Mitotic Reorganization of the Intermediate Filament Protein Nestin Involves Phosphorylation by cdc2 Kinase

Sahlgren, Cecilia; Mikhailov, Andrey; Hellman, Jukka; Chou, Ying Hao; Lendahl, Urban; Goldman, Robert D.; Eriksson, John E.

doi:10.1074/jbc.m009669200

Cited by 110 publications

(123 citation statements)

References 60 publications

Supporting

Mentioning

112

Contrasting

Unclassified

Order By: Relevance

“…Consistent with this idea is the observation that interphase vimentin IFs in BHK-21 cells are rapidly disassembled when cells are exposed to low doses of the phosphatase inhibitor calyculin A, which elevates the phosphorylation level of vimentin (Eriksson et al, 1992). Furthermore, phosphorylation of nestin by MPF during mitosis has also been correlated with the reorganization of vimentin IFs in ST15A cells (Sahlgren et al, 2001). Although it has not been explored in this study, the phosphorylation of nestin may also play a role in the extent of disassembly of vimentin IFs during mitosis.…”

Section: Discussionsupporting

confidence: 65%

“…During mitosis, the majority of these filamentous networks were disassembled into nonfilamentous structures that in C6 -2 cells appeared as punctate structures surrounded by more diffuse staining (Figure 4, B and C). This latter morphological feature is very similar to the one seen in ST15A cells, which express a similar level of nestin (Sahlgren et al, 2001). In mitotic BHK-21 cells, the disassembled vimentin displayed a distinct punctate pattern (Figure 6, G-I).…”

Section: If Breakdown During Mitosis Is a General Feature Of Cells Exsupporting

confidence: 58%

“…Furthermore, it has been demonstrated that nestin is expressed in BHK-21 (Steinert et al, 1999) and ST15A (Sahlgren et al, 2001) cells. This led us to examine whether MDBK cells also express nestin and whether there is a correlation between the expression of nestin and the disassembly of mitotic vimentin networks in other cultured cell types.…”

Section: If Breakdown During Mitosis Is a General Feature Of Cells Exmentioning

confidence: 99%

“…The phosphorylation of vimentin by MPF is a universal feature of mitotic cells expressing vimentin. Yet the breakdown of vimentin networks during mitosis has been reported only in MDBK (Franke et al, 1982), BHK-21 (Rosevear et al, 1990), and ST15A (Sahlgren et al, 2001) cells. Therefore, additional factors, unique to these three cell lines, are required for the disassembly of IFs.…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Nestin Promotes the Phosphorylation-dependent Disassembly of Vimentin Intermediate Filaments During Mitosis

Chou

Khuon

Herrmann

et al. 2003

MBoC

Self Cite

175

153

View full text Add to dashboard Cite

The expression of the intermediate filament (IF) protein nestin is closely associated with rapidly proliferating progenitor cells during neurogenesis and myogenesis, but little is known about its function. In this study, we examine the effects of nestin expression on the assembly state of vimentin IFs in nestin-free cells. Nestin is introduced by transient transfection and is positively correlated with the disassembly of vimentin IFs into nonfilamentous aggregates or particles in mitotic but not interphase cells. This nestin-mediated disassembly of IFs is dependent on the phosphorylation of vimentin by the maturation/M-phase–promoting factor at ser-55 in the amino-terminal head domain. In addition, the disassembly of vimentin IFs during mitosis appears to be a unique feature of nestin-expressing cell types. Furthermore, when the expression of nestin is downregulated by the nestin-specific small interfering RNA in nestin-expressing cells, vimentin IFs remain assembled throughout all stages of mitosis. Previous studies suggest that nonfilamentous vimentin particles are IF precursors and can be transported rapidly between different cytoplasmic compartments along microtubule tracks. On the basis of these observations, we speculate that nestin may play a role in the trafficking and distribution of IF proteins and potentially other cellular factors to daughter cells during progenitor cell division

show abstract

Section: Discussionsupporting

confidence: 65%

Section: If Breakdown During Mitosis Is a General Feature Of Cells Exsupporting

confidence: 58%

Section: If Breakdown During Mitosis Is a General Feature Of Cells Exmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Nestin Promotes the Phosphorylation-dependent Disassembly of Vimentin Intermediate Filaments During Mitosis

