Colonization pattern of primary tomato roots by Pseudomonas fluorescens A6RI characterized by dilution plating, flow cytometry, fluorescence, confocal and scanning electron microscopy

Gamalero, Elisa; Lingua, Guido; Caprì, Flavia; Fusconi, Anna; Berta, Graziella; Lemanceau, Philippe

doi:10.1016/j.femsec.2003.12.012

Cited by 89 publications

(63 citation statements)

References 54 publications

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“…The abundance of B. subtilis B068150 in rhizosphere was significantly higher than in non-rhizosphere in the three soils (clay, p = 0.021; loam, p = 0.04; sand, p = 0.002). The colonization in the cucumber rhizosphere in the three soils may be related to root exudates as previously reported [53,54]. Our data suggest that the survival of strain B068150 in cucumber rhizosphere and non-rhizosphere was successful, indicating positive impacts on biocontrol of Fusarium wilt as previously reported [55].…”

Section: Discussionsupporting

confidence: 85%

Cucumber Rhizosphere Microbial Community Response to Biocontrol Agent Bacillus subtilis B068150

Ibekwe

et al. 2015

Agriculture

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Gram-positive bacteria Bacillus subtilis B068150 has been used as a biocontrol agent against the pathogen Fusarium oxysporum cucumerinum. Cucumber was grown in three soils with strain B068150 inoculated in a greenhouse for 90 days, and the colonization ability of strain B068150 in cucumber rhizosphere and non-rhizosphere soils was determined. Changes in total bacteria and fungi community composition and structures using denaturing gradient gel electrophoresis (DGGE) and sequencing were determined. Colony counts showed that B068150 colonization in the rhizosphere was significantly higher (p < 0.001) than in non-rhizosphere soils. Based on our data, the introduction of B. bacillus B068150 did not change the diversity of microbial communities significantly in the rhizosphere of three soils. Our data showed that population density of B068150 in clay soil had a significant negative correlation on bacterial diversity in cucumber rhizosphere in comparison to loam and sandy soils, suggesting that the impact of B068150 might be soil specific.

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Section: Discussionsupporting

confidence: 85%

Cucumber Rhizosphere Microbial Community Response to Biocontrol Agent Bacillus subtilis B068150

Ibekwe

et al. 2015

Agriculture

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“…Different population densities were reported for the diverse root zones. This has been well described by Gamalero et al (2004) with Pseudomonas fluorescens strain A6RI and tomato roots, where the distribution and density of the inoculant strain varied according to the root zone. Non-uniform bacterial colonization along the root can be explained by different factors such as varying root exudation patterns, bacterial quorum sensing effects as well as many others, which are summarized in Table 1 and described below in greater detail.…”

Section: Rhizosphere and Rhizoplane Colonizationmentioning

confidence: 80%

“…For instance, in the root collar and root hair zone high exudation occurs in comparison to root distal parts such as tips (Grayston et al, 1996). Due to different exudation patterns, some sites are better colonized by some rhizobacteria (Gamalero et al, 2004), potentially resulting in spatial differences of bacterial colonization. The amount of photosynthates secreted as root exudates varies also with the type of soil and the availability of nutrients (Kraffczyk et al, 1984;Paterson and Sim, 2000).…”

Section: Chemotaxis Towards Root Exudatesmentioning

confidence: 99%

Plant growth-promoting bacteria in the rhizo- and endosphere of plants: Their role, colonization, mechanisms involved and prospects for utilization

