Colorimetric detection of pathogenic bacteria using platinum-coated magnetic nanoparticle clusters and magnetophoretic chromatography

Kwon, Donghoon; Lee, Sang‐Hee; Ahn, Myung Mo; Kang, In Seok; Park, Ki-Hwan; Jeon, Sangmin

doi:10.1016/j.aca.2015.04.044

Cited by 45 publications

(32 citation statements)

References 28 publications

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“…Compared to the free SAMNPs, the cell-SAMNPs are separated more efficiently since the magnetic force exerted on the magnetic particles is proportional to their apparent volume [9]. The small size of SAMNPs helps to retain the superparamagnetic properties [14].…”

Section: Resultsmentioning

confidence: 99%

“…This issue was addressed by adapting magnetophoretic chromatography using a liquid-type filter [8, 9]. Before magnetophoretic separation, all of the magnetic objects were dispersed uniformly in the upper layer of liquid in the pipette tip.…”

Section: Resultsmentioning

confidence: 99%

“…This problem can be addressed by adapting magnetophoretic chromatography using a liquid-type filter [8, 9]. A solution containing the free SAMNPs, free RNA-SAMNPs complex and chromatin-SAMNPs complex are loaded onto the surface of a viscous PEG solution in a 200-μl pipette tip to form two separated liquid layers due to differences in viscosities.…”

Section: Introductionmentioning

confidence: 99%

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Rapid and unbiased extraction of chromatin associated RNAs from purified native chromatin

Zhou

Yang

Konieczny

et al. 2015

Journal of Chromatography A

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An ultra fast and unbiased method that uses salicylic acid coated magnetic nanoparticles (SAMNPs) and magnetophoretic chromatography is developed to extract chromatin associated RNAs (CARs). The SAMNPs were first used for enriching cells from the cell culture media and further used for capturing chromatin after cells were lysed. The formed SAMNPs-chromatin complexes were transferred to a viscous polyethylene glycol (PEG) solution stored in a 200-μL pipette tip. Due to the difference in viscosities, a bi-layer liquid was formed inside the pipette tip. The SAMNPs-chromatin complexes were separated from the free SAMNPs and free RNA-SAMNPs complexes by applying an external magnetic field. The CARs were further extracted from the SAMNP-chromatin complexes directly. The extracted CARs were reverse transcribed as cDNA and further characterized by real-time qPCR. The total assay time taken for cell separation, chromatin purification and chromatin associated RNAs extraction can be accomplished in less than 2h.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Rapid and unbiased extraction of chromatin associated RNAs from purified native chromatin

Zhou

Yang

Konieczny

et al. 2015

Journal of Chromatography A

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“…Metals such as gold, silver, zinc, and platinum show good prospects to enhance magnetization and optical and electrochemical properties of materials. [42][43][44][45][46] Silva et al and Elsherbini et al reported the synthesis and biomedical applications, such as drug delivery, hyperthermia, photothermal therapy, and imaging, of gold-coated iron oxide nanoparticles. 42,43 Silver is an excellent antibacterial agent.…”

mentioning

confidence: 99%

“…44 Platinum coating over magnetic nanoparticle is very less reported, but Kwon et al synthesized platinum-coated magnetic nanoparticles for colorimetric detection of pathogenic bacteria and magnetophoretic chromatography application. 45 Thomas et al developed zinc-doped iron oxide nanocrystals with mesoporous silica framework as drug delivery vehicle for controlled release of drug molecules achieved by external magnetic hyperthermia effect. 46 For magnetic nanoparticles to be efficiently used in medicine, they should be extremely biocompatible, stable, and securely eliminated from the body system.…”

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confidence: 99%

Magnetic hydroxyapatite: a promising multifunctional platform for nanomedicine application

Mondal¹,

Manivasagan²,

Bharathiraja³

et al. 2017

IJN

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In this review, specific attention is paid to the development of nanostructured magnetic hydroxyapatite (MHAp) and its potential application in controlled drug/gene delivery, tissue engineering, magnetic hyperthermia treatment, and the development of contrast agents for magnetic resonance imaging. Both magnetite and hydroxyapatite materials have excellent prospects in nanomedicine with multifunctional therapeutic approaches. To date, many research articles have focused on biomedical applications of nanomaterials because of which it is very difficult to focus on any particular type of nanomaterial. This study is possibly the first effort to emphasize on the comprehensive assessment of MHAp nanostructures for biomedical applications supported with very recent experimental studies. From basic concepts to the real-life applications, the relevant characteristics of magnetic biomaterials are patented which are briefly discussed. The potential therapeutic and diagnostic ability of MHAp-nanostructured materials make them an ideal platform for future nanomedicine. We hope that this advanced review will provide a better understanding of MHAp and its important features to utilize it as a promising material for multifunctional biomedical applications.

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Antibody‐free bioimprint aided sandwich ELISA technique for cell recognition and rapid screening for bacteria

2020

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We have developed and tested a novel ELISA-like approach for bacterial detection based upon selective adhesion of targeted bacteria to microwells with prefabricated bacterial bioimprints. Bioimprints were produced from three bacterial species; Escherichia coli (Gram-negative), Rhodococcus rhodochrous (Gram-positive) and Sarcina aurantiaca (Gram-negative), by using molding with curable silicone from dense layers of bacterial cells deposited on a glass substrate. We demonstrated that the surface functionalized whole cell bioimprints were able to selectively recognize and bind their own bacterial cell type. In order to detect target bacteria that are bound to the matching bioimprint, we also developed silica nanoparticles dual-functionalized with (3-glycidyloxypropyl)trimethoxysilane (GLYMO) coupled with 4-hydroxyphenylboronic acid (4-HPBA), SiO 2 NPs/GLYMO/4-HPBA, which were further conjugated with horseradish peroxidase (HRP). Bacterial detection was demonstrated to work in the established ELISA-like protocol using the colorimetric reaction of the conjugated HRP with 3,3′,5,5′tetramethylbenzidine (TMB). The bioimprints were used instead of capture antibodies and HRP-coated dual functionalized silica nanoparticles instead of a secondary antibody with TMB as the enzyme-converted reagent, producing a colored byproduct. This bacterial bioimprint-based detection method does not rely on any antibodies, uses stable and inexpensive reagents, and could potentially find application for rapid diagnostics of bacterial pathogens in clinical samples at the point of care. K E Y W O R D S bacteria, bioimprints, ELISA, functionalized silica nanoparticles, horse radish peroxidase 1 INTRODUCTION Bacterial infections remain as a serious issue in modern health care. [1-3] In recent years, this need for the rapid diagnosis of bacterial infections has resulted in the This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Colorimetric detection of pathogenic bacteria using platinum-coated magnetic nanoparticle clusters and magnetophoretic chromatography

Cited by 45 publications

References 28 publications

Rapid and unbiased extraction of chromatin associated RNAs from purified native chromatin

Rapid and unbiased extraction of chromatin associated RNAs from purified native chromatin

Magnetic hydroxyapatite: a promising multifunctional platform for nanomedicine application

Antibody‐free bioimprint aided sandwich ELISA technique for cell recognition and rapid screening for bacteria

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