Colonization of albumin-producing hepatocytes derived from transplanted F344 rat bone marrow cells in the liver of congenic Nagase's analbuminemic rats

Arikura, Jun; Inagaki, Masaru; Xiang, Huiling; Ozaki, Atsuko; Onodera, Kenji; Ogawa, Katsuhiro; Kasai, S.

doi:10.1016/j.jhep.2004.04.020

Cited by 18 publications

(28 citation statements)

References 31 publications

(23 reference statements)

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“…Arikura and his colleagues [25] immediately injected normal BM-MSCs through the portal vein after 70% partial hepatectomy of albumin-deficient rats. Four weeks after the injections, the expression of albumin mRNA and protein was seen in the liver of the albumin-deficient rats.…”

Section: Discussionmentioning

confidence: 99%

Bone Marrow-Derived Mesenchymal Stem Cells Promote Hepatic Regeneration after Partial Hepatectomy in Rats

Zhang

et al. 2013

Pathobiology

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Objectives: Our goal was to study the ability of mesenchymal stem cells (MSCs) to stimulate liver regeneration after partial hepatectomy in rats. Methods: MSCs were isolated from bone marrow and cultured in vitro. Their characteristics were analyzed by flow cytometry. After 70% partial hepatectomy, Sprague-Dawley rats were randomly divided into three groups: a control group that was injected with saline, animals that received bone marrow-derived MSCs (BM-MSCs) by tail vein injection (the BM-MSC-TV group) and animals that received BM-MSCs by portal vein injection (the BM-MSC-PV group). The injected BM-MSCs were traced by labeling with 4′,6-diamidino-2-phenylindole, and cell proliferations were determined by immunohistochemical staining with Ki-67 and 5-bromo-2′-deoxyuridine. Results: After the third passage, the cultured BM-MSCs had a fibroblast-like morphology and expressed high levels of stem cell markers CD29 and CD90. The levels of albumin rose significantly in the BM-MSC-TV and BM-MSC-PV groups compared with the control group. The number of 4′,6-diamidino-2-phenylindole-positive liver cells in the BM-MSC-PV group was significantly higher than in the BM-MSC-TV group. The levels of Ki-67 and 5-bromo-2′-deoxyuridine were significantly higher in the BM-MSC-TV and the BM-MSC-PV groups than in the controls. Conclusion: Taken together, these results indicate that BM-MSC injections enhance liver regeneration after partial hepatectomy in rats.

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Section: Discussionmentioning

confidence: 99%

Bone Marrow-Derived Mesenchymal Stem Cells Promote Hepatic Regeneration after Partial Hepatectomy in Rats

Zhang

et al. 2013

Pathobiology

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“…FAH (−/−) mouse is an animal model of fatal hereditary tyrosinemia type I, when this model was irradiated followed by transplantation with unfractionated BMSCs or purified HSCs, both of which can give rise to hepatocytes, tyrosinemia can be improved [37]. Nagase analbuminemic rats (F344alb) is an inherited model for albumin production deficiency, when BMSCs from wild-type rats were infused via the portal vein into the F344alb model pretreated with 70% hepatectomy, clusters of alb+ hepatocytes and albumin mRNA were detected in recipient liver, with elevated serum albumin level [69]. These two independent experiments indicate the BMSCs can be applied in the correction of inherited liver diseases.…”

Section: Gene Therapymentioning

confidence: 99%

“…Clusters of albumin positive Fig. 2 Potential delivery approaches of bone marrow stem cells for liver diseases hepatocytes were detected in livers of the recipients, indicating that BMSCs infusion via portal vein can increase clusters of albumin-producing hepatocytes within the liver of analbuminemic rats [69].…”

Section: Portal Vein Injectionmentioning

confidence: 99%

Therapeutic Potential of Adult Bone Marrow Stem Cells in Liver Disease and Delivery Approaches

Liu

2008

Stem Cell Rev

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Hematopoietic stem cells (HSCs) and mesenchymal stem cell (MSCs) are two main subtypes of bone marrow stem cells. Extensive studies have been carried out to investigate the therapeutic potential of BMSCs in liver disease. A number of animal and human studies demonstrated that either HSCs or MSCs could be applied to therapeutic purposes in certain liver diseases. The diseased liver may recruit migratory stem cells, particularly from the bone marrow, to generate hepatocyte-like cells either by transdifferentiation or cell fusion. Transplantation of BMSCs has therapeutic effects of restoration of liver mass and function, alleviation of fibrosis and correction of inherited liver diseases. There are still controversial results over the potential effects of BMSCs on liver diseases, and some of the discrepancies are thought to be lied in the differences of experimental protocols, differences in individual research laboratory, and the uncertainties of the techniques employed. Several potential approaches for BMSCs delivery in liver diseases have been proposed in animal studies and human trials. BMSCs can be delivered via intraportal vein, systemic infusion, intraperitoneal, intrahepatic, intrasplenic. The optimal stem cells delivery should be easy to perform, less invasive and traumatic, minimum side effects, and with high cells survival rate. In this review, we focus on the up-to-date evidence of therapeutic effects of BMSCs on liver disease, the characteristics of various delivery approaches, and the considerations for future studies.

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“…Our previous studies have demonstrated that the transplantation model using Fischer 344 rats (F344) and F344 congenic Nagase's analbuminemic rats (F344alb) to be useful for the efficiency of repopulation of the hepatocytes after HT-Tx and BM-Tx in the liver [4,25,26]. F344alb have the genetic background of F344 and are otherwise normal except for a seven base pair deletion downstream of the exon H splice site within the ninth intron of albumin gene, which leads to the inability of hepatocytes to produce albumin [27].…”

Section: Introductionmentioning

confidence: 99%

Effects of Bone Marrow and Hepatocyte Transplantation on Liver Injury

Zhang

Inagaki

Jiang

et al. 2009

Journal of Surgical Research

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Colonization of albumin-producing hepatocytes derived from transplanted F344 rat bone marrow cells in the liver of congenic Nagase's analbuminemic rats

Cited by 18 publications

References 31 publications

Bone Marrow-Derived Mesenchymal Stem Cells Promote Hepatic Regeneration after Partial Hepatectomy in Rats

Bone Marrow-Derived Mesenchymal Stem Cells Promote Hepatic Regeneration after Partial Hepatectomy in Rats

Therapeutic Potential of Adult Bone Marrow Stem Cells in Liver Disease and Delivery Approaches

Effects of Bone Marrow and Hepatocyte Transplantation on Liver Injury

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