Collection, Cryopreservation, and Characterization of Human Dental Pulp–Derived Mesenchymal Stem Cells for Banking and Clinical Use

Abstract

Recent studies have shown that mesenchymal stem cells (MSC) with the potential for cell-mediated therapies and tissue engineering applications can be isolated from extracted dental tissues. Here, we investigated the collection, processing, and cryobiological characteristics of MSC from human teeth processed under current good tissue practices (cGTP). Viable dental pulp-derived MSC (DPSC) cultures were isolated from 31 of 40 teeth examined. Of eight DPSC cultures examined more thoroughly, all expressed appropriate cell surface markers and underwent osteogenic, adipogenic, and chondrogenic differentiation in appropriate differentiation medium, thus meeting criteria to be called MSC. Viable DPSC were obtained up to 120 h postextraction. Efficient recovery of DPSC from cryopreserved intact teeth and second-passage DPSC cultures was achieved. These studies indicate that DPSC isolation is feasible for at least 5 days after tooth extraction, and imply that processing immediately after extraction may not be required for successful banking of DPSC. Further, the recovery of viable DPSC after cryopreservation of intact teeth suggests that minimal processing may be needed for the banking of samples with no immediate plans for expansion and use. These initial studies will facilitate the development of future cGTP protocols for the clinical banking of MSC.

Induced in vitro differentiation of neural-like cells from human exfoliated deciduous teeth-derived stem cells

“…This presents a far better alternative to simply discarding the teeth or storing them as mementos from the past. Therefore, there are many interests in banking person-specific tooth-derived stem cells (Arora et al, 2009;Perry et al, 2008). The proliferation rate of SHED is significantly higher than that of DPSCs and BM-MSCs (Nakamura et al, 2009;Takeda et al, 2008).…”

“…It is interesting to note that DPSC isolation is possible for at least five days after tooth extraction, which implies that immediate processing after extraction may not be necessary for successful DPSC banking. Further, the recovery of viable DPSCs after cryopreserving intact teeth suggests that minimal processing may be needed for the banking of samples that have no immediate plans for expansion and use (Perry et al, 2008). Even after cryopreservation these cells were capable to differentiate into neural, osteocytes/odontocytes, adipocytes, myocytes, and chondrocytes (Zhang et al, 2006) under related condition.…”

Mesenchymal Stem Cells and Platelet Derived Concentrates in Regenerative Medicine

“…Mesenchymal stem cells are a significant type of adult stem cells that they can extracted from a variety of tissue ( Figure 1), including adipose tissue [1][2][3][4], skeletal muscle tissue [5], synovial membranes [6], cervical tissue [7], saphenous veins [8], periodontal ligaments [9], Wharton's jelly [10], umbilical cord blood [11], umbilical cords [12], amniotic fluid [13], dental pulp [14], placentae [15], lung tissue [16,17], liver tissue [18,19], and dermal tissue [19].…”

A Importância Da Odontologia Nas Pesquisas Em Células-Tronco

“…(13) As Células Mononucleares de Medula Óssea (CMO) compreendem as células hematopoiéticas e as não-hematopoiéticas, ambas possuindo plasticidade, potencial de auto-renovação e diferenciação e por isso tem sido sugerido a utilização de CMO em terapia celular. (15) Foi demonstrado que o transplante de células da medula óssea pode resultar na completa e permanente reconstituição dos tecidos hematopoiéticos e que essas células podem se diferenciar em célu-las epiteliais do fígado, baço, trato gastrintestinal, pele e pulmões. (10) Outros estudos demonstraram que células da medula óssea possuem capacidade de se diferenciar em microglia e astrócitos após transplante em camundongo (15) e alguns trabalhos reportaram cé-lulas de medula óssea expressando marcadores neuronais.…”

“…(15) Foi demonstrado que o transplante de células da medula óssea pode resultar na completa e permanente reconstituição dos tecidos hematopoiéticos e que essas células podem se diferenciar em célu-las epiteliais do fígado, baço, trato gastrintestinal, pele e pulmões. (10) Outros estudos demonstraram que células da medula óssea possuem capacidade de se diferenciar em microglia e astrócitos após transplante em camundongo (15) e alguns trabalhos reportaram cé-lulas de medula óssea expressando marcadores neuronais. (10) CÉLULAS-TRONCO DA POLPA DE DENTE HUMANO O dente se desenvolve na maxila e mandíbula dos mamíferos, variando a morfologia de acordo com a espécie e a localização, mas a estrutura se mantém similar sendo formado por: esmalte, cemento e dentina (juntos formam o tecido mineralizado que reveste a polpa), a polpa (formada por tecido conjuntivo vascularizado, com diferentes tipos celulares) e o periodonto (fixa os dentes nos ossos maxilares e mandibulares.…”