Colloidal suspensions of clay or titanium dioxide nanoparticles can inhibit leaf growth and transpiration via physical effects on root water transport

Asli, Sare; Neumann, Peter M.

doi:10.1111/j.1365-3040.2009.01952.x

Cited by 470 publications

(216 citation statements)

References 39 publications

(78 reference statements)

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“…Another reason for the observed changes might be due to the inhibition of water and nutrient uptake as a result of CuO NPs exposure. A previous study by Asli and Neumann (2009) showed that titanium dioxide nanoparticles interfered with the ability of maize by forming aggregates along the root cell walls.…”

Section: Discussionmentioning

confidence: 98%

Evaluation of stress effects of copper oxide nanoparticles in Brassica napus L. seedlings

Nair

Chung

2017

3 Biotech

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Rapid growth of nanotechnology has enabled the production and use of engineered nanoparticles (ENPs) in several industries as well as in agriculture areas. This has raised ecotoxicological concerns due to the release of ENPs to the environment. In the present study, we investigated the effects of interactions of copper oxide nanoparticles (CuO NPs) on physiological, biochemical, and molecular indices in seedlings of an important oil seed crop Brassica napus L. The seedlings were treated with 0, 20, 50, 100, 200, 400, and 500 mg/L of CuO NPs for 14 days in halfstrength semi-solid Murashige and Skoog medium. The CuO NPs treatment significantly reduced shoot and root growth as well as plant biomass. Shortening and thickening of primary and lateral roots and inhibition of lateral root growth was observed at higher concentrations. An increase in reactive oxygen species generation, and malondialdehyde accumulation was observed. Histochemical staining of roots with propidium iodide and aniline blue indicated cell death and callose formation in roots. Transcriptional modulation of genes related to oxidative stress viz. CuZn superoxide dismutase, catalase, and ascorbate peroxidase was observed. Element content analysis showed an increase in Cu content and decrease in Fe, Mn, and Zn contents. Overall, exposure to CuO NPs caused oxidative injury, cell death, callose formation, and decreased the micro nutrient contents in B. napus seedlings.

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Section: Discussionmentioning

confidence: 98%

Evaluation of stress effects of copper oxide nanoparticles in Brassica napus L. seedlings

Nair

Chung

2017

3 Biotech

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“…Toxicity tests using hydroponics to expose terrestrial plants do show some toxic effects [97]. In comparison, artificial or natural soils (OECD 208, terrestrial plant test) usually result in little or no phytotoxcity [98,99]. Commonly used endpoints such as germination and root growth have so far shown limited sensitivity to ENMs, even in hydroponic settings [100].…”

Section: Sensitive Endpoints For Soil Organismmentioning

confidence: 99%

Ecotoxicity test methods for engineered nanomaterials: Practical experiences and recommendations from the bench

Handy

Cornelis

Fernandes

et al. 2011

Enviro Toxic and Chemistry

297

236

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Abstract-Ecotoxicology research is using many methods for engineered nanomaterials (ENMs), but the collective experience from researchers has not been documented. This paper reports the practical issues for working with ENMs and suggests nano-specific modifications to protocols. The review considers generic practical issues, as well as specific issues for aquatic tests, marine grazers, soil organisms, and bioaccumulation studies. Current procedures for cleaning glassware are adequate, but electrodes are problematic. The maintenance of exposure concentration is challenging, but can be achieved with some ENMs. The need to characterize the media during experiments is identified, but rapid analytical methods are not available to do this. The use of sonication and natural/synthetic dispersants are discussed. Nano-specific biological endpoints may be developed for a tiered monitoring scheme to diagnose ENM exposure or effect. A case study of the algal growth test highlights many small deviations in current regulatory test protocols that are allowed (shaking, lighting, mixing methods), but these should be standardized for ENMs. Invertebrate (Daphnia) tests should account for mechanical toxicity of ENMs. Fish tests should consider semistatic exposure to minimize wastewater and animal husbandry. The inclusion of a benthic test is recommended for the base set of ecotoxicity tests with ENMs. The sensitivity of soil tests needs to be increased for ENMs and shortened for logistics reasons; improvements include using Caenorhabditis elegans, aquatic media, and metabolism endpoints in the plant growth tests. The existing bioaccumulation tests are conceptually flawed and require considerable modification, or a new test, to work for ENMs. Overall, most methodologies need some amendments, and recommendations are made to assist researchers. Environ. Toxicol. Chem. 2012;31:15-31. # 2011 SETAC

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“…Uptake of nanoparticles into plant leaves has been proposed to occur through small pores in the cuticle (< 5 nm) or through stomatal apertures (21). Cell wall pores are smaller than ten nanometers and the cell wall is expected to be a tight sieve which does not allow nanoparticles migration (34). Furthermore, it should be considered that translocation of nanoparticles from one leaf to another would require complex translocation mechanisms.…”

Section: Translocation Of Nanoparticlesmentioning

confidence: 99%

No Evidence for Cerium Dioxide Nanoparticle Translocation in Maize Plants

Birbaum

Brogioli

Schellenberg

et al. 2010

Environ. Sci. Technol.

249

149

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The rapidly increasing production of engineered nanoparticles has raised questions regarding their environmental impact and their mobility to overcome biological important barriers. Nanoparticles were found to cross different mammalian barriers, which is summarized under the term translocation. The present work investigates the uptake and translocation of cerium dioxide nanoparticles into maize plants as one of the major agricultural crops. Nanoparticles were exposed either as aerosol or as suspension. Our study demonstrates that 50 microgram cerium per gram leaves was either adsorbed or incorporated into maize leaves. This amount could not be removed by a washing step and did not depend on closed or open stomata investigated under dark and light exposure conditions. However, no translocation into newly grown leaves was found when cultivating the maize plants after airborne particle exposure. The use of inductively coupled mass spectrometer allowed detection limits of less than 1 nanogram cerium per gram leaf. Exposure of plants to well characterized nanoparticle suspensions in the irrigation water resulted also in no detectable translocation. These findings may indicate that the biological barriers of plants are more resistant against nanoparticle translocation than mammalian barriers.

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Colloidal suspensions of clay or titanium dioxide nanoparticles can inhibit leaf growth and transpiration via physical effects on root water transport

Cited by 470 publications

References 39 publications

Evaluation of stress effects of copper oxide nanoparticles in Brassica napus L. seedlings

Evaluation of stress effects of copper oxide nanoparticles in Brassica napus L. seedlings

Ecotoxicity test methods for engineered nanomaterials: Practical experiences and recommendations from the bench

No Evidence for Cerium Dioxide Nanoparticle Translocation in Maize Plants

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