Collection of villous tissue under ultrasound guidance to improve the cytogenetic study of early pregnancy failure

Greenwold, Natalie; Jauniaux, Eric

doi:10.1093/humrep/17.2.452

Cited by 45 publications

(35 citation statements)

References 22 publications

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“…Therefore cells from 60% (18/30) of all failed pregnancies received in the laboratory were successfully cultured in at least one media type. These figures compare favourably to the 72.3% culture success rate of material from first trimester losses in women [3].…”

Section: Resultssupporting

confidence: 64%

“…AmnioChrome™ Plus (Amnio) and Chang D ® (Chang) are commercial media developed for the primary culture of amniotic fluid and chorionic villi cells for the purpose of karyotyping. Both have previously been shown to be successful for this purpose [3]. Equine trophoblast medium (EqTM) is a medium used in our laboratory for the routine culture of equine trophoblast cells derived from normal early pregnancies [10].…”

Section: Methodsmentioning

confidence: 99%

“…Early pregnancy loss (EPL) remains a significant cause of reproductive wastage in mammals [1][2][3][4][5] but the underlying aetiologies are often unknown [6,7]. A retrospective study in horses showed a speculative cause for EPL was found in just 16% of cases [8].…”

Section: Introductionmentioning

confidence: 99%

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A method for isolating and culturing placental cells from failed early equine pregnancies

et al. 2016

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Section: Resultssupporting

confidence: 64%

Section: Methodsmentioning

confidence: 99%

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A method for isolating and culturing placental cells from failed early equine pregnancies

et al. 2016

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“…However, this material is often unsuitable for analysis with reliable results, either due to its poor quality or the small number of cells obtained in culture (Danielly et al, 1998;Lomax et al, 2000;Greenwold and Jauniaux, 2002).…”

Section: Discussionmentioning

confidence: 99%

Array-CGH testing in spontaneous abortions with normal karyotypes

Borovik

Pérez²,

Silva

et al. 2008

Genet. Mol. Biol.

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In about 50% of first trimester spontaneous abortion the cause remains undetermined after standard cytogenetic investigation. We evaluated the usefulness of array-CGH in diagnosing chromosome abnormalities in products of conception from first trimester spontaneous abortions. Cell culture was carried out in short-and long-term cultures of 54 specimens and cytogenetic analysis was successful in 49 of them. Cytogenetic abnormalities (numerical and structural) were detected in 22 (44.89%) specimens. Subsequent, array-CGH based on large insert clones spaced at 1 Mb intervals over the whole genome was used in 17 cases with normal G-banding karyotype. This revealed chromosome aneuplodies in three additional cases, giving a final total of 51% cases in which an abnormal karyotype was detected. In keeping with other recently published works, this study shows that array-CGH detects abnormalities in a further~10% of spontaneous abortion specimens considered to be normal using standard cytogenetic methods. As such, array-CGH technique may present a suitable complementary test to cytogenetic analysis in cases with a normal karyotype.

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“…Post-evacuation tissue analysis and CVS evaluated the same tissue (placenta), although obtained by different techniques. Greenwold and Jauniaux [16] reported a comparison of 170 prospectively obtained villous samples from spontaneous pregnancy losses, obtained using ultrasound-guided biopsy forceps prior to uterine evacuation with 1,191 villous samples obtained from the actual uterine evacuation specimen (conventional products of conception). They found a significantly higher karyotype success rate (94.5%) in the villus samples collected at the beginning of the procedure versus traditional products of conception (83.7%).…”

Section: Commentmentioning

confidence: 99%

Value of Amniocentesis versus Fetal Tissue for Cytogenetic Analysis in Cases of Fetal Demise

et al. 2009

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Objective: Use of fetal tissue for cytogenetic analysis in cases of second- and third-trimester fetal demise frequently results in unacceptably high failure rates. We reviewed our ongoing use of amniocentesis prior to uterine evacuation to determine if this provided a better source of cells for cytogenetic analysis. Methods: We compared cytogenetic results using fetal tissues obtained following uterine evacuation to our ongoing use of amniotic fluid cell obtained by transabdominal amniocentesis prior to uterine evacuation from 2003 to 2008. Results: In 49 of the 63 cases evaluated by fetal tissue biopsies performed after uterine evacuation, a karyotypic analysis was obtained (77.8%). Among the 38 cases evaluated by amniocentesis, an amniotic fluid sample and fetal cytogenetic results were obtained in all 38 (100%) cases. Conclusion: Our findings indicate that amniocentesis is a more reliable source of cytogenetic information than fetal tissue in cases of second- and third-trimester fetal demise.

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Collection of villous tissue under ultrasound guidance to improve the cytogenetic study of early pregnancy failure

Abstract: Our data show that a karyotype can be obtained from clean villous material collected at the time of surgical evacuation of miscarriage. Thus, it is not justified to subject women to transabdominal chorionic villus sampling to achieve a high karyotype success rate.

Cited by 45 publications

References 22 publications

A method for isolating and culturing placental cells from failed early equine pregnancies

A method for isolating and culturing placental cells from failed early equine pregnancies

Array-CGH testing in spontaneous abortions with normal karyotypes

Value of Amniocentesis versus Fetal Tissue for Cytogenetic Analysis in Cases of Fetal Demise

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