Chou

Khuon

Herrmann

et al. 2003

MBoC

Self Cite

175

153

View full text Add to dashboard Cite

show abstract

“…In a neuronal stem cell line, nestin was found to be reorganized during mitosis in. 44 Phosphorylation of nestin by different kinases leads to disassembly of nestin filaments during mitosis 44 or development of neuronal precursor cells. 45 This role of nestin during MC proliferation is further supported by the additional findings that nestin expression is clearly regulated during cell-cycle progression and that nestin knockdown by siRNA does affect cell-cycle progression in cultured MCs.…”

Section: Nestin In the Injured Glomerulus C Daniel Et Almentioning

confidence: 99%

Nestin expression in repopulating mesangial cells promotes their proliferation

Daniel

Albrecht

Lüdke

et al. 2008

Laboratory Investigation

View full text Add to dashboard Cite

We investigated whether the intermediate filament protein and neural stem cell marker nestin characterizes the glomerular progenitor/reserve cell population immigrating the glomerulus after mesangial cell (MC) injury in the rat (antiThy1 nephritis). Nestin expression was investigated by immunohistochemistry and real-time PCR during anti-Thy1 nephritis. Migration and proliferation assays were used to characterize the function of nestin in isolated MCs after nestin knockdown by siRNA. After MC injury during anti-Thy1 nephritis, glomerular nestin was transiently increased during the repopulation phase. At the peak of mesangial proliferation and expansion (day 5) most OX-7-positive MCs expressed nestin largely colocalizing with the activation marker a-smooth muscle actin and the proliferation marker PCNA. In contrast to a healthy, non-injured mesangium in vivo, MCs in culture are considered to be in an 'activated, injured state' and express nestin in a generalized distribution with condensed localization around the nucleus as well as intensive staining of cell protrusions such as filopodia. During cell cycle, the percentage of MCs with high nestin levels was increased during S-and G2-phase. Blocking of nestin using specific siRNA resulted in inhibition of cell proliferation but not cell migration. In conclusion, nestin is constitutively expressed in podocytes, but is a marker for repopulating MCs after experimental MC injury in vivo. Nestin promotes MC proliferation in vitro, suggesting a supporting role for nestin during repair reaction.

show abstract

Intermediate filaments in developing neurons: Beyond structure

Bott

Winckler

2020

Cytoskeleton

View full text Add to dashboard Cite

Neuronal development relies on a highly choreographed progression of dynamic cellular processes by which newborn neurons migrate, extend axons and dendrites, innervate their targets, and make functional synapses. Many of these dynamic processes require coordinated changes in morphology, powered by the cell's cytoskeleton. Intermediate filaments (IFs) are the third major cytoskeletal elements in vertebrate cells, but are rarely considered when it comes to understanding axon and dendrite growth, pathfinding and synapse formation. In this review, we first introduce the many new and exciting concepts of IF function, discovered mostly in non‐neuronal cells. These roles include dynamic rearrangements, crosstalk with microtubules and actin filaments, mechano‐sensing and ‐transduction, and regulation of signaling cascades. We then discuss the understudied roles of neuronally expressed IFs, with a particular focus on IFs expressed during development, such as nestin, vimentin and α‐internexin. Lastly, we illustrate how signaling modulation by the unconventional IF nestin shapes neuronal morphogenesis in unexpected and novel ways. Even though the first IF knockout mice were made over 20 years ago, the study of the cell biological functions of IFs in the brain still has much room for exciting new discoveries.

show abstract

Mitotic Reorganization of the Intermediate Filament Protein Nestin Involves Phosphorylation by cdc2 Kinase

Cited by 110 publications

References 60 publications

Nestin Promotes the Phosphorylation-dependent Disassembly of Vimentin Intermediate Filaments During Mitosis

Nestin Promotes the Phosphorylation-dependent Disassembly of Vimentin Intermediate Filaments During Mitosis

Nestin expression in repopulating mesangial cells promotes their proliferation

Intermediate filaments in developing neurons: Beyond structure

Contact Info

Product

Resources

About