Colorimetric Chiral Recognition of Enantiomers Using the Nucleotide-Capped Silver Nanoparticles

Abstract

Chiral recognition is among the important and special modes of molecular recognition. It is highly desirable to develop a simple, rapid, sensitive, and high-throughput routine assay for chiral recognition. In this study, we demonstrate that nucleotide-capped Ag nanoparticles (AgNPs) can be used as an ultrahigh efficiency enantioseparation and detection platform for D- and L-cysteine. The aggregation of AgNPs is selectively induced by an enantiomer of cysteine, which allowed the rapid colorimetric enantiodiscrimination of cysteine without any prior derivatization and specific instruments and left an excess of the other enantiomer in the solution, thus resulting in enantioseparation. This is the first application of a nucleotide-capped AgNP-based biosensing platform for chiral recognition and opens new opportunities for design of more novel enantiosensing strategies and enantiospecific adsorbents and expansion of its application in different fields.

Fluorescent/luminescent detection of natural amino acids by organometallic systems

“…Therefore, enantioselectivity is one of the most important goals for the sensing of organic substances [190][191][192][193][194][195][196][197][198], because biological activity is strictly correlated with stereochemistry. Although some enantioselective methods based on capillary electrophoresis [199][200], voltammetry [201], colorimetric methods [202][203][204] etc. have been proposed, there are examples of enantioselective fluorescence sensors [205][206].…”

Colorimetric Nanoprobes

“…Ye et al developed an enantioseparation and detection platform for D-and L-cysteine using uridine 5'-triphosphate (UTP)-capped silver nanoparticles [96]. As seen in Fig.…”

Rapid and ultrasensitive colorimetric detection of mercury(II) by chemically initiated aggregation of gold nanoparticles

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