Colonization of rhizospheres by Bradyrhizobium Sp. in relation to strain persistence and nodulation of some pasture legumes

Bushby, H.V.A.

doi:10.1016/0038-0717(93)90199-l

Cited by 13 publications

(8 citation statements)

References 20 publications

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“…These profiles might be associated with bacterial groups such as growth promoters affected by the rhizosphere and being beneficial to plants (Bushby et al, 1993;Roesti et al, 2006).…”

Section: Resultsmentioning

confidence: 99%

“…For each DNA dilution, PCR amplification was carried out on a final volume of 35 µL, containing 1 µL DNA dilution, PCR buffer (1X), MgCl 2 (1.5 mM), dNTP (0.2 µM of each), bovine serum albumin (0.17 µg µL -1 ), Taq DNA polymerase (0.7 U), and the set of primers 1401-R and 968CG-F (0.2 µM of each). These primers and PCR program were described by Gelsomino et al (1999). The amplified products of each replicate were mixed together in a single microtube.…”

Section: Methodsmentioning

confidence: 99%

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Rhizosphere bacterial communities of potato cultivars evaluated through PCR-DGGE profiles

Ferreira

Dusi

Xavier

et al. 2008

Pesq. agropec. bras.

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-The objective of this work was to determine the shifts on the PCR-DGGE profiles of bacterial communities associated to the rhizosphere of potato cultivars, in order to generate baseline information for further studies of environmental risk assessment of genetically modified potato plants. A greenhouse experiment was carried out with five potato cultivars (Achat, Bintje, Agata, Monalisa and Asterix), cultivated in pots containing soil from an integrated system for agroecological production. The experiment was conducted in a split plot randomized block design with five cultivars, three sampling periods and five replicates. Rhizosphere samples were collected in three sampling dates during plant development. DNA of rhizosphere microorganisms was extracted, amplified by PCR using bacterial universal primers, and analyzed through DGGE. Shifts on the rhizosphere bacterial communities associated to rhizosphere of different cultivars were related to both cultivar and plant age. Differences among rhizosphere bacterial communities were clearest at the earliest plant age, tending to decrease in later stages. This variation was detected among bacterial communities of the five tested cultivars. The characterization of soil microbial communities can be part of plant breeding programs to be used on studies of environmental risk assessment of genetically modified potatoes.Index terms: Solanum tuberosum, associative bacteria, molecular marker, PCA. Comunidades bacterianas associadas à rizosfera de cultivares de batata avaliadas por perfis de PCR-DGGEResumo -O objetivo deste trabalho foi determinar as alterações nos perfis de PCR-DGGE das comunidades bacterianas associadas à rizosfera de cultivares de batata, para obter informações para futuros estudos de avaliação de risco ambiental de plantas de batatas geneticamente modificadas. Foi conduzido experimento em casa de vegetação com cinco cultivares de batata (Achat, Bintje, Ágata, Monalisa e Asterix), cultivadas em vasos com solo de um sistema integrado de produção agroecológica. O delineamento experimental foi o de blocos ao acaso, em parcelas subdivididas, com cinco cultivares, três períodos amostrais e cinco repetições. As amostras de rizosfera foram coletadas em três diferentes épocas durante o desenvolvimento das plantas. O DNA dos microrganismos associados à rizosfera foi extraído, amplificado por PCR com uso de iniciadores universais para bactérias e analisados por DGGE. Foram observadas alterações, relacionadas à cultivar e à idade da planta, nos perfis das comunidades bacterianas associadas à rizosfera das diferentes cultivares. As diferenças entre as comunidades bacterianas foram maiores na fase inicial do crescimento das plantas, com tendência a diminuir no estágio final de desenvolvimento. Essa variação foi detectada na comunidade bacteriana das cinco cultivares estudadas. A caracterização da microbiota do solo pode ser parte de programas de melhoramento de plantas a ser utilizada em estudos de avaliação de risco ambiental de batatas geneticamente modificadas...

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“…These profiles might be associated with bacterial groups such as growth promoters affected by the rhizosphere and being beneficial to plants (Bushby et al, 1993;Roesti et al, 2006).…”

Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Rhizosphere bacterial communities of potato cultivars evaluated through PCR-DGGE profiles

Ferreira

Dusi

Xavier

et al. 2008

Pesq. agropec. bras.