Compant

Clément

Sessitsch

2010

Soil Biology and Biochemistry

1,788

1,013

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In both managed and natural ecosystems, beneficial plant-associated bacteria play a key role in supporting and/or increasing plant health and growth. Plant growth-promoting bacteria (PGPB) can be applied in agricultural production or for the phytoremediation of pollutants. However, because of their capacity to confer plant beneficial effects, efficient colonization of the plant environment is of utmost importance. The majority of plant-associated bacteria derives from the soil environment. They may migrate to the rhizosphere and subsequently the rhizoplane of their hosts before they are able to show beneficial effects. Some rhizoplane colonizing bacteria can also penetrate plant roots, and some strains may move to aerial plant parts, with a decreasing bacterial density in comparison to rhizosphere or root colonizing populations. A better understanding on colonization processes has been obtained mostly by microscopic visualisation as well as by analysing the characteristics of mutants carrying disfunctional genes potentially involved in colonization. In this review we describe the individual steps of plant colonization and survey the known mechanisms responsible for rhizosphere and endophytic competence. The understanding of colonization processes is important to better predict how bacteria interact with plants and whether they are likely to establish themselves in the plant environment after field application as biofertilisers or biocontrol agents.

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“…Some of the bacteria associated with Arbuscular Mycorrhiza (AM) fungi, can improve the mycorrhizal colonization (Hildebrandt et al, 2002), improve root branching (Gamalero et al, 2002), or present antifungal properties (Budi et al, 1999). Since, they share common microhabitats, AM fungi and Plant Growth Promoting Bacteria (PGPBs) must interact during the colonization process and/or as rhizosphere microorganisms (Gamalero et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

Occurrence of Arbuscular Mycorrhizal Fungi and Fusarium in TC Banana Rhizosphere Inoculated with Microbiological Products in Different Soils in Kenya

Onguso¹,

and²,

Njuguini³

et al. 2015

International J. of Soil Science

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The impact of microbiological commercial products (PHC Biopak, Rhizatech and ECO-T) on the occurrence of mycorrhizae and Fusarium in the rhizosphere of tissue culture banana (Gros Mitchel cv.) was assessed. Tissue cultured banana plantlets were inoculated with PHC Biopak (Bacillus), Rhizatech (mycorrhiza) and ECO-T (T. harzianum) under greenhouse conditions using a completely randomized design in a Vertisol, Rhodic Ferralsol and Humic Nitisol sampled from the major banana growing regions in Kenya. Potted plants were later established under field conditions in the three agro ecological zones. Roots and soils sampled at end of potting and at flowering were assessed for AM fungi colonization and Fusarium populations. The effect of product inoculation on AM fungi colonization varied and only significant (p<0.05) in Rhodic Ferralsol with Rhizatech increasing intensity of colonization by 31.9% and PHC Biopak increasing the frequency of colonization by 38.6% compared to the non-inoculated control (12.9%). F. oxysporum, fsp. cubense, F. proliferatum and F. incarnatum were recovered from the experimental soils. Foc was the most abundant in the three soils (prior to inoculation) accounting for 60.6% of all Fusarium colony forming units. After inoculation, at the end of potting stage and at flowering, F. proliferatum was mostly isolated from the three zones accounting for 35.2% of the total fungal population. Foc was isolated from Humic Nitisol and Vertisol accounting for 11.5% of the total fungal population. PHC Biopak, ECO-T and Rhizatech suppressed Foc colony forming units per gram of soil by 47, 68 and 55%, respectively in the Humic Nitisol. ECO-T reduced Fusarium colony forming units per gram of soil by 6% in Rhodic Ferralsol and PHC Biopak by 50% in Vertisol compared to the non-inoculated soils. There is potential in use of commercial microbiological products to suppress Foc and the efficacy of the products depends on soil physico-chemical properties.

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Colonization pattern of primary tomato roots by Pseudomonas fluorescens A6RI characterized by dilution plating, flow cytometry, fluorescence, confocal and scanning electron microscopy

Cited by 89 publications

References 54 publications

Cucumber Rhizosphere Microbial Community Response to Biocontrol Agent Bacillus subtilis B068150

Cucumber Rhizosphere Microbial Community Response to Biocontrol Agent Bacillus subtilis B068150

Plant growth-promoting bacteria in the rhizo- and endosphere of plants: Their role, colonization, mechanisms involved and prospects for utilization

Occurrence of Arbuscular Mycorrhizal Fungi and Fusarium in TC Banana Rhizosphere Inoculated with Microbiological Products in Different Soils in Kenya

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