Colorimetric detection of bisphenol A based on unmodified aptamer and cationic polymer aggregated gold nanoparticles

Zhang, Dongwei; Yang, Jiayi; Ye, Jing; Xu, Lurong; Xu, Hanchu; Zhan, Shenshan; Xia, Bing; Wang, Lumei

doi:10.1016/j.ab.2016.01.011

Cited by 66 publications

(19 citation statements)

References 36 publications

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“…As shown in Fig. 2, P4 aptamer, as a singlestranded DNA, has a negative peak at 240-260 nm and a positive peak at 270-290 nm, which is in accordance with the previous record [20,31]. After adding P4, the value of the negative and positive peaks changed, but the location of the peaks did not shift.…”

Section: Characterization Of the Interaction Between P4 Aptamer And Asupporting

confidence: 87%

“…In order to optimize the NaCl concentration, AuNPs were treated with different volumes of 2 M NaCl solution (0, 10,15,20,25,30,35, and 40 μL) and MOPS buffer were added to attain a total volume of 500 μL. After incubating for 5 min, A520 and A650 were recorded, and the minimum NaCl concentration that can fully induce aggregation of AuNPs was chosen.…”

Section: Optimization Of Detection Conditionsmentioning

confidence: 99%

“…When AuNPs aggregate and become larger nanoparticles, the colloidal solution will turn to blue gradually [19]. So AuNPs have been applied in colorimetric aptasensors on account of the aggregating behavior to detect diversified substances [20,21]. Moreover, compared with the modified AuNPs used in the colorimetric methods, unmodified AuNPs are less complicated since some modification and separation steps can be omitted [20].…”

Section: Introductionmentioning

confidence: 99%

“…So AuNPs have been applied in colorimetric aptasensors on account of the aggregating behavior to detect diversified substances [20,21]. Moreover, compared with the modified AuNPs used in the colorimetric methods, unmodified AuNPs are less complicated since some modification and separation steps can be omitted [20]. Thus, several metal ions and small organic molecules have been detected through the unmodified AuNP-based colorimetric assays [22].…”

Section: Introductionmentioning

confidence: 99%

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A label-free colorimetric progesterone aptasensor based on the aggregation of gold nanoparticles

Zhang

Xia

et al. 2016

Microchim Acta

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The authors describe an aptasensor for the detection of the gonadal hormone progesterone (P4) in aqueous solution. Gold nanoparticles (AuNPs) were coated with a P4-specific aptamer, and such particles do not aggregate in presence of NaCl due to the presence of the aptamer coating. If, however, progesterone is added, it will bind to the aptamer and release it from the surface. The uncoated AuNPs, on addition of NaCl, will aggregate and a color change from red (520 nm) to blue (650 nm) can be visually detected or photometrically quantified. The ratio of the absorbances at 650 and 520 nm is linearly related to the P4 concentration in the range from 2.6 to 800 nM. The complete detection range extends from 2.6 to 1400 nM, and the detection limit is 2.6 nM. Water containing various potential interferents, as well as tap water and urine, were spiked with P4 and the recoveries of P4 are in the range of 89.7-117.5%, 84.4-115.0% and 94.7-118.8%, respectively. This assay has a large potential with respect to the visual and instrumental determination of P4 in aquatic environment and urine.

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Section: Characterization Of the Interaction Between P4 Aptamer And Asupporting

confidence: 87%

Section: Optimization Of Detection Conditionsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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A label-free colorimetric progesterone aptasensor based on the aggregation of gold nanoparticles

Zhang

Xia

et al. 2016

Microchim Acta

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“…These disadvantages have prevented them from becoming widespread, making the capacity for on‐site detection rare. To facilitate the on‐site detection of BPA, aptamer‐based methods and enzyme‐linked immunosorbent assays (ELISAs) have been broadly investigated and developed . The use of aptamers and ELISA has allowed researchers to avoid the expense of purchasing high‐cost devices, making tremendous progress on the path toward on‐site detection.…”

Section: Introductionmentioning

confidence: 99%

Aminolated and Thiolated PEG‐Covered Gold Nanoparticles with High Stability and Antiaggregation for Lateral Flow Detection of Bisphenol A

Lin

Uzunoğlu

Stanciu

2017

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The few lateral flow assays (LFAs) established for detecting the endocrine disrupting chemical bisphenol A (BPA) have employed citrate-stabilized gold nanoparticles (GNPs), which have inevitable limitations and instability issues. To address these limitations, a more stable and more sensitive biosensor is developed by designing strategies for modifying the surfaces of GNPs with polyethylene glycol and then testing their effectiveness and sensitivity toward BPA in an LFA. Without the application of any enhancement strategy, this modified BPA LFA can achieve a naked-eye limit of detection (LOD) of 0.8 ng mL , which is 12.5 times better than the LOD of regular BPA LFAs, and a quantitative LOD of 0.472 ng mL . This modified LFA has the potential to be applied to the detection of various antigens.

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Fluorogenic Sensing of Carcinogenic Bisphenol A using Aptamer‐Capped Mesoporous Silica Nanoparticles

Ribes

Aznar

Bernardos

et al. 2017

Chemistry A European J

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Mesoporous silica nanoparticles loaded with rhodamine B and capped with a bisphenol A aptamer were used for the selective and sensitive detection of this lethal chemical. The pores of the nanoparticles are selectively opened in the presence of bisphenol A (through its selective coordination with the aptamer) with subsequent rhodamine B delivery. With this capped material a limit of detection as low as 3.5 μm of bisphenol A was measured.

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Colorimetric detection of bisphenol A based on unmodified aptamer and cationic polymer aggregated gold nanoparticles

Cited by 66 publications

References 36 publications

A label-free colorimetric progesterone aptasensor based on the aggregation of gold nanoparticles

A label-free colorimetric progesterone aptasensor based on the aggregation of gold nanoparticles

Aminolated and Thiolated PEG‐Covered Gold Nanoparticles with High Stability and Antiaggregation for Lateral Flow Detection of Bisphenol A

Fluorogenic Sensing of Carcinogenic Bisphenol A using Aptamer‐Capped Mesoporous Silica Nanoparticles

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