In vivo diagnosis of colonic precancer and cancer using near-infrared autofluorescence spectroscopy and biochemical modeling

Shao, Xiaozhuo; Zheng, Wei; Huang, Zhiwei

doi:10.1117/1.3589099

Cited by 34 publications

(20 citation statements)

References 34 publications

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“…The increased hemoglobin content for colonic cancer could be attributed to the increased microvasculatures in malignant tumors [36,43]. The above observation is also consistent with previous NIR autofluorescence study in colonic cancer [44]. Further, the changes of tissue microstructural scattering properties (e.g.…”

Section: Resultssupporting

confidence: 80%

“…Interestingly, m 22 and m 33 element are identical not only for normal colon tissues, but also for the cancerous colon tissues. Besides, the magnitude of m 22 and m 33 are much higher than that of m 44 , indicating that the backscattered light is less depolarized when the incident light is linearly rather than circularly polarized. The magnitudes of m 22 and m 33 are higher for colon cancer than normal colon tissue, indicating a lower depolarization power of colon cancer tissues.…”

Section: Resultsmentioning

confidence: 98%

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Integrated Mueller-matrix near-infrared imaging and point-wise spectroscopy improves colonic cancer detection

Wang

Zheng

Lin

et al. 2016

Biomed. Opt. Express

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Abstract:We report the development and implementation of a unique integrated Mueller-matrix (MM) near-infrared (NIR) imaging and Muellermatrix point-wise diffuse reflectance (DR) spectroscopy technique for improving colonic cancer detection and diagnosis. Point-wise MM DR spectra can be acquired from any suspicious tissue areas indicated by MM imaging. A total of 30 paired colonic tissue specimens (normal vs. cancer) were measured using the integrated MM imaging and point-wise MM DR spectroscopy system. Polar decomposition algorithms are employed on the acquired images and spectra to derive three polarization metrics including depolarization, diattentuation and retardance for colonic tissue characterization. The decomposition results show that tissue depolarization and retardance are significantly decreased (p<0.001, paired 2-sided Student's t-test, n = 30); while the tissue diattentuation is significantly increased (p<0.001, paired 2-sided Student's t-test, n = 30) associated with colonic cancer. Further partial least squares discriminant analysis (PLS-DA) and leave-one tissue site-out, cross validation (LOSCV) show that the combination of the three polarization metrics provide the best diagnostic accuracy of 95.0% (sensitivity: 93.3%, and specificity: 96.7%) compared to either of the three polarization metrics (sensitivities of 93.3%, 83.3%, and 80.0%; and specificities of 90.0%, 96.7%, and 80.0%, respectively, for the depolarization, diattentuation and retardance metrics) for colonic cancer detection. This work suggests that the integrated MM NIR imaging and point-wise MM NIR diffuse reflectance spectroscopy has the potential to improve the early detection and diagnosis of malignant lesions in the colon. #258157Received 25

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Section: Resultssupporting

confidence: 80%

Section: Resultsmentioning

confidence: 98%

Integrated Mueller-matrix near-infrared imaging and point-wise spectroscopy improves colonic cancer detection

Wang

Zheng

Lin

et al. 2016

Biomed. Opt. Express

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“…One of them is due to the background noise caused by excitation leaks and fluorescence from superficial layers. While the natural fluorescence of tissues may be used as a means of study, [5][6][7][8] it is an obstacle for fluorescence reflectance imaging because it causes the depths addressed to be small (several millimeters): the detected signals decrease exponentially with depth while the background noise remains the same.…”

Section: Introductionmentioning

confidence: 99%

Laser line scanning illumination scheme for the enhancement of contrast and resolution for fluorescence reflectance imaging

Fantoni¹,

Hervé²,

Poher³

et al. 2013

SPIE Proceedings

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International audienceIntraoperative fluorescence imaging in reflectance geometry is an attractive imaging modality as it allows to noninvasively monitor fluorescence targeted tumors located below the tissue surface. The drawbacks of this technique are the poor resolution in the axial and lateral directions due to multiple light scattering and background fluorescence decreasing the contrast. We propose a novel fluorescence imaging method based on laser line illumination in reflectance geometry. We scan the medium with the laser line and acquire images at each position of the line. We then detect only single stripes of each image located on the excitation line or farther from it. We can also subtract the surrounding signal to the detected stripe, the optimal detection scheme depending on the depth of the object of interest. This allows us to reduce the contribution of parasite signals such as background fluorescence or excitation leaks and also enhances the resolution. These operations on the images can either be digitally done in post-processing or can directly be hardware implemented, allowing our method to be integrated in a handheld device for real-time use. This technique has been validated with tissue-like liquid phantoms with different levels of background fluorescence. Fluorescent inclusions are observed in several configurations at depths ranging from 1 mm to 1 cm. Our results are compared to those obtained with a more classical wide-field detection scheme. Finally, we propose a setup to optically implement the masking detection that will dramatically fasten the detection scheme and optimize the fluorescence light throughput of the system

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“…The architectural and biochemical differences between a normal and a cancerous tissue affect the interaction of light with the tissue and produce different spectral signatures in both cases, thus allowing the tissues to be differentiated. Autofluorescence [30][31][32][33] and reflectance [34][35][36][37] spectroscopy are techniques that extract these spectral signatures usually using blue light and white light, respectively. Both techniques are currently largely being actively investigated by different groups around the world.…”

Section: Introductionmentioning

confidence: 99%

Optical spectroscopy combined with high-resolution magnetic resonance imaging for digestive wall assessment: endoluminal bimodal probe conception and characterization in vitro, on organic sample and in vivo on a rabbit

et al. 2011

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Colorectal cancer is a major health issue worldwide. Conventional white light endoscopy (WLE) coupled to histology is considered as the gold standard today and is the most widespread technique used for colorectal cancer diagnosis. However, during the early stages, colorectal cancer is very often characterized by flat adenomas which develop just underneath the mucosal surface. The use of WLE, which is heavily based on the detection of morphological changes, becomes quite delicate due to subtle or quasi-invisible morphological changes of the colonic lining. Several techniques are currently being investigated in the scope of providing new tools that would allow such a diagnostic or assist actual techniques in so doing. We hereby present a novel technique where high spatial resolution MRI is combined with autofluorescence and reflectance spectroscopy in a bimodal endoluminal probe to extract morphological data and biochemical information, respectively. The design and conception of the endoluminal probe are detailed and the promising preliminary results obtained in vitro (home-built phantom containing eosin and rhodamine B), on an organic sample (the kiwi fruit) and in vivo on a rabbit are presented and discussed.

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In vivo diagnosis of colonic precancer and cancer using near-infrared autofluorescence spectroscopy and biochemical modeling

Cited by 34 publications

References 34 publications

Integrated Mueller-matrix near-infrared imaging and point-wise spectroscopy improves colonic cancer detection

Integrated Mueller-matrix near-infrared imaging and point-wise spectroscopy improves colonic cancer detection

Laser line scanning illumination scheme for the enhancement of contrast and resolution for fluorescence reflectance imaging

Optical spectroscopy combined with high-resolution magnetic resonance imaging for digestive wall assessment: endoluminal bimodal probe conception and characterization in vitro, on organic sample and in vivo on a rabbit

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