Bone Marrow-Derived Mesenchymal Stem Cells Promote Hepatic Regeneration after Partial Hepatectomy in Rats

Abstract

Objectives: Our goal was to study the ability of mesenchymal stem cells (MSCs) to stimulate liver regeneration after partial hepatectomy in rats. Methods: MSCs were isolated from bone marrow and cultured in vitro. Their characteristics were analyzed by flow cytometry. After 70% partial hepatectomy, Sprague-Dawley rats were randomly divided into three groups: a control group that was injected with saline, animals that received bone marrow-derived MSCs (BM-MSCs) by tail vein injection (the BM-MSC-TV group) and animals that received BM-MSCs by portal vein injection (the BM-MSC-PV group). The injected BM-MSCs were traced by labeling with 4′,6-diamidino-2-phenylindole, and cell proliferations were determined by immunohistochemical staining with Ki-67 and 5-bromo-2′-deoxyuridine. Results: After the third passage, the cultured BM-MSCs had a fibroblast-like morphology and expressed high levels of stem cell markers CD29 and CD90. The levels of albumin rose significantly in the BM-MSC-TV and BM-MSC-PV groups compared with the control group. The number of 4′,6-diamidino-2-phenylindole-positive liver cells in the BM-MSC-PV group was significantly higher than in the BM-MSC-TV group. The levels of Ki-67 and 5-bromo-2′-deoxyuridine were significantly higher in the BM-MSC-TV and the BM-MSC-PV groups than in the controls. Conclusion: Taken together, these results indicate that BM-MSC injections enhance liver regeneration after partial hepatectomy in rats.

Mesenchymal Stem Cells Transplantation following Partial Hepatectomy: A New Concept to Promote Liver Regeneration—Systematic Review of the Literature Focused on Experimental Studies in Rodent Models

“…Thus, they may be ideal seed cells for the treatment of injured tissue. The use of BM-MSCs is also being explored in the fields of regenerative medicine and tissue engineering (Table 3) [20]. …”

“…From these studies, in five, authors performed ADSCs transplantation [14, 17–19, 21], whereas, in two, authors transplanted BM-MSCs [16, 20] (Table 1). In all included papers on MSCs transplantation following PH, authors used rats as experiment animals, except from the study of Saito et al [19], in which authors have performed their experiments in mice.…”

“…Among included and reviewed studies for the aims of this article, only two studies were retrieved in which authors transplanted BM-MSCs, following PH in rats [16, 20]. …”

Cross Effects of Resveratrol and Mesenchymal Stem Cells on Liver Regeneration and Homing in Partially Hepatectomized Rats

“…Abdel Aziz et al showed that bone marrow (BM) mesenchymal stem cells (MSCs) could differentiate into hepatocytelike cells and reduced fibrosis by decreasing the precipitation of collagen [6]. Li et al have found that MSCs promote hepatic regeneration after partial hepatectomy and there was no positive correlation between liver regeneration and the amount of homing of these BM-MSCs [7].…”

Effect of Multipotent Stromal Cells on the Function of Cell Mitochondria in Regenerating Liver

“…Stimulation of reparative processes in the damaged liver under the effect of transplanted MSC has been demonstrated not once [10]. This effect can be mediated by several mechanisms.…”