffer from one or more of the disadvantages such as drastic experimental conditions, use of organic solvent, longer standing time, poor sensitivity, and narrow linear range.…”

Section: Introductionmentioning

confidence: 99%

Redox-Reaction Based Spectrophotometric Assay of Isoniazid in Pharmaceuticals

Swamy

Prashanth

Basavaiah

2014

ISRN Analytical Chemistry

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Two spectrophotometric methods are described for the determination of isoniazid (INH) in pharmaceuticals. In the first method (FCR method), INH is reacted with Folin-Ciocalteu reagent in Na2CO3 medium and the resulting blue colored chromogen measured at 760 nm. Iron(II), formed as a result of reaction between INH and iron(III), is made to react with ferricyanide, and the resulting Prussian blue is measured at 760 nm, basing the second method (FFC method). The conditions for better performance are optimized. Beer’s law is obeyed in the concentration ranges 0.5–10 and 0.2–3.0 μg mL−1 for FCR method and FFC methods, respectively, with corresponding molar absorptivity values of 1.12×104 and 4.55×104 L mol−1 cm−1. The methods are validated for accuracy, precision, LOD, LOQ, robustness, and ruggedness as per the current ICH guidelines. The validated methods were successfully applied to quantify INH in its commercial formulation with satisfactory results; hence the methods are suitable for isoniazid determination in bulk drugs and pharmaceuticals.

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“…The repellency factors from Skinner et al (10) are: RFcu, -2; RFcii, 52; and RFCISU, 17. The repellency factors from Skinner et al (10) are: RFcu, -2; RFcii, 52; and RFCISU, 17.…”

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confidence: 99%