Colorimetric determination of N-acetylhexosamine-terminating O-glycosidically linked saccharides in mucins and glycoproteins

Bhavanandan, V. P.; Sheykhnazari, Mostafa; Devaraj, Halagowder

doi:10.1016/0003-2697(90)90542-h

Cited by 27 publications

(9 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The cumulative weight of secreted saliva was measured every 15 s. Fluid secretion was calculated from time-differentiation of the weight. N-Acetylgalactosamine in the saliva was measured colorimetrically and used as an indicator of mucin secretion (Bhavanandan et al, 1990).…”

Section: Methodsmentioning

confidence: 99%

Dose-Dependent Morphological Changes of Intercellular Canaliculi during Stimulation with Carbachol and Isoproterenol in the Isolated Rat Submandibular Gland

Murakami

Saito²,

Puxeddu³

et al. 2003

European Journal of Morphology

View full text Add to dashboard Cite

Intercellular canaliculi (IC) form a primary mixing reservoir for transcellularly and paracellularly secreted saliva whose composition depends on the degree of elevation of cytosolic Ca2+ and of cytosolic cyclic AMP concentrations caused by the secretagogues employed. In perfused rat submandibular gland (SMG), appearance of exocytosis on IC reflected the quantity of secreted mucin. Morphological observations were carried out by HR-SEM using a modified osmium maceration method on specimens treated with CCh and/or ISP. Mild secretory stimulation revealed that exocytosis did not occur simultaneously, even along the same intercellular canaliculus. Higher doses did not alter the spatial distribution of exocytosis along intercellular canaliculi but increased its temporal frequency, dose dependently. These findings lead us to conclude that, under low levels of secretory stimulation, exocytosis does not show a dose-dependent change, but that its spatial and temporal frequency changes in a dose-dependent manner.

show abstract

Section: Methodsmentioning

confidence: 99%

Dose-Dependent Morphological Changes of Intercellular Canaliculi during Stimulation with Carbachol and Isoproterenol in the Isolated Rat Submandibular Gland

Murakami

Saito²,

Puxeddu³

et al. 2003

European Journal of Morphology

View full text Add to dashboard Cite

show abstract

“…On the subsequent day, the activity of the enzyme was analysed by Morgan-Elson assay [21] by utilising mucin as a substrate.…”

Section: Plasmid Dna Isolationmentioning

confidence: 99%

“…The enzyme was also isolated from the wild culture (Cr)). The activity of endo-a-N-acetyl galactosaminidase isolated from (Cr+) and (Cr)) cultures was compared by monitoring the release of terminal dissacharide from mucin (10 mg/ml) by Morgan-Elson assay [21].…”

Section: Plasmid Dna Isolationmentioning

confidence: 99%

“…The dialysate was applied to Sephacryl S-200 column and 50, 3 ml fractions were collected. The protein content in the fraction was determined by LowryÕs method [20] and the enzyme assay was carried out by the Morgan-Elson method [21]. The fractions showing positive enzyme activity were pooled, dialysed against deionised water and stored after lyophilisation.…”

Section: Plasmid Dna Isolationmentioning

confidence: 99%

See 1 more Smart Citation

A Schiff base complex of chromium(III): an efficient inhibitor for the pathogenic and invasive potential of Shigella dysenteriae

Shrivastava

Devaraj

Nair

2004

Journal of Inorganic Biochemistry

View full text Add to dashboard Cite

“…A sensitive colorimetric assay for detecting mucin was described by Bhavanandan et al [28]. Ten microliters of 0.13 M sodium borate buffer, pH 10 was added to 30 µl of cell lysates or mucin standard in 96-well plate and mixed by shaking the plate for 5 min.…”

Section: Determination Of Mucin Level Of Colon Epithelial Goblet Cellsmentioning

confidence: 99%

In Vitro Sustained Differentiation of Rat Colon Epithelial Stem Cells

Abu‐Serie¹,

Demellawy²,

El‐Sayed³

et al. 2016

Biochem Anal Biochem

View full text Add to dashboard Cite

Colonic epithelium composes of various cell types including alkaline phosphate-expressing absorptive, mucussecreting goblet and neuroendocrine cells that are derived from stem cells through asymmetric division. The continuous renewal of stem cells occurs under the highly coordinated cellular redox state. In the current study, based on a comparison with other culture media, colon epithelial cells were able to be sustained in vitro with normal status for more than two months under the chosen culture condition; α-MEM medium containing 20% fetal bovine serum. The cultured epithelial cells had normal doubling time and normal morphological characteristics as examined by transmission electron microscope. Also, these cultured cells contained functional stem cells and maintained their differentiation potency of colon stem cells, compared with freshly isolated mucosal epithelial cells, as indicated by the maintaining of aldehyde dehydrogenase 1B1 expression (11.31 ± 0.45 to 11.15 ± 0.48), ability to reduce silver nitrate, alkaline phosphate activity (0.513 ± 0.007 mU/μg to 0.438 ± 0.005 mU/μg), mucin secretion (34.71 ± 0.714 μg/ml to 32.93 ± 0.357 μg/ml) in appropriate cellular redox state level (-258.4 ± 1.3 mV to -237.4 ± 3.7 mV). The present study showed sustaining replication potential and functional differentiation of colonic epithelial stem cell population in this culture. The above culture system may be useful as an in vitro model for stemness, toxicological, and carcinogenesis studies.

show abstract

Colorimetric determination of N-acetylhexosamine-terminating O-glycosidically linked saccharides in mucins and glycoproteins

Cited by 27 publications

References 23 publications

Dose-Dependent Morphological Changes of Intercellular Canaliculi during Stimulation with Carbachol and Isoproterenol in the Isolated Rat Submandibular Gland

Dose-Dependent Morphological Changes of Intercellular Canaliculi during Stimulation with Carbachol and Isoproterenol in the Isolated Rat Submandibular Gland

A Schiff base complex of chromium(III): an efficient inhibitor for the pathogenic and invasive potential of Shigella dysenteriae

In Vitro Sustained Differentiation of Rat Colon Epithelial Stem Cells

Contact Info

Product

Resources

About