Colonization of Enteroaggregative Escherichia coli and Shiga toxin-producing Escherichia coli in chickens and humans in southern Vietnam

Trung, Nguyen Vinh; Nhung, Hoang Ngoc; Carrique-Mas, Juan; Huynh, Ho Phi; Tuyen, Ha Thanh; Campbell, James; Nhung, Nguyen Thi; Minh, Pham Van; Wagenaar, Jaap A.; Nhu, Nguyen Thi Khanh; Hieu, Thai Quoc; Schultsz, Constance; Hoa, Ngô Thị

doi:10.1186/s12866-016-0827-z

Cited by 13 publications

(7 citation statements)

References 29 publications

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“…The present study correlates with other studies where the detection rate of EHEC pathotype among children under the age of 5 years was high 61 . It also agrees with studies that used an equal number of virulence genes to characterise the EHEC pathotype 16,41 . The overall prevalence of EPEC/EHEC (eaeA gene) found in our study is consistent with other studies 18,21,41 .…”

Section: Discussion Identification and Distribution Of Dec Pathotypessupporting

confidence: 88%

“…It also agrees with studies that used an equal number of virulence genes to characterise the EHEC pathotype 16,41 . The overall prevalence of EPEC/EHEC (eaeA gene) found in our study is consistent with other studies 18,21,41 . A higher frequency of 42.3% has been reported in India 15 while over 50% has been reported in South African domestic water 6 , 67% in Tennessee 62 and 47.5% found in Iranian children 7 .…”

Section: Discussion Identification and Distribution Of Dec Pathotypessupporting

confidence: 88%

“…Drinking poorly treated or untreated polluted water or consuming raw or partiallycooked meat and vegetables have been implicated in the transmission of pathogenic E. coli strains to humans 41,42 . Molecular techniques like the enterobacterial repetitive intergenic consensus-PCR (ERIC-PCR) have been used to detect similarity between Gram-negative bacteria by comparing their genetic fingerprints 40,43 .…”

Section: Introductionmentioning

confidence: 99%

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Antibiotic Resistance Profile and Clonality of E. coli Isolated from Water and Paediatric Stool Samples in the North-West, Province South Africa

Chukwu¹,

Abia²,

Ubomba-Jaswa³

et al. 2019

J Pure Appl Microbiol

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This study investigated the antibacterial resistance profiles of E. coli pathotypes isolated from children under five years and drinking water samples collected from the North West Province of South Africa and ascertained the clonality of the isolates. Two hundred and forty-one E. coli isolates were recovered from stool samples of diarrhoeic and non-diarrhoeic children under five years old, and drinking water, using the Colilert-18 ® Quanti-tray/2000 and Eosin methylene blue agar. The presence of enteropathogenic (eaeA), enterohaemorrhagic (eaeA,stx1, stx2 and flicH7), enteroaggregative (eagg), enteroinvasive (ipaH) and enterotoxigenic (ST and LT) E. coli pathotypes were also investigated using PCR. Antibiotic susceptibility was carried out through the disk diffusion method. The presence of blaCTX-M, blaSHV, blaCMY, and blaDHA genes that code for β-lactamases was investigated using real-time PCR. Similarities between human and water isolates were tested using ERIC-PCR. Overall, EHEC (35.8%), EPEC/EHEC (22%), ETEC (21.6%) and EIEC (20.2%) were detected. The highest antibiotic resistance was detected to Clarithromycin (100%) and Erythromycin (100%) while the lowest resistance was against Gentamicin (0.4%). Also, 100% sensitivity was recorded to imipenem and meropenem. Multi-antibiotic resistance was observed in all the pathotypes, and the ESBL genes were detected in 71.6% of the pathotypes. ERIC-PCR indicated 100% similarities in some water and human samples. Pathogenic E. coli is amongst the diarrhoea-causing agents in the North West Province with EHEC being the most identified pathotype. The clonal relatedness of the human and water isolates suggests that domestic water might be a route of transmission.

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Section: Discussion Identification and Distribution Of Dec Pathotypessupporting

confidence: 88%

Section: Discussion Identification and Distribution Of Dec Pathotypessupporting

confidence: 88%

Section: Introductionmentioning

confidence: 99%

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Antibiotic Resistance Profile and Clonality of E. coli Isolated from Water and Paediatric Stool Samples in the North-West, Province South Africa

Chukwu¹,

Abia²,

Ubomba-Jaswa³

et al. 2019

J Pure Appl Microbiol

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“…The acquisition of pAA plasmids by E. coli ST131 is interesting, considering that the ST131 lineage is thought of as a host generalist ( 39 ), whereas EAEC appears to be highly adapted to humans, without a natural animal reservoir ( 40, 41 ). However, the EAEC ST131 lineage described here is reminiscent of the UTI outbreak in Copenhagen in 1991 caused by an E. coli O78:H10 clonal group in that both fulfilled the molecular criteria for EAEC and contained multiple ExPEC virulence genes ( 32 ).…”

Section: Discussionmentioning

confidence: 99%

Emergence of enteroaggregativeEscherichia coliwithin the ST131 lineage as a cause of extraintestinal infections

