Colocalization of Nestin and Vimentin/Desmin in Skeletal Muscle Cells Demonstrated by Three-Dimensional Fluorescence Digital Imaging Microscopy

Sjöberg, Gunnar; Jiang, Wei‐Qin; Ringertz, Nils R.; Lendahl, Urban; Sejersen, Thomas

doi:10.1006/excr.1994.1281

Cited by 105 publications

(77 citation statements)

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“…This indicates that these two IF proteins form copolymers. Copolymerization between nestin and class III IF proteins has been discussed in earlier studies (1,8) and is supported by the findings that neither nestin nor vimentin can form filaments in GFAP-deficient astrocytes without the presence of a coexpressed IF network (9). The ability of nestin to coassemble with vimentin in vitro has recently been shown (10).…”

Section: Discussionmentioning

confidence: 58%

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Mitotic Reorganization of the Intermediate Filament Protein Nestin Involves Phosphorylation by cdc2 Kinase

Sahlgren

Mikhailov

Hellman

et al. 2001

Journal of Biological Chemistry

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109

112

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as a cdc2-specific phosphorylation site on nestin. We generated a phosphospecific nestin antibody recognizing the phosphorylated form of this site. By using this antibody we observed that nestin shows constitutive phosphorylation at Thr 316 , which is increased during mitosis. This study shows that nestin is reorganized during mitosis and that cdc2-mediated phosphorylation is an important regulator of nestin organization and dynamics during mitosis.

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Section: Discussionmentioning

confidence: 58%

“…In these cells, nestin shows a similar intracellular organization as the class III IF proteins (1,8). Based on these observations, a possible copolymerization of nestin and the class III IF proteins, has been postulated.…”

Section: The Intermediate Filament (If)mentioning

confidence: 78%

Mitotic Reorganization of the Intermediate Filament Protein Nestin Involves Phosphorylation by cdc2 Kinase

Sahlgren

Mikhailov

Hellman

et al. 2001

Journal of Biological Chemistry

Self Cite

109

112

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“…22,30 Nonetheless, nestin-positive cells can be found in adult tissues and may function as reserve cells that can be reactivated to proliferate and migrate in response to injury. 22 The fact that nestin is expressed in the mature podocyte is an intriguing observation given that these cells are usually considered to be nonproliferating.…”

Section: Discussionmentioning

confidence: 99%

Podocytes Contribute to the Formation of Glomerular Crescents

Thorner¹,

Ho²,

Eremina³

et al. 2008

Journal of the American Society of Nephrology

115

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The cellular composition of crescents in glomerular disease is controversial. The role of podocytes in crescent formation has been especially difficult to study because podocytes typically lose their characteristic terminally differentiated phenotype under disease conditions, making them difficult to identify. We reasoned that the intermediate filament protein nestin, a marker of progenitor cells that has recently been identified in podocytes, may allow the investigation of podocyte involvement in glomerular crescents. In a series of 35 biopsies with crescentic glomerular disease, all showed nestin-positive cells in the crescents, ranging in number from occasional to approximately 50% of crescent cells. Other podocyte markers, such as podocin and WT1, failed to identify cells in crescents, and no contribution by endothelial or myogenic cells was noted. CD68-positive cells were observed in 80% of cases but were never as numerous as the nestin-positive cells. Nestin and CD68 were not coexpressed by the same cells, providing no evidence of trans-differentiation of podocytes into a macrophage phenotype. Keratinpositive cells were found in crescents in 51% of cases, but only as occasional cells. Up to one third of crescent cells were cycling in 48% of biopsies, and double immunostaining identified these cells as a mixture of nestin-positive cells and "null" cells (negative for nestin, CD68, and keratin). In addition to our observations in human disease, we also identified nestin-positive proliferating podocytes in the crescents of 2 mouse models of crescentic glomerulonephritis. We conclude that podocytes play a role in the formation of glomerular crescents.

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“…13,14 Subsequently, nestin was found to be expressed by immature glial cells, 16 astrocytes in injured central nervous system, 17,18 primitive neuroectodermal tumors and gliomas, 16,19 melanomas, 20 myoblasts, 21 and immature cardiac-and skeletal-muscle cells. 15,22,23 Stellate cells in normal rat liver contain GFAP, [7][8][9][10] an intermediate filament protein characteristic of astrocytes. In addition to this cytoskeletal protein, stellate cells express, on their surface, the cell adhesion molecule N-CAM.…”

Section: Discussionmentioning

confidence: 99%

“…In spite of the relatively low sequence homology, nestin forms heteropolymers with type III filament proteins. 15 In addition to neural precursor cells, nestin is expressed by immature glial cells, 16 reactive astrocytes, 17,18 primitive neuroectodermal tumors, 16,19 and melanomas. 20 Also, immature cardiac 21 and skeletal muscle cells 15,22,23 express nestin before terminal differentiation.…”

mentioning

confidence: 99%

Class VI intermediate filament protein nestin is induced during activation of rat hepatic stellate cells

Niki¹,

Pekny

Hellemans³

et al. 1999

Hepatology

259

169

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Hepatic stellate cells are considered to be liver-specific pericytes that play a key role in liver fibrosis. Because these cells express desmin and smooth muscle ␣-actin, they were assumed to be of myogenic origin. This hypothesis became doubtful when it was reported that stellate cells also express glial fibrillary acidic protein and neural cell adhesion molecule. In the present study, we show that activated stellate cells express nestin, a class VI intermediate filament protein originally identified as a marker for neural stem cells. Expression of nestin was first studied during spontaneous activation of stellate cells in culture. Immunohistochemistry showed that nestin-positive stellate cells already appeared at day 3, and nearly all the cells became positive for nestin at day 6 and 15. The immunoreaction was present in filaments except in dividing cells. The presence of messenger RNA transcript for nestin was shown by reverse transcription polymerase chain reaction and sequencing of amplified complementary DNA. We then compared the presence of nestin with that of other intermediate filament proteins and smooth muscle ␣-actin. Immunoblotting showed that the relative concentrations of nestin, desmin, and vimentin increased between day 2 and 6 in primary culture. After the initial increase vimentin leveled off, while nestin and desmin showed a tendency to decrease. This pattern was quite different from that of glial fibrillary acidic protein, which kept declining, and smooth muscle ␣-actin, which increased continuously up to day 13 in culture. We then studied the presence of nestin in normal and CCl 4 -injured rat liver. In normal liver, minimal immunoreaction for nestin was observed within the liver parenchyma. During induction of fibrosis by carbon tetrachloride, nestin-positive stellate cells appeared at 6 weeks, which was late in comparison with the induction of desmin and smooth muscle ␣-actin. We conclude that nestin is induced in stellate cells during transition from the quiescent to the activated phenotype; culture activation is a stronger stimulus than in vivo activation by injection of CCl 4 . Taken Hepatic stellate cells exert specific liver functions: storage of large amounts of retinyl esters, synthesis and breakdown of hepatic extracellular matrix, secretion of a variety of cytokines, and control of the diameter of the sinusoids.

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Colocalization of Nestin and Vimentin/Desmin in Skeletal Muscle Cells Demonstrated by Three-Dimensional Fluorescence Digital Imaging Microscopy

Cited by 105 publications

References 0 publications

Mitotic Reorganization of the Intermediate Filament Protein Nestin Involves Phosphorylation by cdc2 Kinase

Mitotic Reorganization of the Intermediate Filament Protein Nestin Involves Phosphorylation by cdc2 Kinase

Podocytes Contribute to the Formation of Glomerular Crescents

Class VI intermediate filament protein nestin is induced during activation of rat hepatic stellate cells

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