Colloidal gold conjugated monoclonal antibodies, studied in the BIAcore biosensor and in the Nycocard immunoassay format

Dual-channel differential surface plasmon ellipsometry for bio-chemical sensing

“…4(b) it is linear over a range of 3.5 × 10 −3 RIU which is more than adequate for the monitoring of biological interactions at the surface. (Johne et al, 1995;Stöcklein et al, 2000;Papalia et al, 2006;Englebienne et al, 2003).…”

Application of gold-labeled antibody biosensor in simultaneous determination of total aflatoxins using artificial neural network

“…The immunoreactivity of antibody depends upon the tertiary structure of the antibody remaining unperturbed after formation of conjugates with GNP [43]. The tertiary structure of the antibody can be studied by fluorescence measurements, by exciting the sample at a particular wavelength and monitoring the fluorescence emission from the tryptophan or tyrosine residues in the antibody.…”

A Lateral Flow Smart Phone Image Analysis Diagnostic

“…Johne et al (1995) demonstrated that the BIAcore™ SPR is compatible with gold conjugated proteins. It is able to view the molecular interactions without interference from the gold particles [7].…”

“…Various conjugation techniques will affect the binding of antigen with antibody [7]. The goal is to find a high affinity antibody to successfully and sensitively capture antigens from an analyte [7].…”

“…The goal is to find a high affinity antibody to successfully and sensitively capture antigens from an analyte [7]. Sol (gold) Particle Immunoassay (SPIA) has been utilized with numerous antibodies including, immunoglobin G, immunoglobins from blood of HIV positive patients and cystatin C. BIAcore™ Surface Plasmon Resonance (SPR) is a technology that has the ability to study interacting molecules that are associating and dissociating.…”