Boll

Stegger

Hasman

et al. 2018

Preprint

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26Escherichia coli sequence type 131 (ST131) is a major cause of urinary and bloodstream infections 27 and its association with extended-spectrum β-lactamases (ESBL) significantly complicates treatment. 28 Most notorious is its rapidly expanding H30-Rx clade (named for containing allele 30 of the type-1 29 fimbrial adhesin gene fimH and extensive antimicrobial resistance), which appears to have emerged 30 55 Escherichia coli sequence type 131 (ST131) is the dominant multidrug-resistant (MDR) 56 extraintestinal pathogenic E. coli (ExPEC) lineage worldwide, causing a wide range of infections 57 including bloodstream and urinary tract infections (BSIs/UTIs) (1-3). Its rise to global dominance 58 and pathogenicity is thought to have been primed by sequential acquisition of virulence-associated 59 genes followed by development of antibiotic resistance (4, 5). ST131 predominantly exhibits 60 serotype O25:H4 and is closely associated with fluoroquinolone resistance and the production of the 61 CTX-M-15 extended-spectrum β-lactamase (ESBL) (2, 6). The expansion of E. coli ST131 in the 62 United States has been driven mainly by a single clade, designated H30 because of its tight 63 association with allele 30 of the type-1 fimbrial adhesin gene, fimH. H30 has a prominent MDR-64 associated clade, H30R, which accounts for most fluoroquinolone resistance within ST131. H30R in 65 turn has two main sub-clades: H30R, which accounts for almost all ST131-associated CTX-M-14 66 and CTX-M-27 production (although most members are ESBL-negative), and H30Rx, which 67 accounts for almost all ST131-associated CTX-M-15 production (6). In addition to H30, other 68 distinct clades of ST131 are also circulating worldwide, most commonly associated to the 22 and 41 69 fimH alleles. While these non-H30 allelic variants are normally not associated with carriage of CTX-70 M-genes, cases of bla CTX-M-14 , bla CTX-M-15 and bla CTX-M-27 acquisition by ST131 lineages carrying 71 fimH22 or -H41 have been reported (4, 7). 72 ST131 isolates typically carry multiple ExPEC-associated virulence genes encoding 73 adhesins, toxins, and siderophores, whereas virulence genes typical of diarrheagenic E. coli (DEC) 74rarely have been reported (8, 9). However, we recently surveyed an international World Health 75 Organization (WHO) collection of historic E. coli for ST131 O25 isolates, assessing them for 76 temporal trends of antibiotic resistance and virulence traits. Among a total of 128 ST131 isolates we 77 found 12 (9%) that fulfilled molecular criteria for the enteroaggregative E. coli (EAEC) pathotype 78 (10). Pulsed-field gel electrophoresis (PFGE) analysis revealed a cluster comprising seven of these 79 6 EAEC isolates. Of these, sixincluding two urine isolates from patients with UTI, three fecal 80 isolates from patients with diarrhea, and one lower respiratory tract isolate (associated symptoms 81 unknown)were from Danish patients (1998)(1999)(2000), supporting the occurrence of an unrecognized 82 EAEC ST131-associated outbreak of UTI and po...

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“…Remarkably, this is the first study to report these combinations in waterborne DEC isolates. Other studies reported a different combination of genes from both EAEC and EHEC [ 43 , 46 ]. This finding is of a public health concern as mixed infections usually involve more dehydration compared with episodes caused by a single DEC pathotype.…”

Section: Discussionmentioning

confidence: 99%

Prevalence, diversity of diarrhoeagenic Escherichia coli and associated risk factors in well water in Ile-Ife, Southwestern Nigeria

2022

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Background Diarrhoeagenic Escherichia coli (DEC) strains are common causes of morbidity and mortality worldwide. Waterborne DEC could pose a health risk to humans through domestic use of contaminated water. However, epidemiological studies on DEC in well water are scarce in Nigeria. This study determined the prevalence, diversity and factors associated with the presence of DEC in well water in Ile-Ife, southwestern Nigeria. Methods We assessed 143 wells for safety and a questionnaire was administered. Contaminating isolates were identified as E. coli by amplifying their 16S rRNA gene. Five diarrhoeagenic E. coli pathotypes were sought using multiplex polymerase chain reaction (PCR). (GTG)5 repetitive PCR and Shannon diversity index were used to determine isolates diversity. Multivariate analysis was used to reveal the factors associated with the presence of DEC in well water. Results Fifty-six (39.2%) wells were contaminated by diarrhoeagenic E. coli. Wells with dirty platforms, undercut by erosion and sited near septic tanks significantly harboured DEC (p < 0.05). There was a preponderance of Shiga-toxin producing E. coli among the isolates with 10 (17.9%) wells contaminated by multiple DEC. The DEC isolates showed 45 unique fingerprints and were divided into six clades, with an overall diversity index of 18.87. Discussion The presence of DEC in well water highlights the risk to human health associated with the use of untreated water. There was a high degree of genetic diversity among the isolates implying multiple sources of contamination. There is a need for periodic sanitation and inspection of wells for cracks to prevent seepages and possible outbreaks of waterborne diseases.

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Colonization of Enteroaggregative Escherichia coli and Shiga toxin-producing Escherichia coli in chickens and humans in southern Vietnam

Cited by 13 publications

References 29 publications

Antibiotic Resistance Profile and Clonality of E. coli Isolated from Water and Paediatric Stool Samples in the North-West, Province South Africa

Antibiotic Resistance Profile and Clonality of E. coli Isolated from Water and Paediatric Stool Samples in the North-West, Province South Africa

Emergence of enteroaggregativeEscherichia coliwithin the ST131 lineage as a cause of extraintestinal infections

Prevalence, diversity of diarrhoeagenic Escherichia coli and associated risk factors in well water in Ile-Ife, Southwestern Nigeria

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