Collective cell migration driven by filopodia—New insights from the social behavior of myotubes

Bischoff, Maik C.; Bogdan, Sven

doi:10.1002/bies.202100124

Cited by 9 publications

(10 citation statements)

References 126 publications

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“…It is well-known that the newly formed filopodia as a key factor is associated with cell migration and metastasis [ 32 ]. Generally, field-induced responses involving actin cytoskeleton reassembly will inevitably result in morphological reorganization, which can be directly investigated by the percentage of actin polymerization (ratio of cells with actin reassembly to total number of cells) and the surface area of cells.…”

Section: Resultsmentioning

confidence: 99%

A Novel and Versatile Microfluidic Device for Cell Assays under Radio Frequency Exposure

et al. 2023

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Wound healing is a complex process composed of different stages, which involves extensive communication between the different cellular factors of the extracellular matrix (ECM). The radio frequency electromagnetic field (RF-EMF) has been used to accelerate the wound-healing process and it has been found to enhance cell alignment and mobility. The conventional methods for cell mobility analysis in an electromagnetic field generated by a radiation source are not advisable due to the low-precision, nonuniform distribution of the field, low efficiency of the analysis in batch and the lack of system integration for autonomous on-body operation. Here, a novel and versatile electromagnetic exposure system integrated with a microfluidic chip was fabricated to explore the EMF-induced response. A gradient electromagnetic field in a two-dimensional plane has been successfully established in the microchambers placed along the field line. In this work, by deploying our radiation experiments in vitro, we validated the on-chip monitoring of cell response to exposure. This electromagnetic field was simulated and human amniotic epithelial cells (HAECs) were cultured in different microchambers for continuous exposure to the electromagnetic field excited by a monopole RF antenna (1.8 GHz). New protrusions were generated and an obvious increase in filopodia with the increased field intensity was investigated. Meanwhile, the variation in intracellular Ca2+ concentration under the electromagnetic field was examined. The inhibitory effect of the Ca2+ circulation was further inspected to reveal the potential downstream signaling pathway in the RF-EMF-related bioassay, suggesting that cytoskeletal dynamics of cells under exposure are highly associated with the EGF receptor (EGFR)-cytoskeleton downstream signaling pathway. Finally, the field-induced cell elongation and alignment parallel to the field direction were observed. Additionally, the subsequent recovery (field withdrawal) and re-establishment (field re-exposure) were explored. These results indicated that this reliable and versatile exposure system for bioassay could achieve precise and high-throughput detection of the RF-EMF-induced cytoskeletal reorganization in vitro and evaluate the possible health risk from RF-EMF exposure.

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Section: Resultsmentioning

confidence: 99%

A Novel and Versatile Microfluidic Device for Cell Assays under Radio Frequency Exposure

et al. 2023

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“…[46] In short, contact inhibition of migration is the mechanism by which a cell stops or changes the direction of migration when it collides with another cell, [47][48][49][50] while contact stimulation of migration is the mechanism by which one cell contacts another cell to activate cell migration. [51][52][53] From Figure 6b, it was found that for the undifferentiated cells on the 22 kPa hydrogel, one cell encountering another cell would change the direction of migration (contact inhibition of migration). However, for undifferentiated cells at 22 kPa with AS, one cell contacting another cell could activate the cell migration (contact stimulation of migration).…”

Section: Synergistic Effect Of Matrix and Fss On Cell Migrationmentioning

confidence: 99%

Effect of Hydrogel Matrix and Fluid Shear Stress on the Behavioral Regulation of Mesenchymal Stem Cells