View full text Add to dashboard Cite

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“…Frequently, introduced strains are outnumbered by as much as 250 to 1 , are not evenly distributed throughout the soil (Bushby' 1993;McDermott and Graham, 1989), and are often not well adapted to general soil conditions. As a result, inoculant rhizobia may only form 5-10% of the nodules produced in the first year, with the frequency with which they are found in nodules declining even further in subsequent crops.…”

Section: Competition In Soil and Rhizospherementioning

confidence: 99%

Soil Biology of the Rhizobiaceae

Sadowsky¹,

Graham²

1998

The Rhizobiaceae

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“…IGS III was predominant in both legumes and was distributed in all the treatments applied to cowpea and green gram. The dominance of indigenous strains can be attributed to a number of factors; the adaptability of indigenous rhizobia to their environment results in high levels of saprophytic competence (Zengeni et al 2006); build up of background strains in the presence of the host rhizosphere (Dowling and Broughton 1986;Bushby 1993;Mendes and Bottomley 1998) and the competitive ability of background strains for sites of infection on the host plant roots and nodulation (Laguerre et al 2003;Leite et al 2009). It is therefore imperative to consider how rhizobial populations in the soil can be manipulated to influence, with or without effectual inoculation, legume nodulation, N 2 fixation and plant productivity (Brockwell et al 1995;McInnes and Haq 2003).…”

mentioning

confidence: 99%

Potential of indigenous bradyrhizobia versus commercial inoculants to improve cowpea (Vigna unguiculataL. walp.) and green gram (Vigna radiataL. wilczek.) yields in Kenya

Mathu

Herrmann

Pypers

et al. 2012

Soil Science and Plant Nutrition

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Limited information is available on reduced cowpea (Vigna unguiculata L. Walp.) and green gram (Vigna radiata L.Wilczek.) yields in Kenya. Declining soil fertility and absence or presence of ineffective indigenous rhizobia in soils are assumptions that have been formulated but still require to be demonstrated. In this study, soils were collected from legume growing areas of Western (Bungoma), Nyanza (Bondo), Eastern (Isiolo), Central (Meru) and Coast (Kilifi) provinces in Kenya to assess indigenous rhizobia in soils nodulating cowpea and green gram under greenhouse conditions. Our results showed that highest nodule fresh weights of 4.63 and 3.32 g plant À1 for cowpea and green gram were observed in one soil from Isiolo and another from Kilifi, respectively, suggesting the presence of significant infective indigenous strains in both soils. On the other hand, the lowest nodule fresh weights of 2.17 and 0.72 g plant À1 were observed in one soil from Bungoma for cowpea and green gram, respectively. Symbiotic nitrogen (N) fixation by cowpea and green gram was highest in Kilifi soil with values of 98% and 97%, respectively. A second greenhouse experiment was undertaken to evaluate the performance of commercial rhizobial inoculants with both legumes in Chonyi soil (also from Coast province) containing significant indigenous rhizobia [>13.5 Â 10 3 Colony Forming Units (CFU) g À1]. Rhizobial inoculation did not significantly (P < 0.05) affect nodulation, biomass yield and shoot N content in cowpea and green gram compared with controls. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) of the 16S-23S rDNA intergenic spacer (IGS) region analysis of nodules revealed six groups of which only IGS Group IV corresponded with those from commercial inoculants applied, indicating a lower competitiveness of inoculated strains. In cowpea, IGS III was dominant in nodules of plants inoculated with Biofix and Rizoliq commercial inoculants, and the uninoculated control treatment (63.2, 60 and 52.9%, respectively). Similarly, in green gram, IGS Group III was dominant in nodules of plants inoculated with Biofix 704 and Rizoliq commercial inoculants, and the uninoculated control treatment (75, 73.7 and 61.1%, respectively). Our results suggest that the systematic inoculation of both legumes with current available commercial inoculants to improve biomass yields is not necessary in these regions of Kenya. Also, according to our study, it would make sense to promote the utilization of indigenous strains performing well with both legumes.

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Colonization of rhizospheres by Bradyrhizobium Sp. in relation to strain persistence and nodulation of some pasture legumes

Cited by 13 publications

References 20 publications

Rhizosphere bacterial communities of potato cultivars evaluated through PCR-DGGE profiles

Rhizosphere bacterial communities of potato cultivars evaluated through PCR-DGGE profiles

Soil Biology of the Rhizobiaceae

Potential of indigenous bradyrhizobia versus commercial inoculants to improve cowpea (Vigna unguiculataL. walp.) and green gram (Vigna radiataL. wilczek.) yields in Kenya

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