Colorectal cancer screening – Methodology

Jenkinson, Fiona; Steele, Robert

doi:10.1016/j.surge.2009.10.015

Cited by 31 publications

(23 citation statements)

References 44 publications

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“…However, identification of precancerous polyps, such as adenomatous polyps, is very challenging since the subtle macroscopic differentiation of subtype of polyps may not be apparent under a conventional white light reflectance (WLR) colonoscopy, which relies heavily on gross morphological changes of the epithelial tissue. 4 Thus, all polyps that are found during a colonoscopy are routinely resected and sent for histopathological examinations. However, the removal of polyps without malignancies could incur additional complications of perforation or bleeding, and an increase of cost and operational time during a colonoscopy.…”

Section: Introductionmentioning

confidence: 99%

In vivo diagnosis of colonic precancer and cancer using near-infrared autofluorescence spectroscopy and biochemical modeling

2011

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Section: Introductionmentioning

confidence: 99%

In vivo diagnosis of colonic precancer and cancer using near-infrared autofluorescence spectroscopy and biochemical modeling

2011

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“…CRC arises through a multi-step, multimechanism process consisting of initiation, promotion, and progression. Patients with CRC usually present with a change in bowel habits, rectal bleeding, abdominal pain, or weight loss [2]; however, most cases are diagnosed when the cancer is fairly advanced. Some genes and their expressed proteins, including APC, CTNNB1, KRAS2, BRAF, MADH4/SMAD4, TP53, PIK3CA, and TGFBR2, have been investigated with regards to the initiation and development of CRC [3][4].…”

mentioning

confidence: 99%

Cell-cycle arrest at G2/M and proliferation inhibition by adenovirus-expressed mitofusin-2 gene in human colorectal cancer cell lines

Cheng¹,

Zhou²,

Jiang³

et al. 2014

neo

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The mitofusin-2 (Mfn2) is a novel gene characterised as a cell proliferation inhibitor. Mfn2 protein over-expression, mediated by an adenovirus, has a significant anti-proliferative effect in hepatoma carcinoma cells. However, there is no report on the effect of Mfn2 on colorectal cancer (CRC). In this study, we found that Mfn2 protein and mRNA levels were downregulated in CRC tissues compared to nearby normal tissues. An adenovirus encoding the complete Mfn2 open reading frame (Ad-Mfn2) exhibited a prominent anti-proliferative effect in the CRC cell lines HCT 116, HT-29, and SW480. AdMfn2 infection significantly inhibited the proliferation of CRC cells compared with Ad-GFP infection. Mfn2 overexpression resulted in a cell-cycle arrest at G2/M phase in CRC cells, and it increased the levels of p-cdc2 (Tyr15) and Myt1; however, the levels of cyclin B1 and p-ERK1/2 was reduced. Mfn2 overexpression aslo increased the levels of active caspase-3 and cleaved PARP. Taken together, these findings indicate that Mfn2 has a significant anti-proliferative effect in CRC cells using adenoviral vectors.

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“…DNA is stable in faeces and, unlike blood, is shed continuously from malignant lesions. Other advantages over FOBT include lack of the requirement for pretest preparation and avoidance of 117 Chromosomal instability is a feature of most human tumours and is characterized by allelic imbalance due to loss or gain of distinct chromosomal regions. 118 It accounts for about 85% of sporadic colorectal cancer and almost all cancers in the familial syndrome adenomatous polyposis coli.…”

Section: Genetic Markersmentioning

confidence: 99%

New faecal tests in gastroenterology

Ayling¹

2011

Ann Clin Biochem

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Most abdominal disorders present with a limited number of overlapping symptoms. Blood tests are not routinely available for use in diagnosis and so investigation tends to require complex imaging procedures or endoscopy and biopsy. These are invasive for the patient, may be associated with morbidity and mortality and have considerable resource implications. Biochemical tests on a single sample of faeces are therefore a valuable alternative. Measurement of faecal calprotectin has been shown to have a role in the diagnosis of inflammatory bowel disease and in its monitoring. Lactoferrin is also of benefit used in this way. Faecal elastase has been demonstrated to be of use in the diagnosis of pancreatic insufficiency. A number of faecal markers have been explored in colorectal cancer. Faecal occult blood testing is used for population screening, but the metabolomic marker tumour, M2-pyruvate kinase, has potential for use in both diagnosis and screening. DNA testing has advantages in colorectal cancer but the exact applications of such tests require further evaluation.

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Colorectal cancer screening – Methodology

Cited by 31 publications

References 44 publications

In vivo diagnosis of colonic precancer and cancer using near-infrared autofluorescence spectroscopy and biochemical modeling

In vivo diagnosis of colonic precancer and cancer using near-infrared autofluorescence spectroscopy and biochemical modeling

Cell-cycle arrest at G2/M and proliferation inhibition by adenovirus-expressed mitofusin-2 gene in human colorectal cancer cell lines

New faecal tests in gastroenterology

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