Collection, handling, and shipment of microbiological specimens

Forney, John E.

doi:10.5962/bhl.title.112277

1969

DOI: 10.5962/bhl.title.112277

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Collection, handling, and shipment of microbiological specimens

John E. Forney¹

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1977

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Isolation of Francisella tularensis from infected frozen human blood

Pittman¹,

Shaw²,

Cherry³

1977

J Clin Microbiol

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Francisella tularensis was isolated from human blood that was frozen for 3 months before it was examined. Before he became ill, the patient operated a "bush-hog" in an area thickly populated with rabbits. His illness was undiagnosed and untreated before his death. Portions of blood and tissue homogenates from necropsy were injected intraperitoneally into mice and inoculated onto glucose-cysteine-blood agar plates. F. tularensis did not grow from the culture plates, but mice inoculated with the blood died in 48 to 72 h. Fluorescent-antibody stains of mouse liver and spleen impression smears showed clumps of cells or amorphous masses of brightly staining envelope material around the cells. Tissue impressions of liver, spleen, and heart blood inoculated onto glucose-cysteine-blood agar yielded pure cultures of F. tularensis.

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Isolation of Francisella tularensis from infected frozen human blood

Pittman¹,

Shaw²,

Cherry³

1977

J Clin Microbiol

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show abstract

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Collection, handling, and shipment of microbiological specimens

Cited by 1 publication

References 0 publications

Isolation of Francisella tularensis from infected frozen human blood

Isolation of Francisella tularensis from infected frozen human blood

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