Colorimetric detection of influenza A (H1N1) virus by a peptide-functionalized polydiacetylene (PEP-PDA) nanosensor

Song, Sinae; Ha, Kab; Guk, Kyeonghye; Hwang, Seul-Gee; Choi, Jong Min; Kang, Taejoon; Bae, Pan‐Kee; Jung, Juyeon; Lim, Eun‐Kyung

doi:10.1039/c6ra06689e

Cited by 52 publications

(45 citation statements)

References 55 publications

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“…The sensitivity of the bioassay is 0.53 copy per lL, which is comparable to RT-PCR. Furthermore, the H1N1 virus can be detected, using PDA functionalized by peptide (PEP-PDA) colorimetric nanosensor, where the PDA is formed by nanoprecipitation [159]. At the same time, PEP is irradiated with 256 nm UV light, which causes the PEP to have special recognition to the H1N1 virus.…”

Section: Polymerized Polydiacetylenementioning

confidence: 99%

“…At the same time, PEP is irradiated with 256 nm UV light, which causes the PEP to have special recognition to the H1N1 virus. This PEP is then attached onto the surface of the PDA vesicles in the presence of EDC and NHS linkers [159]. In the presence of H1N1 virus, the color of the PDA changes from blue to red due to the specific binding of H1N1 to PEP.…”

Section: Polymerized Polydiacetylenementioning

confidence: 99%

“…In the presence of H1N1 virus, the color of the PDA changes from blue to red due to the specific binding of H1N1 to PEP. This color change is due to higher stress, which causes a strain in the conjugated backbone of PDA vesicles to increase energy gap, then allowing for the absorption of higher energy photons, thus causing the color of the PDA vesicle solution to change from blue to red [159].…”

Section: Polymerized Polydiacetylenementioning

confidence: 99%

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Colorimetric biosensors for point-of-care virus detections

Zhao

Wong

Zheng

et al. 2020

Materials Science for Energy Technologies

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a b s t r a c tColorimetric biosensors can be used to detect a particular analyte through color changes easily by naked eyes or simple portable optical detectors for quantitative measurement. Thus, it is highly attractive for point-of-care detections of harmful viruses to prevent potential pandemic outbreak, as antiviral medication must be administered in a timely fashion. This review paper summaries existing and emerging techniques that can be employed to detect viruses through colorimetric assay design with detailed discussion of their sensing principles, performances as well as pros and cons, with an aim to provide guideline on the selection of suitable colorimetric biosensors for detecting different species of viruses. Among the colorimetric methods for virus detections, loop-mediated isothermal amplification (LAMP) method is more favourable for its faster detection, high efficiency, cheaper cost, and more reliable with high reproducible assay results. Nanoparticle-based colorimetric biosensors, on the other hand, are most suitable to be fabricated into lateral flow or lab-on-a-chip devices, and can be coupled with LAMP or portable PCR systems for highly sensitive on-site detection of viruses, which is very critical for early diagnosis of virus infections and to prevent outbreak in a swift and controlled manner.

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Section: Polymerized Polydiacetylenementioning

confidence: 99%

Section: Polymerized Polydiacetylenementioning

confidence: 99%

Section: Polymerized Polydiacetylenementioning

confidence: 99%

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Colorimetric biosensors for point-of-care virus detections

Zhao

Wong

Zheng

et al. 2020

Materials Science for Energy Technologies

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“…[ 56 , 57 , 58 ]: sialic acid was grafted on β-glucoside and sialic and lactose moieties were grafted on a glucoside chain for insertion in the PDA layer. Different other types of virus receptor were grafted onto PDA moieties: antibodies [ 59 , 60 ] and peptides [ 61 ].…”

Section: Pda Vesicle Based Assays For Detection Of Influenza Virusmentioning

confidence: 99%

Responsive Polydiacetylene Vesicles for Biosensing Microorganisms

Lebègue

Farre

Jose

et al. 2018

Sensors

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Polydiacetylene (PDA) inserted in films or in vesicles has received increasing attention due to its property to undergo a blue-to-red colorimetric transition along with a change from non-fluorescent to fluorescent upon application of various stimuli. In this review paper, the principle for the detection of various microorganisms (bacteria, directly detected or detected through the emitted toxins or through their DNA, and viruses) and of antibacterial and antiviral peptides based on these responsive PDA vesicles are detailed. The analytical performances obtained, when vesicles are in suspension or immobilized, are given and compared to those of the responsive vesicles mainly based on the vesicle encapsulation method. Many future challenges are then discussed.

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“…glucoside and sialic and lactose moieties were grafted on a glucoside chain for insertion in the PDA layer. Different other types of virus receptor were grafted onto PDA moieties: antibodies [58,59] and peptides [60]. The analytical performance of the different approaches is very difficult to evaluate, because of the use of different units.…”

Section: The Same Strategy Was Also Proposed In Ref 55 and 56 And In mentioning

confidence: 99%

Responsive Polydiacetylene Vesicles for Biosensing Microorganisms<strong> </strong>

Lebègue¹,

Farre²,

Jose³

et al. 2018

Preprint

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Colorimetric detection of influenza A (H1N1) virus by a peptide-functionalized polydiacetylene (PEP-PDA) nanosensor

Abstract: We developed a peptide-functionalized polydiacetylene nanosensor for pH1N1 virus detection with the naked eye.

Cited by 52 publications

References 55 publications

Colorimetric biosensors for point-of-care virus detections

Colorimetric biosensors for point-of-care virus detections

Responsive Polydiacetylene Vesicles for Biosensing Microorganisms

Responsive Polydiacetylene Vesicles for Biosensing Microorganisms<strong> </strong>

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Colorimetric detection of influenza A (H1N1) virus by a peptide-functionalized polydiacetylene (PEP-PDA) nanosensor

Abstract: ​We developed a peptide-functionalized polydiacetylene nanosensor for pH1N1 virus detection with the naked eye.

Cited by 52 publications

References 55 publications

Colorimetric biosensors for point-of-care virus detections

Colorimetric biosensors for point-of-care virus detections

Responsive Polydiacetylene Vesicles for Biosensing Microorganisms

Responsive Polydiacetylene Vesicles for Biosensing Microorganisms<strong> </strong>

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Resources

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Abstract: We developed a peptide-functionalized polydiacetylene nanosensor for pH1N1 virus detection with the naked eye.