Kang,

Wu,

et al. 2024

Small Structures

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Development of a micromodel that recapitulates multiple mechanical properties to mimic the complex mechanical microenvironment is crucial for cell‐based research. Herein, a microsystem combining structure of hydrogel matrix and acoustic streaming (AS) to mimic the cellular microenvironment is proposed, which can realize multiplex cellular mechanical cues, including matrix stiffness, fluid shear stress (FSS) generated by AS, and matrix roughness. The results demonstrate that the cell spreading area enlarges with the increase of matrix stiffness, and cell spreading is encouraged by integrin β1 cluster to polymerize actin when combines the hydrogel matrix with FSS. In addition, FSS has the influence on the roughness of the hydrogel, which further affects the cell morphology and mechanical properties, inducing mesenchymal stem cells (MSCs) differentiation into astrocytes rapidly. Meanwhile, cell migration is also enhanced by FSS stimulation, particularly, undifferentiated cells at 22 kPa hydrogel have the fastest migration speed, and change the movement model from contact inhibition to contact stimulation migration. Especially, matrix roughness and stiffness dominantly control of cell spreading and differentiation, whereas FSS affects cell migration. In conclusion, the microsystem in this work shows superior performance in regulating the spreading, differentiation, and migration of MSCs, which provides a new tool for cell‐microenvironment study.

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“…Based on widely reported cell migration studies, it was confirmed that the fundamental mechanics of single-cell migration may be applied to collective movement, as well. During group migration, the cells stay attached throughout the process, allowing cell–cell junctions to remain intact during movement. ,,,− Collective migratory behaviors occur in response to a variety of environmental limitations, which result in a range of morphological characteristics. They include the presence of mesenchymal leader cells that are highly motile, cryptic lamellipodia development by cells placed a few rows behind the leading front and the formation of small groups of follower cells that are directed by the leader ones .…”

Section: Cell Migrationmentioning

confidence: 99%

“…They are in charge of controlling the direction of movement, moreover they support persistence of migratory cells via increasing cell−matrix adhesion at the leading edge. 38 2.2. Collective Cell Migration.…”

Section: Cell Migrationmentioning

confidence: 99%

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Unraveling Endothelial Cell Migration: Insights into Fundamental Forces, Inflammation, Biomaterial Applications, and Tissue Regeneration Strategies

Jerka,

Bonowicz,

Piekarska

et al. 2024

ACS Appl. Bio Mater.

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Cell migration is vital for many fundamental biological processes and human pathologies throughout our life. Dynamic molecular changes in the tissue microenvironment determine modifications of cell movement, which can be reflected either individually or collectively. Endothelial cell (EC) migratory adaptation occurs during several events and phenomena, such as endothelial injury, vasculogenesis, and angiogenesis, under both normal and highly inflammatory conditions. Several advantageous processes can be supported by biomaterials. Endothelial cells are used in combination with various types of biomaterials to design scaffolds promoting the formation of mature blood vessels within tissue engineered structures. Appropriate selection, in terms of scaffolding properties, can promote desirable cell behavior to varying degrees. An increasing amount of research could lead to the creation of the perfect biomaterial for regenerative medicine applications. In this review, we summarize the state of knowledge regarding the possible systems by which inflammation may influence endothelial cell migration. We also describe the fundamental forces governing cell motility with a specific focus on ECs. Additionally, we discuss the biomaterials used for EC culture, which serve to enhance the proliferative, proangiogenic, and promigratory potential of cells. Moreover, we introduce the mechanisms of cell movement and highlight the significance of understanding these mechanisms in the context of designing scaffolds that promote tissue regeneration.

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Collective cell migration driven by filopodia—New insights from the social behavior of myotubes

Cited by 9 publications

References 126 publications

A Novel and Versatile Microfluidic Device for Cell Assays under Radio Frequency Exposure

A Novel and Versatile Microfluidic Device for Cell Assays under Radio Frequency Exposure

Effect of Hydrogel Matrix and Fluid Shear Stress on the Behavioral Regulation of Mesenchymal Stem Cells

Unraveling Endothelial Cell Migration: Insights into Fundamental Forces, Inflammation, Biomaterial Applications, and Tissue Regeneration Strategies

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