Colonic mucin synthesis is increased by sodium butyrate.

Finnie, I A; Dwarakanath, A D; Taylor, Ben; Rhodes, Jonathan M.

doi:10.1136/gut.36.1.93

Cited by 197 publications

(165 citation statements)

References 29 publications

(3 reference statements)

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“…Barcelo et al [23] demonstrated a significant discharge of mucins at concentrations of 5 mM of butyrate, while increasing the concentration to 100 mM decreased this mucus response in mice. Highly relevant, ex vivo stimulation of macroscopically normal fresh colon tissue with 0.05-1 mM butyrate stimulates MUC2 synthesis, whereas at stimulation with 10 mM butyrate MUC2 synthesis levels returned to basal levels [24]. These studies correlate with our data with respect to the effects of butyrate on MUC2 expression (i.e.…”

Section: Discussionsupporting

confidence: 81%

“…The mucin MUC2 is the predominant mucin in the colon and MUC2 synthesis is diminished in UC [19,20] and presumably also NEC. It has been shown in cell line studies, experimental animal models and fresh human intestinal tissue specimens, that butyrate alters MUC2 expression in a dose dependent manner [21][22][23][24][25]. However, the mechanisms that are responsible for these alterations have not been studied in detail so far.…”

Section: Introductionmentioning

confidence: 99%

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218 The Regulation of the Intestinal Mucin MUC2 Expression By Short Chain Fatty Acids: Implications for Epithelial Protection

Paassen¹,

Vincent²,

Puiman³

et al. 2009

Gastroenterology

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Section: Discussionsupporting

confidence: 81%

Section: Introductionmentioning

confidence: 99%

218 The Regulation of the Intestinal Mucin MUC2 Expression By Short Chain Fatty Acids: Implications for Epithelial Protection

Paassen¹,

Vincent²,

Puiman³

et al. 2009

Gastroenterology

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“…The protective effect of the mucus is related to the presence of mucins, which are predominantly glycoproteins in their composition. In addition to providing mechanical protection, they present bactericide properties 7,8 . Breakage of the protective barrier or mucus, as occurs in inflammatory intestinal diseases, allows invasion of bacteria through the epithelial surface 7,8,19 .…”

Section: Discussionmentioning

confidence: 99%

“…It has already been demonstrated that the capacity of the colon epithelium for mucin synthesis depends on adequate supply of SCFAs 7 . Recent studies have shown the importance of maintaining the supply of SCFAs, particularly butyrate, in order to have adequate gene expression relating to mucin formation 8,9 .…”

Section: Introductionmentioning

confidence: 99%

“…Most of the protective effect provided by the mucous layer relates to the presence of mucins, which are the predominant glycoproteins in the chemical composition of this layer 6,8 . Briefly, the mucosa of the digestive tract produces two main types of mucins, represented by neutral and acid mucins 7 . Acid mucins are composed of sulfomucins and sialomucins 6 .…”

Section: Introductionmentioning

confidence: 99%

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Quantification by computerized morphometry of tissue levels of sulfomucins and sialomucins in diversion colitis in rats

et al. 2010

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PURPOSE: To quantify the intensity of sulfomucin and sialomucin expression in the colon mucosa, by means of computer-assisted image processing, comparing segments with and without fecal stream and correlating with the duration of fecal transit exclusion. METHODS: Forty-five Wistar rats were subjected to diversion of the fecal stream in the left colon by means of constructing a proximal colostomy and distal mucosal fistula. They were distributed randomly into three experimental groups of 15 animals, of which 10 were subjected to colon diversion (experimental subgroup) and five were only subjected to laparotomy, without colon diversion (control subgroup). The three experimental groups were formed according to the sacrifice date, which was to be performed six weeks after the surgical procedure (Group A), 12 weeks (Group B) and 18 weeks (Group C). The sulfomucin and sialomucin expression in the colon mucosa was evaluated using the histochemical technique of high iron diamine-alcian blue (HID-AB). The tissue expression was quantified for each animal, in the segments with and without fecal stream, at a location where there were four complete contiguous crypts in two random fields, with the aid of the computer-assisted image analysis software. The final value was taken to be the mean reading from the two fields selected, in the segments with and without fecal stream. To compare the expressions of the two mucin subtypes in the segments with and without fecal stream, the paired Student t test was used. To analyze variance according to duration of exclusion, ANOVA with the Newman-Keuls post-test was used, setting the significance level at 5% (p<0.05). RESULTS: There were significant reductions in tissue sulfomucin and sialomucin content in the colon without fecal stream, independent of the duration of exclusion considered. There was increased tissue sulfomucin content and decreased tissue sialomucin in the segments without fecal stream, with increasing duration of exclusion. CONCLUSIONS: Diversion of the fecal transit decreased the tissue sulfomucin and sialomucin content in the segments without fecal stream. Notwithstanding the reduction in the levels of both subtypes of acid mucin in the segments without fecal stream, there was increased tissue sulfomucin content and decreased tissue sialomucin with increasing duration of intestinal diversion.

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Mannose‐Decorated Co‐Polymer Facilitates Controlled Release of Butyrate to Accelerate Chronic Wound Healing

Lauterbach

Slezak

Wang

et al. 2023

Adv Healthcare Materials

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Butyrate is a key bacterial metabolite that plays an important and complex role in modulation of immunity and maintenance of epithelial barriers. Its translation to clinic has been limited by poor bioavailability, pungent smell, and the need for high doses, and effective delivery strategies have yet to realize clinical potential. Here we report a novel polymeric delivery platform for tunable and sustainable release of butyrate consisting of a methacrylamide backbone with butyryl ester or phenyl ester side chains as well as mannosyl side chains, which is also applicable to other therapeutically‐relevant metabolites. We explore this platform's utility in the treatment of non‐healing diabetic wounds. This butyrate‐containing material modulated immune cell activation in vitro and induced striking changes in the milieu of soluble cytokine and chemokine signals present within the diabetic wound microenvironment in vivo. This novel therapy shows efficacy in the treatment of non‐healing wounds through the modulation of the soluble signals present within the wound, and importantly accommodates the critical temporal regulation associated with the wound healing process. Currently, the few therapies to address non‐healing wounds demonstrate limited efficacy. Our novel platform is positioned to address this large unmet clinical need and improve the closure of otherwise non‐healing wounds.This article is protected by copyright. All rights reserved

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Colonic mucin synthesis is increased by sodium butyrate.

Cited by 197 publications

References 29 publications

218 The Regulation of the Intestinal Mucin MUC2 Expression By Short Chain Fatty Acids: Implications for Epithelial Protection

218 The Regulation of the Intestinal Mucin MUC2 Expression By Short Chain Fatty Acids: Implications for Epithelial Protection

Quantification by computerized morphometry of tissue levels of sulfomucins and sialomucins in diversion colitis in rats

Mannose‐Decorated Co‐Polymer Facilitates Controlled Release of Butyrate to Accelerate Chronic Wound Healing

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