Colorimetric Detection by Gold Nanoparticle DNA Probes for Miltenberger Series (GP.Mur, GP.Hop, and GP.Bun) Identification

Abstract

AuNP DNA probes (RvB and RvA2) could be applied to distinguish the amplified products of Mi(+), Mi(-), and the blank by visual inspection and/or OD absorbance measurement.

Genotyping analysis of MNS blood group GP(B‐A‐B) hybrid glycophorins in the Chinese Southern Han population using a high‐resolution melting assay

“…Antisera to detect these antigens are scarce and are not commercially available presenting difficulty to screen for these hybrid glycophorins by serologic methods. With the introduction of molecular biology, DNA‐based methods such as polymerase chain reaction–restriction fragment length polymorphism (PCR‐RFLP) and PCR–sequence‐specific primer, which have been described and applied well previously, direct blood PCR, loop‐mediated isothermal amplification, gold nanoparticle DNA probes, and massive parallel sequencing, have been developed to genotype for specific low‐incidence antigens on hybrid glycophorins. Some of these methods either are laborious and time‐consuming, such as the need for gel electrophoresis, or can only be used for screening but are unable to identify the exact type of hybrid glycophorin.…”

Hybrid glycophorin and red blood cell antigen genotyping in Asian American type O blood donors with Mia phenotype

“…The real‐time PCR melting curve method has been used to identify the GYP*Mur allele from other Mi a ‐positive phenotypes . Colorimetric detection using gold nanoparticle oligonucleotide probes has been used in GP.Mur, GP.Hop, and GP.Bun identification . It is obvious that high‐throughput genotyping techniques will be the best choice of tools for screening Mi a ‐positive donors and identifying the variant alleles in the prevalent areas.…”