Colorimetric detection of exosomal microRNA through switching the visible-light-induced oxidase mimic activity of acridone derivate

Xia, Yaokun; Chen, Tingting; Zhang, Li; Shi, Wanhua; Chen, Guan-Yu; Chen, Wenqian; Lan, Jianming; Li, Chunyan; Sun, Wei‐Ming; Chen, Jinghua

doi:10.1016/j.bios.2020.112834

Cited by 47 publications

(23 citation statements)

References 73 publications

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“…Finally, the convenient and controllable detection of exosomal miRNA remains challenging, because the adequately simple and robust assay platforms are lacking. A recent wok by Xia et al (2021) has developed a colorimetric strategy to detect exosomal miR-21 by switching the visible-light-induced oxidase mimic activity of acridone derivate. This may provide a feasible tool for the application in exosomal miRNAs-based diagnosis of PE.…”

Section: Discussionmentioning

confidence: 99%

Placenta-Derived MicroRNAs in the Pathophysiology of Human Pregnancy

et al. 2021

Front. Cell Dev. Biol.

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In placental mammals, reproductive success, and maternal-fetal health substantially depend on a well-being placenta, the interface between the fetus and the mother. Disorders in placental cells are tightly associated with adverse pregnancy outcomes including preeclampsia (PE), fetal growth restriction, etc. MicroRNAs (miRNAs) represent small non-coding RNAs that regulate post-transcriptional gene expression and are integral to a wide range of healthy or diseased cellular proceedings. Numerous miRNAs have been detected in human placenta and increasing evidence is revealing their important roles in regulating placental cell behaviors. Recent studies indicate that placenta-derived miRNAs can be released to the maternal circulation via encapsulating into the exosomes, and they potentially target various maternal cells to provide a hormone-like means of intercellular communication between the mother and the fetus. These placental exosome miRNAs are attracting more and more attention due to their differential expression in pregnant complications, which may provide novel biomarkers for prediction of the diseases. In this review, we briefly summarize the current knowledge and the perspectives of the placenta-derived miRNAs, especially the exosomal transfer of placental miRNAs and their pathophysiological relevance to PE. The possible exosomal-miRNA-targeted strategies for diagnosis, prognosis or therapy of PE are highlighted.

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Section: Discussionmentioning

confidence: 99%

Placenta-Derived MicroRNAs in the Pathophysiology of Human Pregnancy

et al. 2021

Front. Cell Dev. Biol.

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“…Up to date, some artificial peroxidase-like small molecules have been discovered and applied in colorimetric assays, including fluorescein and 10-methyl-2-aminoacridone [103,104]. Huang et al for the first time found that ethylene diaminetetraacetic acid (EDTA) exhibits an interesting photocatalytic property under light irradiation, which can catalyze the decomposition of hydrogen peroxide (H 2 O 2 ) into oxidative OH radical [105].…”

Section: Colorimetric Cytosensorsmentioning

confidence: 99%

Recent Development of Nanomaterials-Based Cytosensors for the Detection of Circulating Tumor Cells

2021

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The accurate analysis of circulating tumor cells (CTCs) holds great promise in early diagnosis and prognosis of cancers. However, the extremely low abundance of CTCs in peripheral blood samples limits the practical utility of the traditional methods for CTCs detection. Thus, novel and powerful strategies have been proposed for sensitive detection of CTCs. In particular, nanomaterials with exceptional physical and chemical properties have been used to fabricate cytosensors for amplifying the signal and enhancing the sensitivity. In this review, we summarize the recent development of nanomaterials-based optical and electrochemical analytical techniques for CTCs detection, including fluorescence, colorimetry, surface-enhanced Raman scattering, chemiluminescence, electrochemistry, electrochemiluminescence, photoelectrochemistry and so on.

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“…Researchers have developed various methods for miRNA detection, including microarray, Northern blotting, and RT-PCR. − Although the above traditional methods have high sensitivity, complicated operation and expensive equipment limit their development. Recently, a series of detection platforms have been explored, such as nucleic acid amplification strategy combined with surface-enhanced Raman spectroscopy (SERS), electrochemistry, − colorimetry, and fluorescence, which can significantly improve the analytical performance. Especially, the photoelectrochemical (PEC) detector adopts the mode of different types of energy and total separation of light (excitation signal) and photocurrent (detection signal).…”

Section: Introductionmentioning

confidence: 99%

“…13−15 Although the above traditional methods have high sensitivity, complicated operation and expensive equipment limit their development. Recently, a series of detection platforms have been explored, such as nucleic acid amplification strategy combined with surface-enhanced Raman spectroscopy (SERS), 16 electrochemistry, 17−19 colorimetry, 20 and fluorescence, 21 photocurrent (detection signal). Although PEC biosensor has various advantages including low background noise, low cost, and ultrahigh sensitivity and specificity, 22 the PEC detection platform was easily interfered with external environment factors.…”

Section: Introductionmentioning

confidence: 99%

“Dual-Signal-On” Integrated-Type Biosensor for Portable Detection of miRNA: Cas12a-Induced Photoelectrochemistry and Fluorescence Strategy

et al. 2021

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The abnormal expression of microRNA (miRNA) can affect the RNA transcription and protein translation, leading to tumor progression and metastasis. Currently, the accurate detection of aberrant expression of miRNA, particularly using a portable detection system, remains a great challenge. Herein, a novel dual-mode biosensor with high sensitivity and robustness for miR-21 detection was developed based on the cis-cleavage and trans-cleavage activities of Cas12a. miRNA can be combined with hairpin DNA-horseradish peroxidase anchored on a CdS/g-C 3 N 4 /B-TiO 2 photoelectrode, thus the nonenzymatic amplification was triggered to form numerous HRP-modified double-stranded DNA (HRP-dsDNA). Then, HRP-dsDNA can be specifically recognized and efficiently cis-cleaved by Cas12a nucleases to detach HRP from the substrate, while the remaining HRP on HRP-dsDNA can catalyze 4-chloro-1-naphthol (4-CN) to form biocatalytic precipitation (BCP) on the surface of the photoelectrode, and thus the photocurrent can be changed. Meanwhile, the trans-cleavage ability of Cas12a was activated, and nonspecifically degrade the FQ-reporter and a significant fluorescence signal can be generated. Such two different kinds of signals with independent transmission paths can mutually support to improve the performance of the detection platform. Besides, a portable device was constructed for the point-of-care (POC) detection of miR-21. Moreover, the dual-mode detection platform can be easily expanded for the specific detection of other types of biomarkers by changing the sequence of hairpin DNA, thereby promoting the establishment of POC detection for early cancer diagnosis.

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Colorimetric detection of exosomal microRNA through switching the visible-light-induced oxidase mimic activity of acridone derivate

Cited by 47 publications

References 73 publications

Placenta-Derived MicroRNAs in the Pathophysiology of Human Pregnancy

Placenta-Derived MicroRNAs in the Pathophysiology of Human Pregnancy

Recent Development of Nanomaterials-Based Cytosensors for the Detection of Circulating Tumor Cells

“Dual-Signal-On” Integrated-Type Biosensor for Portable Detection of miRNA: Cas12a-Induced Photoelectrochemistry and Fluorescence Strategy

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