Colorimetric Chemosensor and Turn on Fluorescence Probe for pH Monitoring Based on Xanthene Dye Derivatives and its Bioimaging of Living Escherichia coli Bacteria

Mohamed, Mahmoud Basseem I.; Aysha, Tarek; Elmorsi, Taha M.; El‐Sedik, Mervat; Omara, Shimaa T.; Shaban, Elkhabiry; Kandil, Omaima M.; Bedair, A. H.

doi:10.1007/s10895-020-02522-1

Cited by 16 publications

(2 citation statements)

References 43 publications

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“…There are several classes of highly π‐conjugated chromophores suitable for studying pH changes with biological systems. The most common are xanthene (Mohamed et al, 2020), boron dipyrromethene (BODIPY) (Qiu et al, 2018) and pyrene (Hande et al, 2020) dyes. Xanthene dyes and their derivatives are useful for measuring the intracellular pH of living cells due to their varying intensities of fluorescence at different acidities (Martineau et al, 2017; Zhou et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

Comparison of two diphenyl polyenes as acid‐sensitive additives during the biodegradation of a thermoset polyester polyurethane coating

Shesham

Kelly

Burke

et al. 2021

J of Applied Microbiology

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Aims: Biochemical hydrolysis and chemical catalysis are involved in the successful biodegradation of polymers. In order to evaluate the potential separation between biochemical and chemical catalysis during the biodegradation process, we report the use of two diphenylpolyenes (DPPs), all trans-1,4-diphenylbutadiene (DPB) and all trans-1,6-diphenylhexatriene (DPH), as potential acid-sensitive indicators in polymers. Methods and Results: 1,4-Diphenylbutadiene and DPH (0.1% w/w) were melt-cast successfully with poly(ethylene succinate) hexamethylene (PES-HM) polyurethane (thermoset polyester polyurethane) coatings above 80℃. When these two DPP/ PES-HM coatings were exposed to a concentrated supernatant with significant esterase activity resulting from the growth of a recently isolated and identified strain of Tremellomycetes yeast (Naganishia albida 5307AI), the DPB coatings exhibited a measurable and reproducible localized decrease in the blue fluorescence emission in regions below where hydrolytic biodegradation was initiated in contrast with DPH blended coatings. The fluorescence changes observed in the biodegraded DPB coating were similar to exposing them to concentrated acids and not bases. Conclusions:Our experiments resulted in (1) a method to blend DPP additives into thermoset coatings, (2) the first report of the biodegradation of polyester polyurethane coating by N. albida, and (3) demonstration that hydrolytic supernatants from this strain generate acidic region within degrading polyester coatings using DPB as the indicator.Significance and Impact of the Study: Our experiments confirm that N. albida is an active polyester degrader and that DPB is a promising acid sensitive polymer coating additive.

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Section: Introductionmentioning

confidence: 99%

Comparison of two diphenyl polyenes as acid‐sensitive additives during the biodegradation of a thermoset polyester polyurethane coating

Shesham

Kelly

Burke

et al. 2021

J of Applied Microbiology

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“…Our findings could replace higher expensive pH instruments using handheld pH detection, and an intelligent smartphone system for everyone, even the chef in the kitchen, without the need for additional costly and timeconsuming experimental work.The pH value of different solutions is a particularly important point to determine the optimized conditions and quality control for industrial, biological, chemical, and environmental science either outdoor or indoor applications 1,2 . Hydrogen ions concentration [H + ] denoted to pH scales from 0 to 14, and the most methods counted for detection are complicated, expensive, and time-consuming as microelectrodes 3 , acid-based indicator 4 , potentiometric titration 1 , colorimetric and fluorescence probes application [5][6][7][8][9][10] . Currently, potentiometric measurements are the most technique used in pH detection.…”

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confidence: 99%

Facile and highly precise pH-value estimation using common pH paper based on machine learning techniques and supported mobile devices

Elsenety

Mohamed

Sultan

et al. 2022

Sci Rep

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Numerous scientific, health care, and industrial applications are showing increasing interest in developing optical pH sensors with low-cost, high precision that cover a wide pH range. Although serious efforts, the development of high accuracy and cost-effectiveness, remains challenging. In this perspective, we present the implementation of the machine learning technique on the common pH paper for precise pH-value estimation. Further, we develop a simple, flexible, and free precise mobile application based on a machine learning algorithm to predict the accurate pH value of a solution using an available commercial pH paper. The common light conditions were studied under different light intensities of 350, 200, and 20 Lux. The models were trained using 2689 experimental values without a special instrument control. The pH range of 1: 14 is covered by an interval of ~ 0.1 pH value. The results show a significant relationship between pH values and both the red color and green color, in contrast to the poor correlation by the blue color. The K Neighbors Regressor model improves linearity and shows a significant coefficient of determination of 0.995 combined with the lowest errors. The free, publicly accessible online and mobile application was developed and enables the highly precise estimation of the pH value as a function of the RGB color code of typical pH paper. Our findings could replace higher expensive pH instruments using handheld pH detection, and an intelligent smartphone system for everyone, even the chef in the kitchen, without the need for additional costly and time-consuming experimental work.

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Low-Temperature Processed Metal Oxides and Ion-Exchanging Surfaces as pH Sensor

Ugwuoke

Tagbo

Okwundu

et al. 2021

Chemically Deposited Nanocrystalline Metal Oxide Thin Films

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Colorimetric Chemosensor and Turn on Fluorescence Probe for pH Monitoring Based on Xanthene Dye Derivatives and its Bioimaging of Living Escherichia coli Bacteria

Cited by 16 publications

References 43 publications

Comparison of two diphenyl polyenes as acid‐sensitive additives during the biodegradation of a thermoset polyester polyurethane coating

Comparison of two diphenyl polyenes as acid‐sensitive additives during the biodegradation of a thermoset polyester polyurethane coating

Facile and highly precise pH-value estimation using common pH paper based on machine learning techniques and supported mobile devices

Low-Temperature Processed Metal Oxides and Ion-Exchanging Surfaces as pH Sensor

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